Abstract
Immunization of mice or guinea pigs with BCG rendered all or most of the histiocytes of these animals resistant to necrotization by virulent H37Rv; this cellular resistance was mediated by immune serum. Immune mouse histiocytes (from BCG-immunized animals) were able to induce cellular resistance in normal homologous and heterologous (rabbit) animal species; mouse histiocytic ribosomes were also tested in the homologous species and found to be active. Immune guinea pig histiocytes (from BCG-immunized guinea pigs) were ineffective in transferring cellular resistance to either homologous or heterologous (mouse and rabbit) animal species. Immune rabbit histiocytes were capable of inducing cellular resistance in mice and guinea pigs; rabbit histiocytic ribosomes were also tested in normal mice and found to be active in induction of cellular resistance. Recipient guinea pig histiocytes (from guinea pigs inoculated with immune rabbit histiocytes) were capable of inducing cellular resistance in normal guinea pigs and rabbits. Cultivation of lysed immune histiocytes of all three animal species on glycerol-blood agar medium failed to reveal any viable BCG; this provided one additional bit of evidence against the idea that induction of cellular resistance is due to viable bacilli.
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Selected References
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- ELBERG S. S., FONG J., SCHNEIDER P. Studies on tubercle bacillus-monocyte relationship. I. Quantitative analysis of effect of serum of animals vaccinated with BCG upon bacterium-monocyte system. J Exp Med. 1956 Oct 1;104(4):455–465. doi: 10.1084/jem.104.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FONG J., CHIN D., ELBERG S. S. STUDIES OF TUBERCLE BACILLUS-HISTIOCYTE RELATIONSHIPS. VI. INDUCTION OF CELLULAR RESISTANCE BY RIBOSOMES AND RIBOSOMAL RNA. J Exp Med. 1963 Sep 1;118:371–386. doi: 10.1084/jem.118.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FONG J., CHIN D., ELBERG S. S. Studies on tubercle bacillus-histiocyte relationship. V. Passive transfer of cellular resistance. J Exp Med. 1962 Mar 1;115:475–489. doi: 10.1084/jem.115.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LITTLEFIELD J. W., KELLER E. B., GROSS J., ZAMECNIK P. C. Studies on cytoplasmic ribonucleoprotein particles from the liver of the rat. J Biol Chem. 1955 Nov;217(1):111–123. [PubMed] [Google Scholar]
- MACKANESS G. B. The action of drugs on intracellular tubercle bacilli. J Pathol Bacteriol. 1952 Jul;64(3):429–446. doi: 10.1002/path.1700640302. [DOI] [PubMed] [Google Scholar]
- NIU M. C., CORDOVA C. C., NIU L. C. Ribonucleic acid-induced changes in mammalian cells. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1689–1700. doi: 10.1073/pnas.47.10.1689. [DOI] [PMC free article] [PubMed] [Google Scholar]