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. 1964 Aug 1;120(2):129–148. doi: 10.1084/jem.120.2.129

THE SUSCEPTIBILITY OF CHICK EMBRYO SKIN ORGAN CULTURES TO INFLUENZA VIRUS FOLLOWING EXCESS VITAMIN A

J S Huang 1, F B Bang 1
PMCID: PMC2137735  PMID: 14206436

Abstract

The conversion of chick embryonic epidermis to mucous epithelium by excess vitamin A in organ culture as reported by Fell and Mellanby (5) was shown to be accompanied by a corresponding change of susceptibility to influenza and vaccinia viruses. Untreated epidermis of 10- to 12-day chick embryos supported the growth of influenza (PR8) virus in organ cultures and a maximum infectivity (EID50) titer was reached 2 to 3 days after infection. At the same time) the epidermis showed squamous keratinization, beginning about the 4th day of cultivation. Addition of excess vitamin A (40 µg per ml) to the skin organ culture induced the following changes: (a) mucous metaplasia of the epidermis which was usually first evident after 4 to 5 days in the vitamin A medium, (b) increase in the daily and maximum yield of influenza virus, if the epidermis had been grown for 4 or more days in the vitamin A medium before infection took place, and (c) decrease in the production of vaccinia virus under similar conditions. The maximum yield of both viruses remained unchanged, however, if excess vitamin A was introduced to the organ culture at the time of virus inoculation. The magnitude of increase in the yield of influenza virus in this organ culture system was found to be proportionally related to the concentration of vitamin A added 4 or more days before inoculation of this virus. Increasing doses of vitamin A however, had no effect on the short-term growth of influenza virus in tissue cultures of chorio-allantoic membrane. Observation on the early period (2 to 12 hours) of influenza virus growth initiated in the 4-day organ cultures of chick embryonic skin showed no significant difference in virus production between the normal and the vitamin A medium groups. The change of virus specificity apparently is not due to the presence of excess vitamin A per se, but appears to be related to the change of differentiation produced in the organ culture system.

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Selected References

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  1. BANG F. B., LUTTRELL C. N. Factors in the pathogenesis of virus diseases. Adv Virus Res. 1961;8:199–244. doi: 10.1016/s0065-3527(08)60686-7. [DOI] [PubMed] [Google Scholar]
  2. BANG F. B., NIVEN J. S. A study of infection in organised tissue cultures. Br J Exp Pathol. 1958 Jun;39(3):317–322. [PMC free article] [PubMed] [Google Scholar]
  3. BLOUGH H. A. The effect of vitamin A alcohol on the morphology of myxoviruses. I. The production and comparison of artificially produced filamentous virus. Virology. 1963 Mar;19:349–358. doi: 10.1016/0042-6822(63)90074-6. [DOI] [PubMed] [Google Scholar]
  4. DINGLE J. T. Studies on the mode of action of excess of vitamin A. 3. Release of a bound protease by the action of vitamin A. Biochem J. 1961 Jun;79:509–512. doi: 10.1042/bj0790509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FELL H. B., MELLANBY E. Metaplasia produced in cultures of chick ectoderm by high vitamin A. J Physiol. 1953 Mar;119(4):470–488. doi: 10.1113/jphysiol.1953.sp004860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FELL H. B., MELLANBY E., PELC S. R. Influence of excess vitamin A on the sulphate metabolism of bone rudiments grown in vitro. J Physiol. 1956 Oct 29;134(1):179–188. doi: 10.1113/jphysiol.1956.sp005633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FELL H. B., MELLANBY E., PELC S. R. Influence of excess vitamin A on the sulphate metabolism of chick ectoderm grown in vitro. Br Med J. 1954 Sep 11;2(4888):611–611. doi: 10.1136/bmj.2.4888.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FELL H. B., THOMAS L. Comparison of the effects of papain and vitamin A on cartilage. II. The effects on organ cultures of embryonic skeletal tissue. J Exp Med. 1960 May 1;111:719–744. doi: 10.1084/jem.111.5.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FELL H. B. The effect of excess vitamin A on cultures of embryonic chicken skin explanted at different stages of differentiation. Proc R Soc Lond B Biol Sci. 1956 Mar 26;146(923):242–256. doi: 10.1098/rspb.1957.0008. [DOI] [PubMed] [Google Scholar]
  10. Fell H. B., Robison R. The growth, development and phosphatase activity of embryonic avian femora and limb-buds cultivated in vitro. Biochem J. 1929;23(4):767–784.5. doi: 10.1042/bj0230767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LIU O. C., HENLE W. Studies on host-virus interactions in the chick embryo-influenza virus system. IV. The role of inhibitors of hemagglutination in the evaluation of viral multiplication. J Exp Med. 1951 Oct;94(4):269–289. doi: 10.1084/jem.94.4.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NOYES W. F., MELLORS R. C. Fluorescent antibody detection of the antigens of the Shope papilloma virus in papillomas of the wild and domestic rabbit. J Exp Med. 1957 Oct 1;106(4):555–562. doi: 10.1084/jem.106.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NOYES W. F. Studies on the Shope rabbit papilloma virus. II. The location of infective virus in papillomas of the cottontail rabbit. J Exp Med. 1959 Apr 1;109(4):423–428. doi: 10.1084/jem.109.4.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHLESINGER R. W., KARR H. V. Influenza virus and its mucoprotein substrate in the chorioallantoic membrane of the chick embryo. II. Stepwise inactivation of substrate and its relation to the mode of viral multiplication. J Exp Med. 1956 Mar 1;103(3):333–349. doi: 10.1084/jem.103.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SHAFFER B. M. The culture of organs from the embryonic chick on cellulose acetate fabric. Exp Cell Res. 1956 Aug;11(1):244–248. doi: 10.1016/0014-4827(56)90217-8. [DOI] [PubMed] [Google Scholar]
  16. THOMAS L., McCLUSKEY R. T., POTTER J. L., WEISSMANN G. Comparison of the effects of papain n vitamin A on cartilage. I. The effects in rabbits. J Exp Med. 1960 May 1;111:705–718. doi: 10.1084/jem.111.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WOLF G., VARANDANI P. T. Studies on the function of vitamin A in mucopolysaccharide biosynthesis. Biochim Biophys Acta. 1960 Oct 7;43:501–512. doi: 10.1016/0006-3002(60)90472-8. [DOI] [PubMed] [Google Scholar]

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