Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Sep;169(9):4410–4414. doi: 10.1128/jb.169.9.4410-4414.1987

Physical map of the Rhodobacter sphaeroides bacteriophage phi RsG1 genome and location of the prophage on the host chromosome.

M Duchrow, F Giffhorn
PMCID: PMC213765  PMID: 3040695

Abstract

We constructed a physical map of the 50-kilobase-pair (kb) DNA of the temperate Rhodobacter sphaeroides bacteriophage phi RsG1, with the relative positions of the cleavage sites for the nine restriction endonucleases KpnI, HindIII, XbaI, ClaI, BclI, EcoRV, EcoRI, BglII, and BamHI indicated. Using biotinylated phi RsG1 DNA as a probe in hybridization studies, we detected homologies with virus DNA and fragments of restriction endonuclease-digested host chromosomal DNA but not with plasmid DNA. This indicates that the prophage is integrated into the host chromosome. In addition, the use of specific probes such as the 10.4-kb BglII A fragment and the 2.65-kb BamHI H fragment allowed the determination of the position of phage attachment site (attP).

Full text

PDF
4410

Images in this article

4412Image
on p.4412
4413Image
on p.4413
4413Image
on p.4413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Cleary P., Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968 Nov;61(3):956–962. doi: 10.1073/pnas.61.3.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chan V. T., Fleming K. A., McGee J. O. Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes. Nucleic Acids Res. 1985 Nov 25;13(22):8083–8091. doi: 10.1093/nar/13.22.8083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chater K. F., Bruton C. J., Springer W., Suarez J. E. Dispensable sequences and packaging constraints of DNA from the Streptomyces temperate phage phi C31. Gene. 1981 Nov;15(2-3):249–256. doi: 10.1016/0378-1119(81)90134-7. [DOI] [PubMed] [Google Scholar]
  4. Donohue T. J., Chory J., Goldsand T. E., Lynn S. P., Kaplan S. Structure and physical map of Rhodopseudomonas sphaeroides bacteriophage RS1 DNA. J Virol. 1985 Jul;55(1):147–157. doi: 10.1128/jvi.55.1.147-157.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Errington J. Efficient Bacillus subtilis cloning system using bacteriophage vector phi 105J9. J Gen Microbiol. 1984 Oct;130(10):2615–2628. doi: 10.1099/00221287-130-10-2615. [DOI] [PubMed] [Google Scholar]
  6. Fornari C. S., Watkins M., Kaplan S. Plasmid distribution and analyses in Rhodopseudomonas sphaeroides. Plasmid. 1984 Jan;11(1):39–47. doi: 10.1016/0147-619x(84)90005-2. [DOI] [PubMed] [Google Scholar]
  7. KELLY B. Localization of P2 prophage in two strains of Escherichia coli. Virology. 1963 Jan;19:32–39. doi: 10.1016/0042-6822(63)90021-7. [DOI] [PubMed] [Google Scholar]
  8. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGadey J. A tetrazolium method for non-specific alkaline phosphatase. Histochemie. 1970;23(2):180–184. doi: 10.1007/BF00305851. [DOI] [PubMed] [Google Scholar]
  10. Pemberton J. M., Tucker W. T. Naturally occurring viral R plasmid with a circular supercoiled genome in the extracellular state. Nature. 1977 Mar 3;266(5597):50–51. doi: 10.1038/266050a0. [DOI] [PubMed] [Google Scholar]
  11. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  12. Schaffer H. E., Sederoff R. R. Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981 Jul 15;115(1):113–122. doi: 10.1016/0003-2697(81)90533-9. [DOI] [PubMed] [Google Scholar]
  13. Shub D. A., Casna N. J. Bacteriophage T4, a new vector for the expression of cloned genes. Gene. 1985;37(1-3):31–36. doi: 10.1016/0378-1119(85)90254-9. [DOI] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Tucker W. T., Pemberton J. M. Viral R plasmid Rphi6P: properties of the penicillinase plasmid prophage and the supercoiled, circular encapsidated genome. J Bacteriol. 1978 Jul;135(1):207–214. doi: 10.1128/jb.135.1.207-214.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES