Abstract
From Mycobacterium tuberculosis, a serologically specific polysaccharide was purified. It contained mannose, arabinose, and a trace of glucose. It was not active in hemagglutination (HA) reaction. Partial acylation of the polysaccharide was carried out with palmitoylchloride, and the synthetic lipopolysaccharide contained 15.1 per cent of esterified palmitic acid. The synthetic lipopolysaccharide had hemagglutination activity and 0.5 µg was able to sensitize 1 ml of 2 per cent erythrocyte suspension; it had the same activity as the natural antigenic lipopolysaccharide. The same antibody in the antiserum was involved in the hemagglutination reaction with both the synthetic and natural lipopolysaccharides. The sensitizing activity of the synthetic lipopolysaccharide was completely neutralized by cholesterol. Model experiments showed that the lipid moiety of hemagglutination antigen was essential for the sensitization of erythrocytes. The mechanism of the hemagglutination reaction is discussed.
Full Text
The Full Text of this article is available as a PDF (471.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GORZYNSKI E. A., LUDERITZ O., NETER E., WESTPHAL O. The bacterial hemagglutination test for the demonstration of antibodies to Enterobacteriaceae. Ann N Y Acad Sci. 1956 Aug 10;66(1):141–156. doi: 10.1111/j.1749-6632.1956.tb40113.x. [DOI] [PubMed] [Google Scholar]
- King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MATSUMOTO R. Chemical and biological properties of a lipopolysaccharide of BCG responsible for hemagglutination reaction. Jpn J Med Sci Biol. 1960 Jun;13:139–145. doi: 10.7883/yoken1952.13.139. [DOI] [PubMed] [Google Scholar]
- MIDDLEBROOK G., DUBOS R. J. Specific serum agglutination of erythrocytes sensitized with extracts of tubercle bacilli. J Exp Med. 1948 Nov;88(5):521–528. doi: 10.1084/jem.88.5.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NETER E., COHEN E., WESTPHAL O., LUDERITZ O. The effects of proteolytic enzymes on agglutination by bacterial antibodies of lipopolysaccharide modified erythrocytes. J Immunol. 1959 Feb;82(2):85–93. [PubMed] [Google Scholar]
- SORKIN E., BOYDEN S. V. A study of antigens active in the Middlebrook-Dubos hemagglutination test present in filtrates of culture of Mycobacterium tuberculosis. J Immunol. 1955 Jul;75(1):22–27. [PubMed] [Google Scholar]
- TSUMITA T., MATSUMOTO R., MIZUNO D. Chemical and biological properties of the hemagglutination antigen, a lipopolysaccharide, of Mycobacterium tuberculosis, var. hominis. Jpn J Med Sci Biol. 1960 Jun;13:131–138. doi: 10.7883/yoken1952.13.131. [DOI] [PubMed] [Google Scholar]
- TSUMITA T., MATSUMOTO R., MIZUNO D. The nature of the Middlebrook-Dubos haemagglutination antigen of Mycobacterium tuberculosis. Jpn J Med Sci Biol. 1959 Jun;12:167–170. doi: 10.7883/yoken1952.12.167. [DOI] [PubMed] [Google Scholar]
- YEMM E. W., WILLIS A. J. The estimation of carbohydrates in plant extracts by anthrone. Biochem J. 1954 Jul;57(3):508–514. doi: 10.1042/bj0570508. [DOI] [PMC free article] [PubMed] [Google Scholar]