Abstract
Paracolobactrum ballerup, an organism considered completely insusceptible to the bactericidal action of the antibody-complement system, became extremely sensitive to immune serum and even to normal serum, in conjunction with complement, when cultivated at temperatures above 37°C. This conversion to serum sensitivity was associated with the loss of the organism's Vi antigen and its tendency to assume a rough state. It did not result in a genetic change in the organism, however, since reincubation of serum-sensitive P. ballerup at 37°C resulted in a restoration of its resistance. The loss of serum resistance as a result of cultivation temperatures above 37°C may be of significance as an example of the enhancement of host defense mechanisms induced by fever. Special conditions of testing indicated that P. ballerup cultured at 37°C and S. paratyphosa C, organisms considered insusceptible to serum bactericidal action, were not entirely refractory to serum. These conditions included simply a relatively low ratio of the number of test organisms to serum volume and an extended incubation period of the organisms with serum bactericidal substances, or even without these substances, prior to the initiation of bactericidal action. It is likely, therefore, that an absolute distinction between serum-sensitive and serum-resistant Gram-negative organisms does not exist, but rather that there is a very broad distribution of serum sensitivity among these organisms.
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Selected References
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- BENNETT I. L., Jr, NICASTRI A. Fever as a mechanism of resistance. Bacteriol Rev. 1960 Mar;24(1):16–34. doi: 10.1128/br.24.1.16-34.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KORNFELD L., HAMMOND C. W., MILLER C. P. The effect of irradiation on natural bactericidins of mice. J Immunol. 1960 Jan;84:77–81. [PubMed] [Google Scholar]
- LANDY M. Studies on Vi antigen. I. Relative Vi antigen content of V form cultures. Proc Soc Exp Biol Med. 1952 May;80(1):55–58. doi: 10.3181/00379727-80-19520. [DOI] [PubMed] [Google Scholar]
- MUSCHEL L. H. Bactericidal activity of normal serum against bacterial cultures. II. Activity against Eschericha coli strains. Proc Soc Exp Biol Med. 1960 Mar;103:632–636. doi: 10.3181/00379727-103-25619. [DOI] [PubMed] [Google Scholar]
- MUSCHEL L. H., CAREY W. F., BARON L. S. Formation of bacterial protoplasts by serum components. J Immunol. 1959 Jan;82(1):38–42. [PubMed] [Google Scholar]
- MUSCHEL L. H., CHAMBERLIN R. H., OSAWA E. Bactericidal activity of normal serum against bacterial cultures. I. Activity against Salmonella typhi strains. Proc Soc Exp Biol Med. 1958 Feb;97(2):376–382. doi: 10.3181/00379727-97-23748. [DOI] [PubMed] [Google Scholar]
- MUSCHEL L. H., LOWE K. M. A new complement fixation test for syphilis. J Lab Clin Med. 1955 Jul;46(1):147–154. [PubMed] [Google Scholar]
- MUSCHEL L. H. Serum bactericidal actions. Ann N Y Acad Sci. 1960 Nov 21;88:1265–1272. doi: 10.1111/j.1749-6632.1960.tb20117.x. [DOI] [PubMed] [Google Scholar]
- MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity. J Immunol. 1956 Jan;76(1):1–10. [PubMed] [Google Scholar]
- MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. III. Observations on sera obtained after T.A.B. vaccination or during typhoid fever. J Immunol. 1956 Jan;76(1):20–27. [PubMed] [Google Scholar]
- NAGINGTON J. The sensitivity of Salmonella typhi to the bactericidal action of antibody. Br J Exp Pathol. 1956 Aug;37(4):397–405. [PMC free article] [PubMed] [Google Scholar]
- NELSON R. A., Jr, MAYER M. M. Immobilization of Treponema pallidum in vitro by antibody produced in syphilitic infection. J Exp Med. 1949 Apr 1;89(4):369–393. doi: 10.1084/jem.89.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROANTREE R. J., PAPPAS N. C. The survival of strains of enteric bacilli in the blood stream as related to their sensitivity to the bactericidal effect of serum. J Clin Invest. 1960 Jan;39:82–88. doi: 10.1172/JCI104031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TREFFERS H. P., MUSCHEL L. H. The combined actions of chloramphenicol and of bactericidal antibody plus complement on Salmonella typhosa. J Exp Med. 1954 Feb;99(2):155–165. doi: 10.1084/jem.99.2.155. [DOI] [PMC free article] [PubMed] [Google Scholar]