Abstract
The initial morphologic alteration in rabbit polymorphonuclear leucocytes exposed to streptolysin is rapid and extensive lysis of cytoplasmic granules. The granules appear to rupture directly into the cell sap. Within a few minutes following degranulation, the leucocyte rounds up, filamentous processes appear on the cell membrane, the cytoplasm liquefies, and finally the nuclear lobes swell and fuse. Streptolysin O causes these changes in intact leucocytes when added in concentrations only slightly higher than those required for release of hydrolases from isolated liver lysosomes, and furthermore exerts its action on granulocytes promptly. On the other hand streptolysin S acts on white cells only after a 15 to 30 minute delay, and the levels necessary to disrupt granules in leucocytes are considerably higher than those which act on lysosome suspensions. Exposure of rabbit alveolar macrophages to streptolysin O also results in lysis of granules, soon followed by alterations in the cytoplasm and membrane. The observations are in accord with the hypothesis that streptolysins penetrate the leucocyte membrane and bring about lysis of granules. Autolytic enzymes released from the granules might then be responsible for the subsequent damage seen in various other cell structures.
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Selected References
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- BERNHEIMER A. W. Formation of a bacterial toxin (streptolysin S) by resting cells. J Exp Med. 1949 Nov;90(5):373–392. doi: 10.1084/jem.90.5.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BERNHEIMER A. W., SCHWARTZ L. L. Leucocidal agents of haemolytic streptococci. J Pathol Bacteriol. 1960 Jan;79:37–46. doi: 10.1002/path.1700790105. [DOI] [PubMed] [Google Scholar]
- COHN Z. A., HIRSCH J. G. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:983–1004. doi: 10.1084/jem.112.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HIRSCH J. G. Cinemicrophotographic observations on granule lysis in polymorphonuclear leucocytes during phagocytosis. J Exp Med. 1962 Dec 1;116:827–834. doi: 10.1084/jem.116.6.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MYRVIK Q. N., LEAKE E. S., OSHIMA S. A study of macrophages and epitheloid-like cells from granulomatous (BCG-induced) lungs of rabbits. J Immunol. 1962 Nov;89:745–751. [PubMed] [Google Scholar]
- WEISSMANN G., KEISER H., BERNHEIMER A. W. STUDIES ON LYSOSOMES. III. THE EFFECTS OF STREPTOLYSINS O AND S ON THE RELEASE OF ACID HYDROLASES FROM A GRANULAR FRACTION OF RABBIT LIVER. J Exp Med. 1963 Aug 1;118:205–222. doi: 10.1084/jem.118.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOODIN A. M. The effect of staphylococcal leucocidin on the leucocyte. Biochem J. 1961 Sep;80:562–572. doi: 10.1042/bj0800562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOODIN A. M. The extrusion of protein from the rabbit polymorphonuclear leucocyte treated with staphylococcal leucocidin. Biochem J. 1962 Jan;82:9–15. doi: 10.1042/bj0820009. [DOI] [PMC free article] [PubMed] [Google Scholar]