Abstract
The pathogenesis of Mycoplasma pneumoniae infection was studied in the Syrian hamster with qualitative and quantitative culture methods and special histopathologic techniques. The animals were readily infected with the mycoplasma, which multiplied throughout the respiratory tract. Sensitivity of this experimental host to infection was indicated by the 50 per cent infective dose, which was 10 colony-forming units of the organism. Inoculation consistently resulted in the production of peribronchial pneumonitis which was induced by the mycoplasma. The organisms were visualized in a superficial location in the mucosa of involved bronchi, by means of indirect fluorescent antibody staining and by a modification of the Brown and Brenn technique. The data indicate applicability of the hamster to the study of problems concerned with M. pneumoniae disease which are impractical or impossible to resolve in the human host.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLYDE W. A., Jr, DENNY F. W., DINGLE J. H. Fluorescent-stainable antibodies to the Eaton agent in human primary atypical pneumonia transmission studies. J Clin Invest. 1961 Sep;40:1638–1647. doi: 10.1172/JCI104386. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLYDE W. A., Jr, DENNY F. W., Jr THE ETIOLOGY AND THERAPY OF ATYPICAL PNEUMONIA. Med Clin North Am. 1963 Sep;47:1201–1218. doi: 10.1016/s0025-7125(16)33528-3. [DOI] [PubMed] [Google Scholar]
- CLYDE W. A., Jr Demonstration of Eaton's agent in tissue culture. Proc Soc Exp Biol Med. 1961 Aug-Sep;107:715–718. doi: 10.3181/00379727-107-26732. [DOI] [PubMed] [Google Scholar]
- COUCH R. B., CATE T. R., CHANOCK R. M. INFECTION WITH ARTIFICIALLY PROPAGATED EATON AGENT (MYCOPLASMA PNEUMONIAE). IMPLICATIONS FOR DEVELOPMENT OF ATTENUATED VACCINE FOR COLD AGGLUTININ-POSITIVE PNEUMONIA. JAMA. 1964 Feb 8;187:442–447. [PubMed] [Google Scholar]
- Eaton M. D., Meiklejohn G., van Herick W. STUDIES ON THE ETIOLOGY OF PRIMARY ATYPICAL PNEUMONIA : A FILTERABLE AGENT TRANSMISSIBLE TO COTTON RATS, HAMSTERS, AND CHICK EMBRYOS. J Exp Med. 1944 Jun 1;79(6):649–668. doi: 10.1084/jem.79.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOODBURN G. M., MARMION B. P. A study of the properties of Eaton's primary atypical pneumonia organism. J Gen Microbiol. 1962 Oct;29:271–290. doi: 10.1099/00221287-29-2-271. [DOI] [PubMed] [Google Scholar]
- GRAYSTON J. T., ALEXANDER E. R., KENNY G. E., CLARKE E. R., FREMONT J. C., MACCOLL W. A. MYCOPLASMA PNEUMONIAE INFECTIONS. CLINICAL AND EPIDEMIOLOGIC STUDIES. JAMA. 1965 Feb 1;191:369–374. doi: 10.1001/jama.1965.03080050015004. [DOI] [PubMed] [Google Scholar]
- MARMION B. P., GOODBURN G. M. Effect of an organic gold salt on Eaton's primary atypical pneumonia agent and other observations. Nature. 1961 Jan 21;189:247–248. doi: 10.1038/189247a0. [DOI] [PubMed] [Google Scholar]
- Nelson J. B. STUDIES ON AN UNCOMPLICATED CORYZA OF THE DOMESTIC FOWL : VI. COCCOBACILLIFORM BODIES IN BIRDS INFECTED WITH THE CORYZA OF SLOW ONSET. J Exp Med. 1936 Mar 31;63(4):515–522. doi: 10.1084/jem.63.4.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabin A. B. ISOLATION OF A FILTRABLE, TRANSMISSIBLE AGENT WITH "NEUROLYTI" PROPERTIES FROM TOXOPLASMA-INFECTED TISSUES. Science. 1938 Aug 26;88(2278):189–191. doi: 10.1126/science.88.2278.189. [DOI] [PubMed] [Google Scholar]