Abstract
By combining a tissue culture method with the detection of antibody-producing cells by local hemolysis in gum it has been possible to follow the immunological activity of cells from tissue fragments for long period of time. These fragments were obtained from lymph nodes or spleens of rabbits immunized by sheep erythrocytes. It was found that, while the immunological activity of the free cells in suspensions decreased fast and disappeared in a few days, the cells attaching on glass could express their activity for at least 3 wk. It is assumed that these cells are the daughters of cells from the fragments which were not active antibody producers at the beginning, but differentiated, during the culture, into cells endowed with two capacities: glass adherence and antibody synthesis. One can further admit that the type of culture employed exerts a selective pressure favoring formation of antibody-producing cells.
Full Text
The Full Text of this article is available as a PDF (764.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. I. SINGLE CELL RESPONSES TO SEVERAL ANTIGENS. J Immunol. 1964 Mar;92:335–345. [PubMed] [Google Scholar]
- Bussard A. E., Hannoun C. Examen des immunocytes en culture in vitro: individualisation fonctionnelle et identification morphologique. C R Acad Sci Hebd Seances Acad Sci D. 1965 Jun 14;260(24):6486–6489. [PubMed] [Google Scholar]
- Hannoun C., Bussard A. E. Antibody production by cells in tissue culture. I. Morphological evolution of lymph node and spleen cells in culture. J Exp Med. 1966 Jun 1;123(6):1035–1046. doi: 10.1084/jem.123.6.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- INGRAHAM J. S., BUSSARD A. APPLICATION OF A LOCALIZED HEMOLYSIN REACTION FOR SPECIFIC DETECTION OF INDIVIDUAL ANTIBODY-FORMING CELLS. J Exp Med. 1964 Apr 1;119:667–684. doi: 10.1084/jem.119.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENSEN F. C., GWATKIN R. B., BIGGERS J. D. A SIMPLE ORGAN CULTURE METHOD WHICH ALLOWS SIMULTANEOUS ISOLATION OF SPECIFIC TYPES OF CELLS. Exp Cell Res. 1964 May;34:440–447. doi: 10.1016/0014-4827(64)90231-9. [DOI] [PubMed] [Google Scholar]
- MICHAELIDES M. C., COONS A. H. Studies on antibody production. V. The secondary response in vitro. J Exp Med. 1963 Jun 1;117:1035–1051. doi: 10.1084/jem.117.6.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NETTESHEIM P., MAKINODAN T. DIFFERENTIATION OF LYMPHOCYTES UNDERGOING AN IMMUNE RESPONSE IN DIFFUSION CHAMBERS. J Immunol. 1965 Jun;94:868–876. [PubMed] [Google Scholar]
- NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. IX. THE ANTIGEN CONTENT OF SINGLE ANTIBODY-FORMING CELLS. J Exp Med. 1965 Jun 1;121:945–954. doi: 10.1084/jem.121.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TROWELL O. A. The culture of mature organs in a synthetic medium. Exp Cell Res. 1959 Jan;16(1):118–147. doi: 10.1016/0014-4827(59)90201-0. [DOI] [PubMed] [Google Scholar]