Abstract
The in vitro anamnestic antibody response of popliteal lymph node fragments to additions of antigen closely resembles the in vivo anamnestic antibody response in its sensitivity to antigen, in the time course of antibody production, and in the sequence of appearance and the morphology of the antibody containing cells. Most of the cells responsible for antibody synthesis remain in the explant and do not migrate, although a few can be found in the outgrowing sheet of cells. The smallest concentration of bovine serum albumin which stimulates an anamnestic response in vitro is about 1 x 10–9 gm/ml.
Full Text
The Full Text of this article is available as a PDF (582.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COONS A. H., LEDUC E. H., CONNOLLY J. M. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955 Jul 1;102(1):49–60. doi: 10.1084/jem.102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DIXON F. J., MAURER P. H., WEIGLE W. O., DEICHMILLER M. P. Rates of antibody synthesis during first, second, and hyperimmune responses of rabbits to bovine gamma globulin. J Exp Med. 1956 Apr 1;103(4):425–438. doi: 10.1084/jem.103.4.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GARVEY J. S., CAMPBELL D. H. The relation of circulating antibody concentration to localization of labeled (S35) antigen. J Immunol. 1954 Feb;72(2):131–138. [PubMed] [Google Scholar]
- LEDUC E. H., COONS A. H., CONNOLLY J. M. Studies on antibody production. II. The primary and secondary responses in the popliteal lymph node of the rabbit. J Exp Med. 1955 Jul 1;102(1):61–72. doi: 10.1084/jem.102.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCDEVITT H. O., PETERS J. H., POLLARD L. W., HARTER J. G., COONS A. H. PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY. J Immunol. 1963 Apr;90:634–642. [PubMed] [Google Scholar]
- MICHAELIDES M. C., COONS A. H. Studies on antibody production. V. The secondary response in vitro. J Exp Med. 1963 Jun 1;117:1035–1051. doi: 10.1084/jem.117.6.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]