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. 1964 Feb 29;119(3):443–452. doi: 10.1084/jem.119.3.443

STUDIES ON HUMAN ANTIBODIES

I. STARCH GEL ELECTROPHORESIS OF THE DISSOCIATED POLYPEPTIDE CHAINS

G M Edelman 1, Elvin A Kabat 1
PMCID: PMC2137884  PMID: 14129715

Abstract

Specific precipitates and purified human antibodies were reduced and alkylated and subjected to starch gel electrophoresis in 8 M urea to dissociate and separate the L and H polypeptide chains. Dissociated antibodies to dextran, levan, teichoic acid, blood group A substance, and tetanus toxoid showed sharp bands corresponding to L polypeptide chains. The patterns differed for antibodies of different specificities. Some differences were also seen among antibodies of the same specificity from different individuals. Purified antidextran antibodies showed particularly simple patterns resembling those of purified human γ myeloma proteins. In some cases only one L chain band was present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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