Abstract
The fate of a heat-stable Escherichia coli agglutinogen within three types of rabbit phagocytic cells was examined. A system is described whereby quantitative ingestion of viable E. coli by suspensions of PMN leucocytes, BCG-induced alveolar macrophages, and oil-induced peritoneal macrophages took place in vitro. After various periods of intracellular residence aliquots were injected intraperitoneally into NCS mice and the resulting agglutinins assayed. The loss of immunogenicity within phagocytes was estimated by comparison with a dose-response titration prepared with bacteria alone. Under these conditions no increase in immunogenic mass occurred in vivo or in vitro when viable organisms were employed. PMN leucocytes and alveolar macrophages destroyed the majority of the immunogen within 2 hours of intracellular residence. In contrast, the immunogenicity of E. coli was maintained within peritoneal macrophages for periods up to 5 hours. The use of heat-killed bacilli or specific immune serum did not significantly influence the intracellular fate of the immunogen. Residual immunogenicity was associated with a particle having the same centrifugal properties as the intact organism and essentially none was released in a soluble form. Intracellular residence within phagocytic cells did not influence the resulting temporal sequence of antibody formation nor the proportions of mercaptoethanol-sensitive and resistant immune globulins.
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Selected References
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- COHN Z. A. Determinants of infection in the peritoneal cavity. I. Response to and fate of Staphylococcus aureus and Staphylococcus albus in the mouse. Yale J Biol Med. 1962 Aug;35:12–28. [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A., HIRSCH J. G. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:983–1004. doi: 10.1084/jem.112.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A. Influence of rabbit polymorphonuclear leucocytes and macrophages on the immunogenicity of Escherichia coli. Nature. 1962 Dec 15;196:1066–1068. doi: 10.1038/1961066a0. [DOI] [PubMed] [Google Scholar]
- COHN Z. A., MORSE S. I. Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J Exp Med. 1959 Sep 1;110:419–443. doi: 10.1084/jem.110.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A. The fate of bacteria within phagocytic cells. II. The modification of intracellular degradation. J Exp Med. 1963 Jan 1;117:43–53. doi: 10.1084/jem.117.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES. J Exp Med. 1963 Dec 1;118:991–1008. doi: 10.1084/jem.118.6.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. II. BIOCHEMICAL AND MORPHOLOGICAL RESPONSE TO PARTICLE INGESTION. J Exp Med. 1963 Dec 1;118:1009–1020. doi: 10.1084/jem.118.6.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
- HIRSCH J. G., COHN Z. A. Degranulation of polymorphonuclear leucocytes following phagocytosis of microorganisms. J Exp Med. 1960 Dec 1;112:1005–1014. doi: 10.1084/jem.112.6.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MYRVIK Q. N., LEAKE E. S., OSHIMA S. A study of macrophages and epitheloid-like cells from granulomatous (BCG-induced) lungs of rabbits. J Immunol. 1962 Nov;89:745–751. [PubMed] [Google Scholar]
- SORKIN E., BOYDEN S. V. Studies on the fate of antigens in vitro. I. The effect of specific antibody on the fate of I 131 trace labeled human serum albumin in vitro in the presence of guinea pig monocytes. J Immunol. 1959 Apr;82(4):332–339. [PubMed] [Google Scholar]
- WALSH T. E., SMITH C. A. The influence of polymorphonuclear leukocytes and macrophages on antibody production. J Immunol. 1951 Mar;66(3):303–310. [PubMed] [Google Scholar]