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. 1966 Feb 28;123(3):433–444. doi: 10.1084/jem.123.3.433

STUDIES ON THE PATHOGENESIS OF FEVER

XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN

Gale W Rafter 1, S Fai Cheuk 1, Donald W Krause 1, W Barry Wood Jr 1
PMCID: PMC2138150  PMID: 5937057

Abstract

Leukocytic pyrogen previously reported to contain an essential protein moiety, appears to be a lipid-protein complex having a molecular weight in the range of 10,000 to 20,000. Evidence that it contains essential lipid includes its inactivation by Cu++, its lability in alkaline solutions (pH 8.5 and above), and its loss of pyrogenicity when extracted with acid-isooctane. Its solubility in 66% methanol, and the enhancing action of ethanol in freeing it from sonicated cells, suggest the presence of exposed lipid groups at its surface. Once the complex is separated from other proteins, its biological activity is readily destroyed. Although the lipid component is presumed to contain unesterified fatty acid(s), its precise composition is unknown. The finding of lipid in the active complex is in keeping with the hypothesis that the pyrogen is derived from leukocytic membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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