Abstract
The toxin activity of Pasteurella pestis cells, strain "Tjiwidej," was found to be associated with two proteins. Using a disc electrophoresis technique in conjunction with mouse lethality, two toxic proteins were isolated exhibiting intraperitoneal LD 50's of less than 1.0 to 1.5 µg protein. Each produced a single characteristic precipitin band on agar gel diffusion plates. The slower migrating toxin in gel diffusion or disc electrophoresis was designated as toxin A. It was shown to be sensitive to deoxycholate and digitonin, did not accumulate in 5-fluorotryptophan treated cells, and was associated with the membrane fraction of the cell. The faster migrating toxin B, apparently is resistant to surface-active agents, and is not affected by treatment of cells with 5-fluorotryptophan. Toxin B is associated with the soluble or cytoplasmic fraction of the cell. This evidence suggested that each toxin represented a distinctly different molecular species. The possibility is discussed that toxin B is synonymous with the murine toxin previously isolated by paper curtain electrophoresis which revealed only one antigen band in the Oudin precipitin reaction.
Full Text
The Full Text of this article is available as a PDF (744.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AJL S. J., RUST J., Jr, HUNTER D., WOEBKE J., BENT D. F. Preparation of serologically homogeneous plague murine toxin and its reactions with physical, chemical and enzymatic agents. J Immunol. 1958 Jun;80(6):435–440. [PubMed] [Google Scholar]
- BROOME J. A RAPID METHOD OF DISK ELECTROPHORESIS. Nature. 1963 Jul 13;199:179–180. doi: 10.1038/199179b0. [DOI] [PubMed] [Google Scholar]
- GOODNER K., PANNELL L., BARTELL P., ROTHSTEIN E. L. Toxic end products from Pasteurella pestis. I. A comparison of lysate toxin with that obtained from the action of bile salts. J Infect Dis. 1955 Jan-Feb;96(1):82–87. doi: 10.1093/infdis/96.1.82. [DOI] [PubMed] [Google Scholar]
- KADIS S., AJL S. J., RUST J. H., Jr ACTION OF PLAGUE MURINE TOXIN ON MITOCHONDRIA FROM RESISTANT AND SUSCEPTIBLE ANIMALS. J Bacteriol. 1963 Oct;86:757–765. doi: 10.1128/jb.86.4.757-765.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIMBERG D. V., YIELDING K. L. Pyruvate kinase. Structural and functional changes induced by diethylstilbestrol and certain steroid hormones. J Biol Chem. 1962 Oct;237:3233–3239. [PubMed] [Google Scholar]
- MONTIE T. C., AJL S. J. THE ANATOMICAL DISTRIBUTION OF MURINE TOXIN IN SPHEROPLASTS OF PASTEURELLA PESTIS. J Gen Microbiol. 1964 Feb;34:249–258. doi: 10.1099/00221287-34-2-249. [DOI] [PubMed] [Google Scholar]
- OUCHTERLONY O. In vitro method for testing the toxin-producing capacity of diphtheria bacteria. Acta Pathol Microbiol Scand. 1949;26(4):516–524. doi: 10.1111/j.1699-0463.1949.tb00752.x. [DOI] [PubMed] [Google Scholar]
- OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
- SCHLESSINGER D. PROTEIN SYNTHESIS BY POLYRIBOSOMES ON PROTOPLAST MEMBRANES OF B. MEGATERIUM. J Mol Biol. 1963 Nov;7:569–582. doi: 10.1016/s0022-2836(63)80103-5. [DOI] [PubMed] [Google Scholar]
- WINSTEN S., FRIEDMAN H., SCHWARTZ E. E. LARGE-VOLUME CONTINUOUS-FLOW ELECTROPHORESIS OF SERUM PROTEINS WITH GLASS MICROBEADS. Anal Biochem. 1963 Nov;6:404–414. doi: 10.1016/0003-2697(63)90093-9. [DOI] [PubMed] [Google Scholar]
- ZELDIN M. H., WARD J. M. Acrylamide electrophoresis and protein pattern during morphogenesis in a slime mould. Nature. 1963 Apr 27;198:389–390. doi: 10.1038/198389a0. [DOI] [PubMed] [Google Scholar]