Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1963 May 1;117(5):717–733. doi: 10.1084/jem.117.5.717

IN VITRO STUDIES OF ULCERATIVE COLITIS

II. CYTOTOXIC ACTION OF WHITE BLOOD CELLS FROM PATIENTS ON HUMAN FETAL COLON CELLS

Peter Perlmann 1, Ove Broberger 1
PMCID: PMC2137644  PMID: 13942482

Abstract

Freshly isolated fetal human colon cells were labeled with 32P-orthophosphate or 14C-amino acids and exposed to white blood cells from children with ulcerative colitis or from healthy controls. Exposure of the colon cells to patients' white cells led to a rapid isotope release, significantly higher than that obtained with normal white cells. After 150 minutes of incubation, 75 per cent of the total isotope present was found in the media of the colitis samples but only 40 per cent in those of the controls. Consistent results were obtained with white blood cells from 14 patients and 18 healthy individuals. Similar results were obtained with either fresh white cells or with white cells aged for 12 to 18 hours and consisting to 60 to 70 per cent of lymphocytes and to 20 to 30 per cent of large mononuclear cells. No specific cytotoxic activity could be conferred onto normal white cells by pretreating them with patients' serum containing antibodies against colon antigen. The cytotoxic action of the patients' white cells was immunologically specific, since no difference from the controls was found in the isotope release when cells from other organs or animals were similarly treated. Preliminary experiments suggested that the patients' white cells could be desensitized by pretreating them with colon extract. For obtaining a significant cytotoxic effect of the patients' white cells, the presence of 10 to 20 per cent of fresh guinea pig or human serum in the incubation medium was required.

Full Text

The Full Text of this article is available as a PDF (911.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOS D. B. Possible relationships between the cytotoxic effects of isoantibody and host cell function. Ann N Y Acad Sci. 1960 May 31;87:273–292. doi: 10.1111/j.1749-6632.1960.tb23201.x. [DOI] [PubMed] [Google Scholar]
  2. BERRIAN J. H., BRENT L. Cell-bound antibodies in transplantation immunity. Ann N Y Acad Sci. 1958 Oct 7;73(3):654–662. doi: 10.1111/j.1749-6632.1959.tb40841.x. [DOI] [PubMed] [Google Scholar]
  3. BILLINGHAM R. E., SILVERS W. K., WILSON D. B. Adoptive transfer of transplantation immunity by means of blood-borne cells. Lancet. 1962 Mar 10;1(7228):512–515. doi: 10.1016/s0140-6736(62)91484-8. [DOI] [PubMed] [Google Scholar]
  4. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  5. BRENT L. Tissue transplantation immunity. Prog Allergy. 1958;5:271–348. [PubMed] [Google Scholar]
  6. BROBERGER O., PERLMANN P. Autoantibodies in human ulcerative colitis. J Exp Med. 1959 Nov 1;110:657–674. doi: 10.1084/jem.110.5.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BROBERGER O., PERLMANN P. Demonstration of an epithelial antigen in colon by means of fluorescent antibodies from children with ulcerative colitis. J Exp Med. 1962 Jan 1;115:13–26. doi: 10.1084/jem.115.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BROBERGER O., PERLMANN P. In vitro studies of ulcerative colitis. I. Reactions of patients' serum with human fetal colon cells in tissue cultures. J Exp Med. 1963 May 1;117:705–716. doi: 10.1084/jem.117.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  10. FAVOUR C. B. Cell injury in allergic inflammation. Int Arch Allergy Appl Immunol. 1957;10(4):193–224. doi: 10.1159/000228377. [DOI] [PubMed] [Google Scholar]
  11. GOVAERTS A. Cellular antibodies in kidney homotransplantation. J Immunol. 1960 Nov;85:516–522. [PubMed] [Google Scholar]
  12. GREEN H., BARROW P., GOLDBERG B. Effect of antibody and complement on permeability control in ascites tumor cells and erythrocytes. J Exp Med. 1959 Nov 1;110:699–713. doi: 10.1084/jem.110.5.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GREEN H., GOLDBERG B. The action of antibody and complement on mammalian cells. Ann N Y Acad Sci. 1960 May 31;87:352–362. doi: 10.1111/j.1749-6632.1960.tb23205.x. [DOI] [PubMed] [Google Scholar]
  14. HESS E. V., ASHWORTH C. T., ZIFF M. Transfer of an autoimmune nephrosis in the rat by means of lymph node cells. J Exp Med. 1962 Feb 1;115:421–438. doi: 10.1084/jem.115.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HOLMBERG B. On the permeability to lissamine green and other dyes in the course of cell injury and cell death. Exp Cell Res. 1961 Jan;22:406–414. doi: 10.1016/0014-4827(61)90118-5. [DOI] [PubMed] [Google Scholar]
  16. LAGERCRANTZ R., WINBERG J., ZETTERSTROM R. Extra-colonic manifestations in chronic ulcerative colitis. Acta Paediatr. 1958 Nov;47(6):675–687. doi: 10.1111/j.1651-2227.1958.tb07683.x. [DOI] [PubMed] [Google Scholar]
  17. LAWRENCE H. S. Homograft sensitivity. An expression of the immunologic origins and consequences of individuality. Physiol Rev. 1959 Oct;39:811–859. doi: 10.1152/physrev.1959.39.4.811. [DOI] [PubMed] [Google Scholar]
  18. LEVINE S. Effect of manipulation on 32P loss from tissue culture cells. Exp Cell Res. 1960 Mar;19:220–227. doi: 10.1016/0014-4827(60)90003-3. [DOI] [PubMed] [Google Scholar]
  19. METAXAS M. N., METAXAS-BUEHLER M. Studies on the cellular transfer of tuberculin sensitivity in the guinea pig. J Immunol. 1955 Nov;75(5):333–347. [PubMed] [Google Scholar]
  20. NAJARIAN J. S., FELDMAN J. D. Passive transfer of tuberculin sensitivity by tritiated thymidine-labeled lymphoid cells. J Exp Med. 1961 Nov 1;114:779–790. doi: 10.1084/jem.114.5.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PATERSON P. Y. Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med. 1960 Jan 1;111:119–136. doi: 10.1084/jem.111.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PRIEST R. J., REBUCK J. W., HAVEY G. T. A new qualitative defect of leukocyte function in ulcerative colitis. Gastroenterology. 1960 May;38:715–720. [PubMed] [Google Scholar]
  23. ROSENAU W., MOON H. D. Lysis of homologous cells by sensitized lymphocytes in tissue culture. J Natl Cancer Inst. 1961 Aug;27:471–483. [PubMed] [Google Scholar]
  24. SCOTHORNE R. J., NAGY I. In vitro studies of the interaction of lymph node and homologous tissue. Ann N Y Acad Sci. 1960 May 31;87:149–155. doi: 10.1111/j.1749-6632.1960.tb23187.x. [DOI] [PubMed] [Google Scholar]
  25. TROWELL O. A. The culture of mature organs in a synthetic medium. Exp Cell Res. 1959 Jan;16(1):118–147. doi: 10.1016/0014-4827(59)90201-0. [DOI] [PubMed] [Google Scholar]
  26. WAKSMAN B. H. Cell lysis and related phenomena in hypersensitive reactions, including immunohematologic diseases. Prog Allergy. 1958;5:349–458. [PubMed] [Google Scholar]
  27. WAKSMAN B. H., MATOLTSY M. The effect of tuberculin on peritoneal exudate cells of sensitized guinea pigs in surviving cell culture. J Immunol. 1958 Sep;81(3):220–234. [PubMed] [Google Scholar]
  28. WINN H. J. Immune mechanisms in homotransplantation. I. The role of serum antibody and complement in the neutralization of lymphoma cells. J Immunol. 1960 May;84:530–538. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES