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. 1965 Aug 1;122(2):277–298. doi: 10.1084/jem.122.2.277

ISOLATION OF β1F-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT

Ulf R Nilsson 1, Hans J Müller-Eberhard 1
PMCID: PMC2138056  PMID: 14316946

Abstract

At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors is the earlier described β1C-globulin. The second, a heretofore unrecorded serum protein, β1F-globulin. The third factor has not yet been defined as a discrete serum protein entity. Kinetic experiments indicated that β1C reacted prior to β1F, which in turn seemed to precede the third factor in the reaction sequence. Therefore, the 3 components were tentatively designated the third (C'3), the fifth (C'5), and the sixth (C'6) components of complement, respectively. A procedure was developed allowing the isolation of highly purified β1C-(C'3) and β1F-globulin (C'5) and of partially purified C'6. With respect to its function in immune hemolysis, β1F-globulin or C'5 was found to be closely dependent on the simultaneous presence of C'6. The hypothesis that C'5 and C'6 form a functional unit was supported by the finding that both components interact with each other in solution resulting in the formation of a complex. A similar complex was also found in fresh human serum.

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Selected References

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