Abstract
1. Immunochemical cross-reactivity, but not identity, has been demonstrated between bovine parathyroid hormone and an antigen in biologically active extracts of human parathyroid tissue by quantitative C' fixation and C' fixation inhibition. 2. An antigen that fixes C' with rabbit antibody to bovine parathyroid hormone has been found in urea extracts of six human non-parathyroid neoplasms associated with a hypercalcemic syndrome mimicking primary hyperparathyroidism. Comparable extracts of control tissues and other tumors were serologically negative. 3. It is concluded that the tumor antigen is parathyroid hormone or a very closely related protein, and that its production by these neoplasms was the cause of the hypercalcemic syndrome in these six patients.
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Selected References
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- AURBACH G. D. Isolation of parathyroid hormone after extraction with phenol. J Biol Chem. 1959 Dec;234:3179–3181. [PubMed] [Google Scholar]
- Berson S. A., Yalow R. S., Aurbach G. D., Potts J. T. IMMUNOASSAY OF BOVINE AND HUMAN PARATHYROID HORMONE. Proc Natl Acad Sci U S A. 1963 May;49(5):613–617. doi: 10.1073/pnas.49.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HEALY J. W., STOLLAR D., SIMON M. I., LEVINE L. CHARACTERIZATION OF A PHOSPHODIESTERASE FROM LAMB BRAIN. Arch Biochem Biophys. 1963 Dec;103:461–468. doi: 10.1016/0003-9861(63)90439-9. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- ODELL W. D., BATES R. W., RIVLIN R. S., LIPSETT M. B., HERTZ R. Increased thyroid function without clinical hyperthyroidism in patients with choriocarcinoma. J Clin Endocrinol Metab. 1963 Jul;23:658–664. doi: 10.1210/jcem-23-7-658. [DOI] [PubMed] [Google Scholar]
- RASMUSSEN H., CRAIG L. C. Purification of bovine parathyroid hormone by gel filtration. Biochim Biophys Acta. 1962 Jan 29;56:332–338. doi: 10.1016/0006-3002(62)90571-1. [DOI] [PubMed] [Google Scholar]
- REICHLIN M., HAY M., LEVINE L. IMMUNOCHEMICAL STUDIES OF HEMOGLOBIN AND MYOGLOBIN AND THEIR GLOBIN MOIETIES. Biochemistry. 1963 Sep-Oct;2:971–979. doi: 10.1021/bi00905a013. [DOI] [PubMed] [Google Scholar]
- SAMUELSSON S. M., WERNER I. Hepatic carcinoma simulating hyperparathyroidism. Acta Med Scand. 1963 May;173:539–547. doi: 10.1111/j.0954-6820.1963.tb17438.x. [DOI] [PubMed] [Google Scholar]
- TASHJIAN A. H., Jr, LEVINE L., MUNSON P. L. IMMUNOASSAY OF PARATHYROID HORMONE BY QUANTITATIVE COMPLEMENT FIXATION. Endocrinology. 1964 Feb;74:244–254. doi: 10.1210/endo-74-2-244. [DOI] [PubMed] [Google Scholar]
- TASHJIAN A. H., Jr, LEVINE L., MUNSON P. L. Production of an antibody to bovine parathyroid hormone. Biochem Biophys Res Commun. 1962 Jul 19;8:259–265. doi: 10.1016/0006-291x(62)90274-7. [DOI] [PubMed] [Google Scholar]
- TASHJIAN A. H., Jr, MUNSON P. L. Chemical nature and properties of parathyroid hormones. J Chronic Dis. 1963 Apr;16:269–281. doi: 10.1016/0021-9681(63)90077-8. [DOI] [PubMed] [Google Scholar]
- VAN VUNAKIS H., LEHRER H. I., ALLISON W. S., LEVINE L. Immunochemical studies on the components of the pepsinogen system. J Gen Physiol. 1963 Jan;46:589–604. doi: 10.1085/jgp.46.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VAN VUNAKIS H., LEVINE L. Structural studies on pepsinogen and pepsin: an immunological approach. Ann N Y Acad Sci. 1963 May 8;103:735–743. doi: 10.1111/j.1749-6632.1963.tb53730.x. [DOI] [PubMed] [Google Scholar]
- WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]