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. 1962 Oct 1;116(4):423–432. doi: 10.1084/jem.116.4.423

THE INTRACELLULAR DISTRIBUTION OF GAMMA GLOBULIN IN A MOUSE PLASMA CELL TUMOR (X5563) AS REVEALED BY FLUORESCENCE AND ELECTRON MICROSCOPY

Richard A Rifkind 1, Elliott F Osserman 1, Konrad C Hsu 1, Councilman Morgan 1
PMCID: PMC2137627  PMID: 19867216

Abstract

Ferritin- and fluorescein-conjugated antibody staining has been applied to a study of a mouse plasma cell tumor. The presence of myeloma globulin within cisternae of the endoplasmic reticulum was observed at a stage of the secretory process when the remainder of the cytoplasm was essentially free of labeled globulin. The distribution of ferritin suggested a functional heterogeneity among units of the endoplasmic reticulum. Apparently, progressive accumulation of globulin results in distension of the endoplasmic reticulum and, occasionally, in the appearance of considerable quantities of this secretory protein in the extracisternal cytoplasmic matrix. Participation of the Golgi apparatus in the packaging and release of small quantitites of globulin seems likely. In addition, however, fragmentation of the peripheral cytoplasm with rupture of distended ergastoplasmic vesicles appeared to be another pathway whereby globulin is secreted.

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Selected References

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  1. ASKONAS B. A., WHITE R. G. Sites of antibody production in the guinea-pig; the relation between in vitro synthesis of anti-ovalbumin and gamma-globulin and distribution of antibody-containing plasma cells. Br J Exp Pathol. 1956 Feb;37(1):61–74. [PMC free article] [PubMed] [Google Scholar]
  2. BERN H. A., NISHIOKA R. S., HAGADORN I. R. Association of elementary neurosecretory granules with the Golgi complex. J Ultrastruct Res. 1961 Aug;5:311–320. doi: 10.1016/s0022-5320(61)80008-7. [DOI] [PubMed] [Google Scholar]
  3. BESSIS M. C. Ultrastructure of lymphoid and plasma cells in realtion to globulin and antibody formation. Lab Invest. 1961 Nov-Dec;10:1040–1067. [PubMed] [Google Scholar]
  4. BRAUNSTEINER H., FELLINGER K., PAKESCH F. Demonstration of a cytoplasmic structure in plasma cells. Blood. 1953 Oct;8(10):916–922. [PubMed] [Google Scholar]
  5. BRAUNSTEINER H., PAKESCH F. Electron microscopy and the functional significance of a new cellular structure in plasmocytes: a review. Blood. 1955 Jun;10(6):650–654. [PubMed] [Google Scholar]
  6. CARO L. G. Electron microscopic radioautography of thin sections: the Golgi zone as a site of protein concentration in pancreatic acinar cells. J Biophys Biochem Cytol. 1961 May;10:37–45. doi: 10.1083/jcb.10.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COONS A. H., LEDUC E. H., CONNOLLY J. M. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955 Jul 1;102(1):49–60. doi: 10.1084/jem.102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DALTON A. J., POTTER M., MERWIN R. M. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse. J Natl Cancer Inst. 1961 May;26:1221–1267. [PubMed] [Google Scholar]
  9. FAHEY J. L. Immunochemical studies of twenty mouse myeloma proteins: evidence for two groups of proteins similar to gamma and beta-2A globulins in man. J Exp Med. 1961 Sep 1;114:385–398. doi: 10.1084/jem.114.3.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FARQUHAR M. G., RINEHART J. F. Electron microscopic studies of the anterior pituitary gland of castrate rats. Endocrinology. 1954 May;54(5):516–541. doi: 10.1210/endo-54-5-516. [DOI] [PubMed] [Google Scholar]
  11. FARQUHAR M. G., WELLINGS S. R. Electron microscopic evidence suggesting secretory granule formation within the Golgi apparatus. J Biophys Biochem Cytol. 1957 Mar 25;3(2):319–322. doi: 10.1083/jcb.3.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FAWCETT D. W. The membranes of the cytoplasm. Lab Invest. 1961 Nov-Dec;10:1162–1188. [PubMed] [Google Scholar]
  13. FELDMAN M., ELSON D., GLOBERSON A. Antibodies in ribonucleoproteins. Nature. 1960 Jan 30;185:317–319. doi: 10.1038/185317a0. [DOI] [PubMed] [Google Scholar]
  14. FERREIRA D. L'ultrastructure des cellules du pancréas endocrine chez l'embryon et le rat nouveau-né. J Ultrastruct Res. 1957 Nov;1(1):14–25. doi: 10.1016/s0022-5320(57)80009-4. [DOI] [PubMed] [Google Scholar]
  15. HAGUENAU F., BERNHARD W. L'appareil de Golgi dans les cellules normales et cancéreuses de vertébrés; rappel historique et étude au microscope électronique. Arch Anat Microsc Morphol Exp. 1955;44(1):27–55. [PubMed] [Google Scholar]
  16. HERMAN L. An electron microscope study of the salamander thyroid during hormonal stimulation. J Biophys Biochem Cytol. 1960 Feb;7:143–150. doi: 10.1083/jcb.7.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HERMAN L., FITZGERALD P. J. The fine structure of the Golgi body following thyroid stimulation and pancreatic regeneration. Trans N Y Acad Sci. 1961 Feb;23:332–345. doi: 10.1111/j.2164-0947.1961.tb01360.x. [DOI] [PubMed] [Google Scholar]
  18. HOWATSON A. F., McCULLOCH E. A. Virus-like bodies in a transplantable mouse plasma cell tumour. Nature. 1958 Apr 26;181(4617):1213–1213. doi: 10.1038/1811213b0. [DOI] [PubMed] [Google Scholar]
  19. Kern M., Helmreich E., Eisen H. N. A DEMONSTRATION OF ANTIBODY ACTIVITY ON MICROSOMES. Proc Natl Acad Sci U S A. 1959 Jun;45(6):862–867. doi: 10.1073/pnas.45.6.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. NATHANS D., FAHEY J. L., POTTER M. The formation of myeloma protein by a mouse plasma cell tumor. J Exp Med. 1958 Jul 1;108(1):121–130. doi: 10.1084/jem.108.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ORTEGA L. G., MELLORS R. C. Cellular sites of formation of gamma globulin. J Exp Med. 1957 Nov 1;106(5):627–640. doi: 10.1084/jem.106.5.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PALADE G. E., SIEKEVITZ P. Pancreatic microsomes; an integrated morphological and biochemical study. J Biophys Biochem Cytol. 1956 Nov 25;2(6):671–690. doi: 10.1083/jcb.2.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. PALADE G. E. Studies on the endoplasmic reticulum. II. Simple dispositions in cells in situ. J Biophys Biochem Cytol. 1955 Nov 25;1(6):567–582. doi: 10.1083/jcb.1.6.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. PARSONS D. F., DARDEN E. B., Jr, LINDSLEY D. L., PRATT G. T. Electron microscopy of plasma-cell tumors of the mouse. I. MPC-1 and X5563 tumors. J Biophys Biochem Cytol. 1961 Feb;9:353–368. doi: 10.1083/jcb.9.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. POTTER M., KUFF E. L. Myeloma globulins of plasma-cell neoplasms in inbred mice. I. Immunoelectrophoresis of serum, with rabbit antibodies prepared against microsome fractions of the neoplasms. J Natl Cancer Inst. 1961 May;26:1109–1137. [PubMed] [Google Scholar]
  27. RIFKIND R. A., HSU K. C., MORGAN C., SEEGAL B. C., KNOX A. W., ROSE H. M. Use of ferritin-conjugated antibody to localize antigen by electron microscopy. Nature. 1960 Sep 24;187:1094–1095. doi: 10.1038/1871094a0. [DOI] [PubMed] [Google Scholar]
  28. SHELDON H., KIMBALL F. B. Studies on cartilage. III. The occurrence of collagen within vacuoles of the golgi apparatus. J Cell Biol. 1962 Mar;12:599–613. doi: 10.1083/jcb.12.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Biophys Biochem Cytol. 1960 Jul;7:619–630. doi: 10.1083/jcb.7.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SINGER S. J. Preparation of an electron-dense antibody conjugate. Nature. 1959 May 30;183(4674):1523–1524. doi: 10.1038/1831523a0. [DOI] [PubMed] [Google Scholar]
  31. WELLENSIEK H. J. Zur submikroskopischen Morphologie von Plasmazellen mit Russelschen Korperchen und Eiweisskristallen. Beitr Pathol Anat. 1957;118(2):173–202. [PubMed] [Google Scholar]
  32. WELLINGS S. R., DEOME K. B. Milk protein droplet formation in the Golgi apparatus of the C3H/Crgl mouse mammary epithelial cells. J Biophys Biochem Cytol. 1961 Feb;9:479–485. doi: 10.1083/jcb.9.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. WISSIG S. L. The anatomy of secretion in the follicular cells of the thyroid gland. I. The fine structure of the gland in the normal rat. J Biophys Biochem Cytol. 1960 Jun;7:419–432. doi: 10.1083/jcb.7.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]

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