Abstract
The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free homogenate was retained in its RNA fraction isolated by means of the phenol method. No antibody formation occurred if such RNA was treated with RNAase. On sucrose gradients (5 to 20 per cent), the active RNA was found to be present in the top third layer. The question of the possible presence of antigen complexed to the RNA is discussed.
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- AKSENOVA N. N., BRESLER V. M., VOROBYEV V. I., OLENOV J. M. Influence of ribonucleic acids from the liver on implantation and growth of transplantable tumours. Nature. 1962 Nov 3;196:443–444. doi: 10.1038/196443a0. [DOI] [PubMed] [Google Scholar]
- ALGIRE G. H., BORDERS M. L., EVANS V. J. Studies of heterografts in diffusion chambers in mice. J Natl Cancer Inst. 1958 Jun;20(6):1187–1201. doi: 10.1093/jnci/20.6.1187. [DOI] [PubMed] [Google Scholar]
- AMOS D. B., WAKEFIELD J. D. Growth of mouse ascites tumor cells in diffusion chambers. I. Studies of growth rate of cells and of the rate of entry of antibody. J Natl Cancer Inst. 1958 Oct;21(4):657–670. [PubMed] [Google Scholar]
- FISHMAN M. Antibody formation in tissue culture. Nature. 1959 Apr 25;183(4669):1200–1201. doi: 10.1038/1831200a0. [DOI] [PubMed] [Google Scholar]
- FISHMAN M. Antibody formation in vitro. J Exp Med. 1961 Dec 1;114:837–856. doi: 10.1084/jem.114.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GARVEY J. S., CAMPBELL D. H. The retention of S35-labelled bovine serum albumin in normal and immunized rabbit liver tissue. J Exp Med. 1957 Apr 1;105(4):361–372. doi: 10.1084/jem.105.4.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
- MANNICK J. A., EGDAHL R. H. Ribonucleic acid in "transformation" of lymphoid cells. Science. 1962 Sep 21;137(3534):976–977. doi: 10.1126/science.137.3534.976. [DOI] [PubMed] [Google Scholar]
- NIU M. C., CORDOVA C. C., NIU L. C. Ribonucleic acid-induced changes in mammalian cells. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1689–1700. doi: 10.1073/pnas.47.10.1689. [DOI] [PMC free article] [PubMed] [Google Scholar]