Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 Oct;169(10):4451–4456. doi: 10.1128/jb.169.10.4451-4456.1987

Protein synthesis during encystment of Azotobacter vinelandii.

C J Su 1, A da Cunha 1, C M Wernette 1, R N Reusch 1, H L Sadoff 1
PMCID: PMC213807  PMID: 3654578

Abstract

Proteins synthesized during the encystment of Azotobacter vinelandii were radiolabeled with [35S]methionine and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Pulse labeling was used to demonstrate that early encystment-specific proteins were beginning to be synthesized at 2 h and reached peak levels about 12 h after initiation of encystment. One such protein was identified as a beta-ketoacyl acyl-carrier protein synthase. The concentration of early proteins began to decrease at 16 h, when intermediate proteins specific to the differentiation process began to be synthesized. The cessation of synthesis of intermediate proteins began at 20 h postinitiation, and the labeling pattern of proteins then remained constant throughout the remaining 4 days of encystment.

Full text

PDF
4451

Images in this article

4452Image
on p.4452
4454Image
on p.4454

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F., Nikaido K. Two-dimensional gel electrophoresis of membrane proteins. Biochemistry. 1976 Feb 10;15(3):616–623. doi: 10.1021/bi00648a026. [DOI] [PubMed] [Google Scholar]
  2. Hitchins V. M., Sadoff H. L. Sequential metabolic events during encystment of Azobacter vinelandii. J Bacteriol. 1973 Mar;113(3):1273–1279. doi: 10.1128/jb.113.3.1273-1279.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Inouye M., Yee M. L. Homogeneity of envelope proteins of Escherichia coli separated by gel electrophoresis in sodium dodecyl sulfate. J Bacteriol. 1973 Jan;113(1):304–312. doi: 10.1128/jb.113.1.304-312.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kass L. R., Brock D. J., Bloch K. Beta-hydroxydecanoyl thioester dehydrase. I. Purification and properties. J Biol Chem. 1967 Oct 10;242(19):4418–4431. [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lin L. P., Sadoff H. L. Encystment and polymer production by Azotobacter vinelandii in the presence of beta-hydroxybutyrate. J Bacteriol. 1968 Jun;95(6):2336–2343. doi: 10.1128/jb.95.6.2336-2343.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Linn T., Losick R. The program of protein synthesis during sporulation in Bacillus subtilis. Cell. 1976 May;8(1):103–114. doi: 10.1016/0092-8674(76)90191-4. [DOI] [PubMed] [Google Scholar]
  8. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  9. Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reusch R. N., Sadoff H. L. 5-n-Alkylresorcinols from encysting Azotobacter vinelandii: isolation and characterization. J Bacteriol. 1979 Aug;139(2):448–453. doi: 10.1128/jb.139.2.448-453.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reusch R. N., Sadoff H. L. Novel lipid components of the Azotobacter vinelandii cyst membrane. Nature. 1983 Mar 17;302(5905):268–270. doi: 10.1038/302268a0. [DOI] [PubMed] [Google Scholar]
  12. Ruppen M. E., Garner G., Sadoff H. L. Protein turnover in Azotobacter vinelandii during encystment and germination. J Bacteriol. 1983 Dec;156(3):1243–1248. doi: 10.1128/jb.156.3.1243-1248.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sadoff H. L., Berke E., Loperfido B. Physiological studies of encystment in Azotobacter vinelandii. J Bacteriol. 1971 Jan;105(1):185–189. doi: 10.1128/jb.105.1.185-189.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sadoff H. L., Shimel B., Ellis S. Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes. J Bacteriol. 1979 Jun;138(3):871–877. doi: 10.1128/jb.138.3.871-877.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Su C. J., Reusch R. N., Sadoff H. L. Isolation and characterization of several unique lipids from Azotobacter vinelandii cysts. J Bacteriol. 1981 Jul;147(1):80–90. doi: 10.1128/jb.147.1.80-90.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Su C. J., Reusch R., Sadoff H. L. Fatty acids in phospholipids of cells, cysts, and germinating cysts of Azotobacter vinelandii. J Bacteriol. 1979 Mar;137(3):1434–1436. doi: 10.1128/jb.137.3.1434-1436.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Su C. J., Sadoff H. L. Unique lipids in Azotobacter vinelandii cysts: synthesis, distribution, and fate during germination. J Bacteriol. 1981 Jul;147(1):91–96. doi: 10.1128/jb.147.1.91-96.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Toomey R. E., Wakil S. J. Studies on the mechanism of fatty acid synthesis. XVI. Preparation and general properties of acyl-malonyl acyl carrier protein-condensing enzyme from Escherichia coli. J Biol Chem. 1966 Mar 10;241(5):1159–1165. [PubMed] [Google Scholar]
  19. Youngman P., Zuber P., Perkins J. B., Sandman K., Igo M., Losick R. New ways to study developmental genes in spore-forming bacteria. Science. 1985 Apr 19;228(4697):285–291. doi: 10.1126/science.228.4697.285. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES