Abstract
The influence of estradiol upon incorporation of isotopes into the sulfated acid mucopolysaccharides of connective tissues of the rat has been studied. Administration of the hormone for 3 weeks significantly reduces the in vitro incorporation of S35-sulfate into cartilage and thoracic aorta without significantly altering the amount of hydrolyzable sulfate in these tissues. The reduction of sulfate incorporation into these tissues by estradiol represents reduced synthesis since the hormone reduces incorporation of sulfate into the acid mucopolysaccharides of the tissue to the same extent and since reduction in incorporation of C14-glucose also parallels S35-sulfate incorporation into acid mucopolysaccharides. The rate of synthesis of sulfated acid mucopolysaccharides is greater in thoracic aorta than abdominal aorta or cartilage. Since the amount of hydrolyzable sulfate is the same in these two portions of aorta, the turnover is also more rapid in the thoracic aorta.
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Selected References
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- CHRISTIE R. W., DAHL L. K. Dissimilarity in oxygen consumption between the thoracic and abdominal aorta in rats. J Exp Med. 1957 Sep 1;106(3):357–364. doi: 10.1084/jem.106.3.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EINBINDER J., SCHUBERT M. Separation of chondroitin sulfate from cartilage. J Biol Chem. 1950 Aug;185(2):725–730. [PubMed] [Google Scholar]
- GARDELL S. Determination of hexosamines. Methods Biochem Anal. 1958;6:289–317. [PubMed] [Google Scholar]
- KERBY G. P. The excretion of glucuronic acid and of acid mucopolysaccharides in normal human urine. J Clin Invest. 1954 Aug;33(8):1168–1174. doi: 10.1172/JCI102989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAYTON L. L., FRANKEL D. R., SCAPA S. Quantitative differential fixation of sulfate by tissues maintained in vitro; sulfate fixation as a function of age for embryonic tissues. Cancer. 1950 Jul;3(4):725–734. doi: 10.1002/1097-0142(1950)3:4<725::aid-cncr2820030418>3.0.co;2-7. [DOI] [PubMed] [Google Scholar]
- MOSKOWITZ M. S., MOSKOWITZ A. A., BRADFORD W. L., Jr, WISSLER R. W. Changes in serum lipids and coronary arteries of the rat in response to estrogens. AMA Arch Pathol. 1956 Mar;61(3):245–263. [PubMed] [Google Scholar]
- PRIEST R. E., KOPLITZ R. M., BENDITT E. P. Estradiol reduces incorporation of radioactive sulfate into cartilage and aortas of rats. J Exp Med. 1960 Jul 1;112:225–236. doi: 10.1084/jem.112.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALMON W. D., Jr, DAUGHADAY W. H. The importance of amino acids as dialyzable components of rat serum which promote sulfate uptake by cartilage from hypophysectomized rats in vitro. J Lab Clin Med. 1958 Feb;51(2):107–173. [PubMed] [Google Scholar]
- SALMON W. D., Jr Importance of amino acids in the actions of insulin and serum sulfation factor to stimulate sulfate uptake by cartilage from hypophysectomized rats. J Lab Clin Med. 1960 Nov;56:673–681. [PubMed] [Google Scholar]