Abstract
A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.
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- AINIS H., JAMESON E., RYAN R. M. Action of guinea pig serum and human gamma globulin on the growth of a rat tumor. Science. 1956 Nov 16;124(3229):980–981. doi: 10.1126/science.124.3229.980. [DOI] [PubMed] [Google Scholar]
- AINIS H., KURTZ H. M., KRAMER P. I., WEIMER H. E., RYAN R. M., JAMESON E. In vivo and in vitro studies of the action of guinea pig serum against the ascites form of the MurphySturm lymphosarcoma. Cancer Res. 1958 Dec;18(11):1309–1313. [PubMed] [Google Scholar]
- KAPLAN S. L., GRUMBACH M. M. Electrophoretic and immunological characteristics of native and purified human growth hormone. Nature. 1962 Oct 27;196:336–338. doi: 10.1038/196336a0. [DOI] [PubMed] [Google Scholar]
- KEKWICK R. A. The serum proteins of the fetus and young of some mammals. Adv Protein Chem. 1959;14:231–254. doi: 10.1016/s0065-3233(08)60612-9. [DOI] [PubMed] [Google Scholar]
- KIDD J. G. Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. I. Course of transplanted cancers of various kinds in mice and rats given guinea pig serum, horse serum, or rabbit serum. J Exp Med. 1953 Dec;98(6):565–582. doi: 10.1084/jem.98.6.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIDD J. G. Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. II. Studies on the nature of the active serum constituent: histological mechanism of the regression: tests for effects of guinea pig serum on lymphoma cells in vitro: discussion. J Exp Med. 1953 Dec;98(6):583–606. doi: 10.1084/jem.98.6.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUNKEL H. G., SLATER R. J. Zone electrophoresis in a starch supporting medium. Proc Soc Exp Biol Med. 1952 May;80(1):42–44. doi: 10.3181/00379727-80-19516. [DOI] [PubMed] [Google Scholar]
- KUNKEL H. G., TISELIUS A. Electrophoresis of proteins on filter paper. J Gen Physiol. 1951 Sep;35(1):89–118. doi: 10.1085/jgp.35.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEDOUX L. Action of ribonuclease on certain ascites tumours. Nature. 1955 Feb 5;175(4449):258–259. doi: 10.1038/175258b0. [DOI] [PubMed] [Google Scholar]
- LINDSKOG S. Purification and properties of bovine erythrocyte carbonic anhydrase. Biochim Biophys Acta. 1960 Apr 8;39:218–226. doi: 10.1016/0006-3002(60)90156-6. [DOI] [PubMed] [Google Scholar]
- MEISTER A., LEVINTOW L., GREENFIELD R. E., ABENDSCHEIN P. A. Hydrolysis and transfer reactions catalyzed by omega-amidase preparations. J Biol Chem. 1955 Jul;215(1):441–460. [PubMed] [Google Scholar]