Abstract
Uremic colitis of varying severity occurred in the majority of conventionalized rats dying after removal of both kidneys, but was not found in uremic conventionalized and germfree rats sacrificed preterminally, or in germfree and limited-flora rats dying from uremia, or in any of the controls. The lesions were restricted to the cecum and their incidence and severity paralleled a shorter duration of survival. Cardiovascular damage including focal myocardial necrosis and calcification and patchy aortic and coronary calcification were observed in uremic rats regardless of their microbial status. These lesions had a higher incidence, developed more rapidly, and were more severe in the germfree and limited-flora rats than in the conventionalized animals. The presence or severity of the lesions, however, did not correlate with survival time of rats dying from uremia or with total plasma calcium and inorganic phosphorus levels of individual animals. Generalized necrotizing arteritis was not observed. Wound healing was poor in all uremic rats regardless of microbial status. Focal infection was noted in a few conventionalized rats dying from uremia, did not correlate with survival time, and was absent in all other groups. Comparison of biochemical findings between uremic germfree and conventionalized rats show a higher blood urea nitrogen and elevated plasma indoxyl sulfate in the presence of a microbial flora and a greater amount of plasma inorganic phosphorus in its absence. Uremia resulted in a decrease in hematocrit and increase in plasma and muscle potassium that were similar for germfree and conventionalized rats. Plasma and muscle sodium, total plasma calcium, glucose, and total protein were essentially unchanged by microbial status or uremia. Because the foregoing differences in the metabolic and histopathologic changes of uremia are linked to the known difference in microbial status of the fasting bilaterally-nephrectomized rats that were studied, they are a tangible indication of ways by which the indigenous microbial flora and its composition may affect the course of acute uremia.
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Selected References
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- BAUER H., HOROWITZ R. E., LEVENSON S. M., POPPER H. The response of the lymphatic tissue to the microbial flora. Studies on germfree mice. Am J Pathol. 1963 Apr;42:471–483. [PMC free article] [PubMed] [Google Scholar]
- BEAVER M. H., WOSTMANN B. S. Histamine and 5-hydroxytryptamine in the intestinal tract of germ-free animals, animals harbouring one microbial species and conventional animals. Br J Pharmacol Chemother. 1962 Dec;19:385–393. doi: 10.1111/j.1476-5381.1962.tb01443.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHURG J. Renal and renoprival vascular disease in the rat. Arch Pathol. 1963 May;75:547–557. [PubMed] [Google Scholar]
- Carter D., Einheber A. A system for providing surgical anesthesia for germfree rodents. J Appl Physiol. 1965 May;20(3):571–572. doi: 10.1152/jappl.1965.20.3.571. [DOI] [PubMed] [Google Scholar]
- Einheber A., Carter D. The role of the microbial flora in uremia. I. Survival times of germfree, limited-flora, and conventionalized rats after bilateral nephrectomy and fasting. J Exp Med. 1966 Feb 1;123(2):239–250. doi: 10.1084/jem.123.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FLOREY H. W. The secretion and function of intestinal mucus. Gastroenterology. 1962 Sep;43:326–329. [PubMed] [Google Scholar]
- GIORDANO C., BLOOM J., MERRILL J. P. Effects of urea on physiologic systems. I. Studies on monoamine oxidase activity. J Lab Clin Med. 1962 Mar;59:396–400. [PubMed] [Google Scholar]
- KOLFF W. J., FISHER E. R. Pathologic changes after bilateral nephrectomy in dogs and rats. Lab Invest. 1952;1(3):351–363. [PubMed] [Google Scholar]
- KRAMER B., SELIGSON H., BALTRUSH H., SELIGSON D. THE ISOLATION OF SEVERAL AROMATIC ACIDS FROM THE HEMODIALYSIS FLUIDS OF UREMIC PATIENTS. Clin Chim Acta. 1965 Apr;11:363–371. doi: 10.1016/0009-8981(65)90227-5. [DOI] [PubMed] [Google Scholar]
- LEHR D. Causative relationships of parathyroid hormone to renogenic and reniprival cardiovascular disease. Ann N Y Acad Sci. 1959 Jun 16;72:901–969. doi: 10.1111/j.1749-6632.1959.tb44216.x. [DOI] [PubMed] [Google Scholar]
- LEVENSON S. M., CROWLEY L. V., HOROWITZ R. E., MALM O. J. The metabolism of carbon-labeled urea in the germ free rat. J Biol Chem. 1959 Aug;234(8):2061–2062. [PubMed] [Google Scholar]
- LINDSTEDT G., LINDSTEDT S., GUSTAFSSON B. E. MUCUS IN INTESTINAL CONTENTS OF GERMFREE RATS. J Exp Med. 1965 Feb 1;121:201–213. doi: 10.1084/jem.121.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAIFER A., GERSTENFELD S. The photometric microdetermination of blood glucose with glucose oxidase. J Lab Clin Med. 1958 Mar;51(3):448–460. [PubMed] [Google Scholar]
- SALISBURY P. F., POMERANZ A. A. UREMIC TOXICITY CORRELATED WITH UNIDENTIFIED ANIONS. Proc Soc Exp Biol Med. 1963 Nov;114:313–316. doi: 10.3181/00379727-114-28661. [DOI] [PubMed] [Google Scholar]