Abstract
Lymph node cells were obtained from an inbred strain of Lewis rats injected with guinea pig cord tissue in Freund's adjuvant. These cells, when added to tissue culture monolayers of puppy brain, aggregated on or around the glial elements. This reaction, called contactual agglutination, was followed by the specific destruction of glial cells, leaving cultures consisting only of fibroblasts. No such reaction was noted when lymph node cells obtained either from normal rats or those injected with adjuvant alone were used. Absorption of serum obtained from rats injected with guinea pig cord tissue by non-sensitized lymph node cells made them reactive in brain tissue culture. The contactual agglutination test seems to provide an opportunity for investigation of sensitization reaction in tissue culture systems.
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Selected References
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- COSTERO I., POMERAT C. M. Tissue cultures of cat cerebellum. Am J Anat. 1956 Sep;99(2):211–247. doi: 10.1002/aja.1000990203. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
- FERNANDES M. V., POMERAT C. M. Cytopathogenic effects of rabies virus on nervous tissue in vitro. Z Zellforsch Mikrosk Anat. 1961;53:431–437. doi: 10.1007/BF00339058. [DOI] [PubMed] [Google Scholar]
- FERNANDES M. V. The development of a human amnion strain of cells. Tex Rep Biol Med. 1958;16(1):48–58. [PubMed] [Google Scholar]
- GOVAERTS A. Cellular antibodies in kidney homotransplantation. J Immunol. 1960 Nov;85:516–522. [PubMed] [Google Scholar]
- HILD W. Myelogenesis in cultures of mammalian central nervous tissue. Z Zellforsch Mikrosk Anat. 1957;46(1):71–95. doi: 10.1007/BF01106147. [DOI] [PubMed] [Google Scholar]
- KELUS A., GURNER B. W., COOMBS R. R. Blood group antigens on HeLa cells shown by mixed agglutination. Immunology. 1959 Jul;2:262–267. [PMC free article] [PubMed] [Google Scholar]
- KOPROWSKI H. The role of hyperergy in measles encephalitis. Am J Dis Child. 1962 Mar;103:273–278. doi: 10.1001/archpedi.1962.02080020285019. [DOI] [PubMed] [Google Scholar]
- PATERSON P. Y. Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med. 1960 Jan 1;111:119–136. doi: 10.1084/jem.111.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSENAU W., MOON H. D. Lysis of homologous cells by sensitized lymphocytes in tissue culture. J Natl Cancer Inst. 1961 Aug;27:471–483. [PubMed] [Google Scholar]
- SHELOKOV A., VOGEL J. E., CHI L. Hemadsorption (adsorption-hemagglutination) test for viral agents in tissue culture with special reference to influenza. Proc Soc Exp Biol Med. 1958 Apr;97(4):802–809. doi: 10.3181/00379727-97-23884. [DOI] [PubMed] [Google Scholar]