Abstract
The incubation of endotoxin with Na2Cr51O4 yielded a product which was well labeled. That the label was fixed on the endotoxin itself was shown by autoradiography on specific lines of precipitation formed in agar. Ultracentrifugation at 40,000 RPM sedimented 80 per cent of the total weight of the starting Boivin preparation. Agar diffusion patterns with subsequent autoradiographs demonstrated that the chromium tag was associated only with the heavy fractions of the pellet. The supernatant contained precipitable, but unlabeled endotoxin. Toxicity measurements showed that more than 99 per cent of the total toxicity resided in the pellet fractions. The chromate-tagged endotoxin was specifically identified in plasma samples taken up to 6 hours after intravenous administration of LD 50 or sublethal doses. The endotoxin was not totally detoxified in vivo since plasma collected 6 hours after the injection of even the sublethal dose was toxic when assayed in adrenalectomized mice. The endotoxin was specifically identified in urine specimens but it was no longer toxic or radioactive. Agar diffusion experiments indicated that only degraded material was present.
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Selected References
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- BRAUDE A. I., CAREY F. J., SUTHERLAND D., ZALESKY M. Studies with radioactive endotoxin. I. The use of Cr51 to label endotoxin of Escherichia coli. J Clin Invest. 1955 Jun;34(6):850–857. doi: 10.1172/JCI103140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAUDE A. I., CAREY F. J., ZALESKY M. Studies with radioactive endotoxin. II. Correlation of physiologic effects with distribution of radioactivity in rabbits injected with radioactive sodium chromate. J Clin Invest. 1955 Jun;34(6):858–866. doi: 10.1172/JCI103141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHEDID L., BOYER F., PARANT M. [Study of the abortive action of endotoxins injected into the normal, castrated or hypophysectomized pregnant female mouse]. Ann Inst Pasteur (Paris) 1962 Jan;102:77–84. [PubMed] [Google Scholar]
- HOWARD J. G., BIOZZI G., HALPERN B. N., STIFFEL C., MOUTON D. The effect of Mycobacterium tuberculosis (BCG) infection on the resistance of mice to bacterial endotoxin and Salmonella enteritidis infection. Br J Exp Pathol. 1959 Jun;40(3):281–290. [PMC free article] [PubMed] [Google Scholar]
- NOYES H. E., McINTURF C. R., BLAHUTA G. J. Studies on distribution of Escherichia coli endotoxin in mice. Proc Soc Exp Biol Med. 1959 Jan;100(1):65–68. doi: 10.3181/00379727-100-24525. [DOI] [PubMed] [Google Scholar]
- PRINS H. K. The binding of Cr-51 by human erythrocytes. Vox Sang. 1962;7:370–372. doi: 10.1111/j.1423-0410.1962.tb03265.x. [DOI] [PubMed] [Google Scholar]
- ROWLEY D., ALI W., JENKIN C. R. A reaction between fresh serum and lipopolysaccharides of gram-negative bacteria. Br J Exp Pathol. 1958 Feb;39(1):90–98. [PMC free article] [PubMed] [Google Scholar]
- ROWLEY D., HOWARD J. G., JENKIN C. R. The fate of 32P-labelled bacterial lipopolysaccharide in laboratory animals. Lancet. 1956 Apr 7;270(6919):366–367. doi: 10.1016/s0140-6736(56)90110-6. [DOI] [PubMed] [Google Scholar]
- SMITH R. T., BRAUDE A. I., CAREY F. J. The distribution of Cr51 labeled E. coli endotoxin in the generalized Shwartzman reaction. J Clin Invest. 1957 May;36(5):695–699. doi: 10.1172/JCI103471. [DOI] [PMC free article] [PubMed] [Google Scholar]