Abstract
Experiments carried out with several well defined antigenic systems (hapten conjugates of poly-L-lysine and guinea pig serum albumin) in guinea pigs demonstrated that: 1. Arthus reactions also manifest carrier specificity, although to a smaller extent than do delayed hypersensitivity reactions. 2. Desensitization by injection of minute doses of antigen results in moderate specific desensitization of delayed hypersensitivity without desensitization of Arthus reactivity to the same antigenic determinant. 3. Insoluble antigen-antibody complexes prepared from high affinity guinea pig antibodies can elicit specific delayed skin reactions in sensitized guinea pigs. 4. Homologous conjugates of structurally similar haptens show considerably less cross-reactivity in delayed reactions than in immediate hypersensitivity reactions to the same antigenic determinant. These experimental results are interpreted as indicating that delayed hypersensitivity reactions in the guinea pig are mediated by "antibodies" of comparatively high binding affinities. High binding affinities are achieved for these antibodies more likely by closer structural adaptation between antigen and antibody than by a larger area of specific contact.
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Selected References
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- BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUCHANAN-DAVIDSON D. J., DELLERT E. E., KORNGUTH S. E., STAHMANN M. A. Immunochemistry of synthetic polypeptides and polypeptidyl proteins. II. Quantitative studies on the modified protiens. J Immunol. 1959 Nov;83:543–551. [PubMed] [Google Scholar]
- CEBRA J. J. Studies on the combining sites of the protein antigen silk fibroin. III. Inhibition of the silk fibroin-antifibroin system by peptides derived from the antigen. J Immunol. 1961 Feb;86:205–214. [PubMed] [Google Scholar]
- EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
- EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
- GELL P. G., SILVERSTEIN A. M. Delayed hypersensitivity to hapten-protein conjugates. I. The effect of carrier protein and site of attachment to hapten. J Exp Med. 1962 May 1;115:1037–1051. doi: 10.1084/jem.115.5.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KABAT E. A. Heterogeneity in extent of the combining regions of human antidextran. J Immunol. 1956 Dec;77(6):377–385. [PubMed] [Google Scholar]
- KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAWRENCE H. S., PAPPENHEIMER A. M., Jr Transfer of delayed hypersensitivity to diphtheria toxin in man. J Exp Med. 1956 Sep 1;104(3):321–335. doi: 10.1084/jem.104.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAWRENCE H. S. The delayed type of allergic inflammatory response. Am J Med. 1956 Mar;20(3):428–447. doi: 10.1016/0002-9343(56)90128-0. [DOI] [PubMed] [Google Scholar]
- LEVINE B. B. N(ALPHA-D-PENICILLOYL) AMINES AS UNIVALENT HAPTEN INHIBITORS OF ANTIBODYDEPENDENT ALLERGIC REACTIONS TO PENICILLIN. J Med Pharm Chem. 1962 Sep;91:1025–1034. doi: 10.1021/jm01240a016. [DOI] [PubMed] [Google Scholar]
- LEVINE B. B., OJEDA A., BENACERRAF B. BASIS FOR THE ANTIGENICITY OF HAPTEN-POLY-L-LYSINE CONJUGATES IN RANDOM-BRED GUINEA PIGS. Nature. 1963 Nov 9;200:544–546. doi: 10.1038/200544a0. [DOI] [PubMed] [Google Scholar]
- LEVINE B. B., PRICE V. H. STUDIES ON THE IMMUNOLOGICAL MECHANISMS OF PENICILLIN ALLERGY. II. ANTIGENIC SPECIFICITIES OF ALLERGIC WHEAL-AND-FLARE SKIN RESPONSES IN PATIENTS WITH HISTORIES OF PENICILLIN ALLERGY. Immunology. 1964 Sep;7:542–556. [PMC free article] [PubMed] [Google Scholar]
- LEVINE B. B. STUDIES ON THE IMMUNOLOGICAL MECHANISMS OF PENICILLIN ALLERGY. I. ANTIGENIC SPECIFICITIES OF GUINEA-PIG SKIN SENSITIZING RABBIT ANTI-BENZYLPENICILLIN ANTIBODIES. Immunology. 1964 Sep;7:527–541. [PMC free article] [PubMed] [Google Scholar]
- LEVINE B. B. Studies on the dimensions of the rabbit antibenzylpenicilloyl antibody-combing sites. J Exp Med. 1963 Jan 1;117:161–183. doi: 10.1084/jem.117.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. The specificity of allergic reactions. I. Delayed versus Arthus hypersensitivity. J Exp Med. 1960 Apr 1;111:465–483. doi: 10.1084/jem.111.4.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SILVERSTEIN A. M., GELL P. G. Delayed hypersensitivity to hapten-protein conjugates. II. Anti-hapten specificity and the heterogeneity of the delayed response. J Exp Med. 1962 May 1;115:1053–1064. doi: 10.1084/jem.115.5.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
- UHR J. W., PAPPENHEIMER A. M., Jr Delayed hypersensitivity. III. Specific desensitization of guinea pigs sensitized to protein antigens. J Exp Med. 1958 Dec 1;108(6):891–904. doi: 10.1084/jem.108.6.891. [DOI] [PMC free article] [PubMed] [Google Scholar]