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. 1964 Oct 31;120(5):795–804. doi: 10.1084/jem.120.5.795

RUNT DISEASE INDUCED IN NEONATAL MICE BY STERILE BACTERIAL VACCINES

Richard D Ekstedt 1, Edwin T Nishimura 1
PMCID: PMC2137872  PMID: 14247720

Abstract

A form of runt disease has been produced in neonatal CF-1 and ICR mice by the repeated injection of 109 washed, autoclaved, saline-suspended staphylococci or streptococci. The most severely affected animals showed a marked decrease in lymphoid tissues and resembled grossly and microscopically animals suffering from the classical runt or wasting disease described by others. The timing of the initial antigenic stimulation was of importance, and animals started on their course of injections at an age of 48 hours or older showed no effect. There was a considerable variation in the severity of the disease within litters and from one litter to another. This variation could not be ascribed to a difference in susceptibility between sexes, since both male and female mice were observed to runt under appropriate conditions. Germ-free ICR mice were much more resistant to the runting phenomenon than conventional animals of the same strain, but could be induced to runt by injection of the staphylococcal vaccine suspended in homologous antiserum. The relationship of the runting phenomenon described here and classical runt disease or runting by adrenal hormones is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARNES D. W., LOUTIT J. F., MICKLEM H. S. "Secondary disease" of radiation chimeras: a syndrome due to lymphoid aplasia. Ann N Y Acad Sci. 1962 Oct 24;99:374–385. doi: 10.1111/j.1749-6632.1962.tb45321.x. [DOI] [PubMed] [Google Scholar]
  2. BILLINGHAM R. E., BRENT L. A simple method for inducing tolerance of skin homografts in mice. Transplant Bull. 1957 Apr;4(2):67–71. [PubMed] [Google Scholar]
  3. BLAESE R. M., MARTINEZ C., GOOD R. A. IMMUNOLOGIC INCOMPETENCE OF IMMUNOLOGICALLY RUNTED ANIMALS. J Exp Med. 1964 Feb 1;119:211–224. doi: 10.1084/jem.119.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COHEN J. O., NEWTON W. L., CHERRY W. B., UPDYKE E. L. NORMALLY OCCURRING STAPHYLOCOCCAL ANTIBODIES IN GERMFREE MICE. J Immunol. 1963 Mar;90:358–367. [PubMed] [Google Scholar]
  5. EKSTEDT R. D. STUDIES ON IMMUNITY TO STAPHYLOCOCCAL INFECTION IN MICE. III. A PROTEIN ANTIGEN THAT INDUCES PROTECTION AGAINST CHALLENGE WITH SALINE-SUSPENDED ORGANISMS. J Infect Dis. 1963 Sep-Oct;113:105–109. doi: 10.1093/infdis/113.2.105. [DOI] [PubMed] [Google Scholar]
  6. HOTCHIN J., WEIGAND H. Studies of lymphocytic choriomeningitis in mice. I. The relationship between age at inoculation and outcome of infection. J Immunol. 1961 Apr;86:392–400. [PubMed] [Google Scholar]
  7. HUNT G. A., MOSES A. J. Acute infection of mice with Smith strain of Staphylococcus aureus. Science. 1958 Dec 19;128(3338):1574–1575. doi: 10.1126/science.128.3338.1574. [DOI] [PubMed] [Google Scholar]
  8. ROWE W. P., CAPPS W. I. A new mouse virus causing necrosis of the thymus in newborn mice. J Exp Med. 1961 May 1;113:831–844. doi: 10.1084/jem.113.5.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SCHLESINGER M., MARK R. WASTING DISEASE INDUCED IN YOUNG MICE BY ADMINISTRATION OF CORTISOL ACETATE. Science. 1964 Feb 28;143(3609):965–966. doi: 10.1126/science.143.3609.965. [DOI] [PubMed] [Google Scholar]
  10. STANLEY N. F. Reovirus--a ubiquitous orphan. Med J Aust. 1961 Nov 18;48(2):815–818. [PubMed] [Google Scholar]

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