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. 1963 Mar 31;117(4):603–620. doi: 10.1084/jem.117.4.603

THE ASSOCIATION OF SKIN-SENSITIZING ANTIBODY WITH THE β2A-GLOBULINS IN SERA FROM RAGWEED-SENSITIVE PATIENTS

Philip Fireman 1, Wilton E Vannier 1, Howard C Goodman 1
PMCID: PMC2137617  PMID: 13945231

Abstract

1. The removal of the β2A-globulins from three sera from treated ragweed-sensitive individuals by immune absorption was associated with the loss of all detectable skin-sensitizing antibody activity as demonstrated by Prausnitz-Küstner testing. 2. Gel filtration studies, with sephadex G-200, indicated that the fractions containing only macroglobulins were devoid of all detectable skin-sensitizing antibody activity. 3. The immune absorption of the γ-globulins from a serum fraction containing β2A-globulins, γ-globulins, and a trace of β2M-globulins had no detectable influence on the skin-sensitizing antibody activity. 4. The removal of a portion of the albumin from the allergic sera by immune absorption, with retention of the skin-sensitizing activity, indicated that the loss of skin-sensitizing antibody was not due to non-specific absorption on an antigen-antibody precipitate. 5. No inhibition of the Prausnitz-Küstner reaction was observed when sheep serum, normal human serum, or normal human γ-globulins were tested in concentrations described. 6. We conclude that the skin-sensitizing antibody activities in the three sera from treated ragweed-sensitive individuals studied were associated with the β2A-globulins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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