Abstract
Multiplication of Salmonella enteritidis was inhibited in vitro by buffered suspensions of fecal material freshly removed from the large intestine of normal mice. Most effective was material obtained from cecum and transverse colon. Inhibitory activity was not impaired by sterilization by heat or filtration. From such materials were isolated acetic and butyric acids in concentrations which inhibited Salmonella in vitro. The degree of inhibitory activity of suspensions of colon content and of mixtures of the two fatty acids was conditioned by pH and favored by anaerobiosis. Effective inhibition occurred at or slightly below the pH of colon content of most normal mice as determined in situ by direct measurement. Acetic and butyric acids were isolated from anaerobic cultures of several strains of Bacteroides previously demonstrated to be one of the most numerous inhabitants of the large intestine of the normal mouse.
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Selected References
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- BOHNHOFF M., DRAKE B. L., MILLER C. P. Effect of streptomycin on susceptibility of intestinal tract to experimental Salmonella infection. Proc Soc Exp Biol Med. 1954 May;86(1):132–137. doi: 10.3181/00379727-86-21030. [DOI] [PubMed] [Google Scholar]
- BOHNHOFF M., MILLER C. P. Enhanced susceptibility to Salmonella infection in streptomycin-treated mice. J Infect Dis. 1962 Sep-Oct;111:117–127. doi: 10.1093/infdis/111.2.117. [DOI] [PubMed] [Google Scholar]
- BOHNHOFF M., MILLER C. P., MARTIN W. R. RESISTANCE OF THE MOUSE'S INTESTINAL TRACT TO EXPERIMENTAL SALMONELLA INFECTION. II. FACTORS RESPONSIBLE FOR ITS LOSS FOLLOWING STREPTOMYCIN TREATMENT. J Exp Med. 1964 Nov 1;120:817–828. doi: 10.1084/jem.120.5.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine A. S., Fellers C. R. Action of Acetic Acid on Food Spoilage Microörganisms. J Bacteriol. 1940 May;39(5):499–515. doi: 10.1128/jb.39.5.499-515.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MEYNELL G. G. Antibacterial mechanisms of the mouse gut. II. The role of Eh and volatile fatty acids in the normal gut. Br J Exp Pathol. 1963 Apr;44:209–219. [PMC free article] [PubMed] [Google Scholar]
- MEYNELL G. G., SUBBAIAH T. V. Antibacterial mechanisms of the mouse gut. I. Kinetics of infection by Salmonella typhi-murium in normal and streptomycin-treated mice studied with abortive transductants. Br J Exp Pathol. 1963 Apr;44:197–208. [PMC free article] [PubMed] [Google Scholar]
- MILLER C. P., BOHNHOFF M. CHANGES IN THE MOUSE'S ENTERIC MICROFLORA ASSOCIATED WITH ENHANCED SUSCEPTIBILITY TO SALMONELLA INFECTION FOLLOWING STREPTOMYCIN TREATMENT. J Infect Dis. 1963 Jul-Aug;113:59–66. doi: 10.1093/infdis/113.1.59. [DOI] [PubMed] [Google Scholar]
- MILLER C. P. Protective action of the normal microflora against enteric infection: an experimental study in the mouse. Med Bull (Ann Arbor) 1959 Aug;25:272–279. [PubMed] [Google Scholar]
- SIEBURTH J. M. Gastrointestinal microflora of antarctic birds. J Bacteriol. 1959 May;77(5):521–531. doi: 10.1128/jb.77.5.521-531.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sieburth J. M. ANTIBIOTIC PROPERTIES OF ACRYLIC ACID, A FACTOR IN THE GASTROINTESTINAL ANTIBIOSIS OF POLAR MARINE ANIMALS. J Bacteriol. 1961 Jul;82(1):72–79. doi: 10.1128/jb.82.1.72-79.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]