Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1964 Oct 31;120(5):691–709. doi: 10.1084/jem.120.5.691

STRUCTURAL STUDIES OF HUMAN 7S γ-GLOBULIN (G IMMUNOGLOBULIN)

FURTHER OBSERVATIONS OF A NATURALLY OCCURRING PROTEIN RELATED TO THE CRYSTALLIZABLE (FAST) FRAGMENT

E C Franklin 1
PMCID: PMC2137864  PMID: 14247713

Abstract

1. Detailed physical, chemical, and immunologic studies of a protein closely related to the Fc fragment and heavy chain of G immunoglobulin (IgG), and elaborated by a subject with a lymphoproliferative disorder are presented. 2. The protein, which has a molecular weight of 51,000, was cleaved into two half molecules by reduction and alkylation. 3. The protein has few if any of the antigenic determinants of the antigen-binding (Fab) papain fragment of IgG, and has a striking similarity in its antigenic properties to the Fc fragment. 4. Fingerprint patterns resemble those of the crystallizable (Fc) fragment, and lack several peptides found in the heavy chain. 5. These findings suggest that the Fc fragment may be a real structural unit of IgG, and raise the possibility of the existence of three different types of polypeptide chains in G immunoglobulin.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLIEUX R. E., BERNIER G. M., TOMINAGA K., PUTNAM F. W. GAMMA GLOBULIN ANTIGENIC TYPES DEFINED BY HEAVY CHAIN DETERMINANTS. Science. 1964 Jul 10;145(3628):168–170. doi: 10.1126/science.145.3628.168. [DOI] [PubMed] [Google Scholar]
  2. BERGGARD I., EDELMAN G. M. Normal counterparts to Bence-Jones proteins: free L polypeptide chains of human gamma-globulin. Proc Natl Acad Sci U S A. 1963 Mar 15;49:330–337. doi: 10.1073/pnas.49.3.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S., Porter R. R. Heterogeneity of the peptide chains of gamma-globulin. Biochem J. 1964 Feb;90(2):278–284. doi: 10.1042/bj0900278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FEINSTEIN A., GELL P. G., KELUS A. S. IMMUNOCHEMICAL ANALYSIS OF RABBIT GAMMA-G.OBULIN ALLOTYPES. Nature. 1963 Nov 16;200:653–654. doi: 10.1038/200653a0. [DOI] [PubMed] [Google Scholar]
  5. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  6. FRANKLIN E. C., KUNKEL H. G., MULLER-EBERHARD H. J. Two types of gamma-globulin differing in carbohydrate content. Proc Soc Exp Biol Med. 1956 Oct;93(1):146–150. doi: 10.3181/00379727-93-22690. [DOI] [PubMed] [Google Scholar]
  7. FRANKLIN E. C., LOWENSTEIN J., BIGELOW B., MELTZER M. HEAVY CHAIN DISEASE- A NEW DISORDER OF SERUM GAMMA-GLOBULINS : REPORT OF THE FIRST CASE. Am J Med. 1964 Sep;37:332–350. doi: 10.1016/0002-9343(64)90191-3. [DOI] [PubMed] [Google Scholar]
  8. FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Franklin E. C., Stanworth D. R. ANTIGENIC RELATIONSHIPS BETWEEN IMMUNE GLOBULINS AND CERTAIN RELATED PARAPROTEINS IN MAN. J Exp Med. 1961 Sep 30;114(4):521–533. doi: 10.1084/jem.114.4.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KATZ A. M., DREYER W. J., ANFINSEN C. B. Peptide separation by two-dimensional chromatography and electrophoresis. J Biol Chem. 1959 Nov;234:2897–2900. [PubMed] [Google Scholar]
  12. KORNGOLD L. Antigenic specificity of gamma2-myeloma globulins. J Natl Cancer Inst. 1963 Mar;30:553–567. [PubMed] [Google Scholar]
  13. KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
  14. MELTZER M., FRANKLIN E. C., FUNDENBERG H., FRANGIONE B. SINGLE PEPTIDE DIFFERENCES BETWEEN GAMMA-GLOBULINS OF DIFFERENT GENETIC (GM) TYPES. Proc Natl Acad Sci U S A. 1964 Jun;51:1007–1014. doi: 10.1073/pnas.51.6.1007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. NISONOFF A., WISSLER F. C., LIPMAN L. N. Properties of the major component of a peptic digest of rabbit antibody. Science. 1960 Dec 9;132(3441):1770–1771. doi: 10.1126/science.132.3441.1770. [DOI] [PubMed] [Google Scholar]
  16. OSSERMAN E. F., TAKATSUKI K. PLASMA CELL MYELOMA: GAMMA GLOBULIN SYNTHESIS AND STRUCTURE. A REVIEW OF BIOCHEMICAL AND CLINICAL DATA, WITH THE DESCRIPTION OF A NEWLY-RECOGNIZED AND RELATED SYNDROME, "H-GAMMA-2-CHAIN (FRANKLIN'S) DISEASE. Medicine (Baltimore) 1963 Nov;42:357–384. [PubMed] [Google Scholar]
  17. PAIN R. H. THE MOLECULAR WEIGHTS OF THE PEPTIDE CHAINS OF GAMMA-GLOBULIN. Biochem J. 1963 Aug;88:234–239. doi: 10.1042/bj0880234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PUTNAM F. W., EASLEY C. W., LYNN L. T. Site of cleavage of gamma-globulins by papain. Biochim Biophys Acta. 1962 Apr 9;58:279–290. doi: 10.1016/0006-3002(62)91010-7. [DOI] [PubMed] [Google Scholar]
  19. PUTNAM F. W. Plasma-cell myeloma and macroglobulinemia. I. Physicochemical, immunochemical and isotopic turnover studies of the abnormal serum and urinary proteins. N Engl J Med. 1959 Oct 29;261:902–908. doi: 10.1056/NEJM195910292611806. [DOI] [PubMed] [Google Scholar]
  20. SMITHIES O. An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959 Mar;71(3):585–587. doi: 10.1042/bj0710585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STEIN S., NACHMAN R. L., ENGLE R. L. INDIVIDUAL AND SUB-GROUP ANTIGENIC SPECIFICITY OF BENCE-JONES PROTEIN. Nature. 1963 Dec 21;200:1180–1181. doi: 10.1038/2001180a0. [DOI] [PubMed] [Google Scholar]
  22. TODD C. W. Allotypy in rabbit 19S protein. Biochem Biophys Res Commun. 1963 May 3;11:170–175. doi: 10.1016/0006-291x(63)90329-2. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES