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. 1965 Sep 1;122(3):483–504. doi: 10.1084/jem.122.3.483

HUMORAL AND CELLULAR ASPECTS OF THE IMMUNE RESPONSE TO THE SOMATIC ANTIGEN OF SALMONELLA ENTERITIDIS

Maurice Landy 1, Ronald P Sanderson 1, Anne L Jackson 1
PMCID: PMC2138075  PMID: 5839282

Abstract

A study was made of the cellular and humoral aspects of the immune response of the rabbit to the somatic polysaccharide of Salmonella enteritidis. The response to a single intravenous injection was characterized by the appearance of elevated titers of bactericidal antibody between 2 and 3 days later. The maximum titer was dose-dependent and occurred between 5 and 7 days, thereafter declining rapidly during the first month. The significant stabilized levels which then persisted for at least 1 year were also dose-dependent. Most of the antibody produced (>99 per cent) was associated with the macroglobulin fraction of serum. Plaque-forming cells (PFC) elaborating antibody specific for this somatic antigen were detected and enumerated by the technique of localized hemolysis in gel employing polysaccharide-coated sheep erythrocytes. Significant numbers of PFC were encountered in the spleen as early as 14 to 18 hours after a single intravenous injection of antigen; after 36 hours the number of PFC rose rapidly and culminated in a maximum population at 5 days, followed by a rapid decline and plateau similar to that for circulating antibody. The spleen was the principal organ involved in the systemic response, but other lymphoid tissues including bone marrow, peripheral blood leucocytes, and thymus contributed significantly. After an interval of 3 months the effect on humoral antibody titers of a second injection of antigen was dependent on the amount of polysaccharide administered; markedly greater titers were now obtained with 0.02 to 0.002 µg, whereas 0.2 to 20 µg resulted in a duplication of the initial humoral response. The cellular response to a second dose of 5 µg was accelerated; larger numbers of PFC appeared more rapidly, attained a maximum population by day 3, and exceeded the primary response by a factor of two. This acceleration in the attainment of maximum numbers of PFC and the increased bactericidal antibody titers following a second injection of limiting amounts of antigen suggest that these somatic polysaccharides may in fact evoke a "secondary" type of response in the rabbit.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER F. L. On hemolysis mediated by non-erythrocytic antigens, their homologous antibodies and complement. Proc Soc Exp Biol Med. 1950 Jul;74(3):561–565. doi: 10.3181/00379727-74-17972. [DOI] [PubMed] [Google Scholar]
  2. BAUER D. C., MATHIES M. J., STAVITSKY A. B. Sequences of synthesis of gamma-1 macroglobulin and gamma-2 globulin antibodies during primary and secondary responses to proteins, salmonella antigens, and phage. J Exp Med. 1963 Jun 1;117:889–907. doi: 10.1084/jem.117.6.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FRISCH A. W., DAVIES G. H. Inhibition of hemagglutinin formation by thioguanine: dose-time relationships. Proc Soc Exp Biol Med. 1962 Jul;110:444–447. doi: 10.3181/00379727-110-27544. [DOI] [PubMed] [Google Scholar]
  4. HULLIGER L., SORKIN E. Synthesis of antibodies by blood leucocytes of the rabbit. Nature. 1963 Apr 20;198:299–299. doi: 10.1038/198299a0. [DOI] [PubMed] [Google Scholar]
  5. INGRAHAM J. S., BUSSARD A. APPLICATION OF A LOCALIZED HEMOLYSIN REACTION FOR SPECIFIC DETECTION OF INDIVIDUAL ANTIBODY-FORMING CELLS. J Exp Med. 1964 Apr 1;119:667–684. doi: 10.1084/jem.119.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOHNSON A. G., GAINES S., LANDY M. Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide. J Exp Med. 1956 Feb 1;103(2):225–246. doi: 10.1084/jem.103.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LANDY M., JOHNSON A. G., WEBSTER M. E., SAGIN J. F. Studies on the O antigen of Salmonella typhosa. II. Immunological properties of the purified antigen. J Immunol. 1955 Jun;74(6):466–478. [PubMed] [Google Scholar]
  8. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  9. LANDY M., SANDERSON R. P., BERNSTEIN M. T., LERNER E. M., 2nd INVOLVEMENT OF THYMUS IN IMMUNE RESPONSE OF RABBITS TO SOMATIC POLYSACCHARIDES OF GRAM-NEGATIVE BACTERIA. Science. 1965 Mar 26;147(3665):1591–1592. doi: 10.1126/science.147.3665.1591. [DOI] [PubMed] [Google Scholar]
  10. LANDY M., TRAPANI R. J., CLARK W. R. Studies on the O antigen of Salmonella typhosa. III. Activity of the isolated antigen in the hemagglutination procedure. Am J Hyg. 1955 Jul;62(1):54–65. doi: 10.1093/oxfordjournals.aje.a119766. [DOI] [PubMed] [Google Scholar]
  11. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  12. MERRITT K., JOHNSON A. G. STUDIES ON THE ADJUVANT ACTION OF BACTERIAL ENDOTOXINS ON ANTIBODY FORMATION. VI. ENHANCEMENT OF ANTIBODY FORMATION BY NUCLEIC ACIDS. J Immunol. 1965 Mar;94:416–422. [PubMed] [Google Scholar]
  13. MULHOLLAND J. H., WOLFF S. M., JACKSON A. L., LANDY M. QUANTITATIVE STUDIES OF FEBRILE TOLERANCE AND LEVELS OF SPECIFIC ANTIBODY EVOKED BY BACTERIAL ENDOTOXIN. J Clin Invest. 1965 Jun;44:920–928. doi: 10.1172/JCI105209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PIKE R. M., SCHULZE M. L. PRODUCTION OF 7S AND 19S ANTIBODIES TO THE SOMATIC ANTIGENS OF SALMONELLA TYPHOSA IN RABBITS. Proc Soc Exp Biol Med. 1964 Mar;115:829–833. doi: 10.3181/00379727-115-29050. [DOI] [PubMed] [Google Scholar]
  15. RIBI E., HASKINS W. T., LANDY M., MILNER K. C. Preparation and host-reactive properties of endotoxin with low content of nitrogen and lipid. J Exp Med. 1961 Nov 1;114:647–663. doi: 10.1084/jem.114.5.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. STAUB A. M. Role des anticorps antipolyosidiques dans l'agglutination des bacilles typhiques. Ann Inst Pasteur (Paris) 1954 May;86(5):618–635. [PubMed] [Google Scholar]
  17. STELOS P., TALMAGE D. W. The separation by starch electrophoresis of two antibodies in sheep red cells differing in hemolytic efficiency. J Infect Dis. 1957 Mar-Apr;100(2):126–135. doi: 10.1093/infdis/100.2.126. [DOI] [PubMed] [Google Scholar]
  18. STONER R. D., BOND V. P. ANTIBODY FORMATION BY TRANSPLANTED BONE MARROW, SPLEEN, LYMPH NODES AND THYMUS CELLS IN IRRADIATED RECIPIENTS. J Immunol. 1963 Aug;91:185–196. [PubMed] [Google Scholar]
  19. TALIAFERRO W. H., TALMAGE D. W. Antibodies in the rabbit with different rates of metabolic decay. J Infect Dis. 1956 Jul-Aug;99(1):21–33. doi: 10.1093/infdis/99.1.21. [DOI] [PubMed] [Google Scholar]
  20. TALMAGE D. W., DIXON F. J., BUKANTZ S. C., DAMMIN G. J. Antigen elimination from the blood as an early manifestation of the immune response. J Immunol. 1951 Oct;67(4):243–255. [PubMed] [Google Scholar]
  21. WEIDANZ W. P., JACKSON A. L., LANDY M. SOME ASPECTS OF THE ANTIBODY RESPONSE OF RABBITS TO IMMUNIZATION WITH ENTEROBACTERIAL SOMATIC ANTIGENS. Proc Soc Exp Biol Med. 1964 Jul;116:832–837. doi: 10.3181/00379727-116-29386. [DOI] [PubMed] [Google Scholar]
  22. WEIDANZ W. P., LANDY M. A SIMPLIFIED METHOD FOR BACTERICIDAL ASSAY OF NATURAL ANTIBODIES AGAINST GRAM-NEGATIVE BACTERIA. Proc Soc Exp Biol Med. 1963 Aug-Sep;113:861–867. doi: 10.3181/00379727-113-28513. [DOI] [PubMed] [Google Scholar]

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