Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1965 Oct 31;122(5):943–954. doi: 10.1084/jem.122.5.943

STUDIES OF ADENOVIRUS SV40 HYBRID VIRUSES

I. ASSAY SYSTEM AND FURTHER EVIDENCE FOR HYBRIDIZATION

Wallace P Rowe 1, Stephen G Baum 1, Wendell E Pugh 1, M David Hoggan 1
PMCID: PMC2138118  PMID: 4285252

Abstract

The titers of adenovirus and SV40 genetic carriers in hybrid preparations (E46+) can be quantitated by determining the percentage of cells showing neoantigens stainable with fluorescent antibody (FA) at 21 to 24 hours; both titers can be obtained with a single coverslip. The adenovirus and SV40 antigen-inducing titers so obtained are of the same order of magnitude in stock preparations of E46+ grown in either African green monkey kidney (AGMK) or human embryonic kidney tissue culture (HEK), and are generally within one log of the infectivity titer. Quantitative studies of 50°C heat inactivation, ultracentrifugation, and equilibrium density gradient centrifugation of E46+ gave no indication that SV40 neoantigen induction could be dissociated from adenovirus, whereas adenovirus and SV40 virus grown as a mixed infection were readily dissociated by these procedures. Pretreatment of HEK cells with a medium containing 5-fluorouracil desoxyriboside (FUDR) did not affect induction of either adenovirus or SV40 neoantigen or development of cytopathic effects after infection with E46+, but did prevent formation of Ad. 7 viral antigen.

Full Text

The Full Text of this article is available as a PDF (617.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  2. BLACK P. H., ROWE W. P., COOPER H. L. AN ANALYSIS OF SV 40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO. II. STUDIES OF THREE CLONES DERIVED FROM A CONTINUOUS LINE OF TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Nov;50:847–854. doi: 10.1073/pnas.50.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLACK P. H., ROWE W. P., TURNER H. C., HUEBNER R. J. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Dec;50:1148–1156. doi: 10.1073/pnas.50.6.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GILDEN R. V., CARP R. I., TAGUCHI F., DEFEND V. THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN. Proc Natl Acad Sci U S A. 1965 Mar;53:684–692. doi: 10.1073/pnas.53.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUEBNER R. J., ROWE W. P., TURNER H. C., LANE W. T. SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS. Proc Natl Acad Sci U S A. 1963 Aug;50:379–389. doi: 10.1073/pnas.50.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MEYER H. M., Jr, HOPPS H. E., ROGERS N. G., BROOKS B. E., BERNHEIM B. C., JONES W. P., NISALAK A., DOUGLAS R. D. Studies on simian virus 40. J Immunol. 1962 Jun;88:796–806. [PubMed] [Google Scholar]
  8. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RAPP F., BUTEL J. S., MELNICK J. L. VIRUS-INDUCED INTRANUCLEAR ANTIGEN IN CELLS TRANSFORMED BY PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1131–1135. doi: 10.3181/00379727-116-29472. [DOI] [PubMed] [Google Scholar]
  10. RAPP F., MELNICK J. L., BUTEL J. S., KITAHARA T. THE INCORPORATION OF SV40 MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGEN. Proc Natl Acad Sci U S A. 1964 Dec;52:1348–1352. doi: 10.1073/pnas.52.6.1348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RAPP F., MELNICK J. L., KITAHARA T. TUMOR AND VIRUS ANTIGENS OF SIMIAN VIRUS 40: DIFFERENTIAL INHIBITION OF SYNTHESIS BY CYTOSINE ARABINOSIDE. Science. 1965 Feb 5;147(3658):625–627. doi: 10.1126/science.147.3658.625. [DOI] [PubMed] [Google Scholar]
  12. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES