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. 1967 May 1;125(5):863–872. doi: 10.1084/jem.125.5.863

QUANTITATIVE STUDIES OF PHYTOHEMAGGLUTININ-INDUCED DNA AND RNA SYNTHESIS IN NORMAL AND AGAMMAGLOBULINEMIC LEUKOCYTES

Douglass C Tormey 1, Roberta Kamin 1, H Hugh Fudenberg 1
PMCID: PMC2138207  PMID: 6024118

Abstract

Leukocytes from nine patients with acquired agammaglobulinemia were studied in vitro. Synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) induced by phytohemagglutinin was measured by determination of the degree of incorporation of labeled precursor. Synthesis of both DNA and RNA was decreased in the agammaglobulinemic cells. The presence of an inhibitor in the patients' sera could not be demonstrated. These results suggest that the basic defect in agammaglobulinemia is cellular rather than humoral.

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Selected References

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  1. BACH F., HIRSCHHORN K. GAMMA-GLOBULIN PRODUCTION BY HUMAN LYMPHOCYTES IN VITRO. Exp Cell Res. 1963 Dec;32:592–595. doi: 10.1016/0014-4827(63)90197-6. [DOI] [PubMed] [Google Scholar]
  2. BAIN B., VAS M. R., LOWENSTEIN L. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES. Blood. 1964 Jan;23:108–116. [PubMed] [Google Scholar]
  3. BURTIN P., BUFFE D., GRABAR P. LES HYPOGAMMAGLOBULIN'EMIES ATYPIQUES (HYPOGAMMAGLOBULIN'EMIES R'ECESSIVES NON LI'EES AU SEXE). ETUDE IMMUNOCHIMIQUE ET G'EN'ETIQUE. Ann Inst Pasteur (Paris) 1964 Apr;106:519–542. [PubMed] [Google Scholar]
  4. CHARACHE P., ROSEN F. S., JANEWAY C. A., CRAIG J. M., ROSENBERG H. A. ACQUIRED AGAMMAGLOBULINAEMIA IN SIBLINGS. Lancet. 1965 Jan 30;1(7379):234–237. doi: 10.1016/s0140-6736(65)91521-7. [DOI] [PubMed] [Google Scholar]
  5. Chessin L. N., Börjeson J., Welsh P. D., Douglas S. D., Cooper H. L. Studies on human peripheral blood lymphocytes in vitro. II. Morphological and biochemical studies on the transformation of lymphocytes by pokeweed mitogen. J Exp Med. 1966 Nov 1;124(5):873–884. doi: 10.1084/jem.124.5.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cline M. J., Fudenberg H. H. Defective RNA Synthesis in Lymphocytes from Patients with Primary Agammaglobulinemia. Science. 1965 Dec 3;150(3701):1311–1312. doi: 10.1126/science.150.3701.1311. [DOI] [PubMed] [Google Scholar]
  7. Crawford J. P. Side-effects of antiarthritic drugs. Lancet. 1965 Oct 9;2(7415):742–742. doi: 10.1016/s0140-6736(65)90489-7. [DOI] [PubMed] [Google Scholar]
  8. ELVES M. W., ROATH S., ISRAELS M. C. FAILURE OF LYMPHOCYTES FROM HYPOGAMMAGLOBULINAEMIC SUBJECTS TO TRANSFORM IN CULTURE. Br Med J. 1964 Oct 24;2(5416):1051–1052. doi: 10.1136/bmj.2.5416.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EPSTEIN L. B., STOHLMAN F., Jr RNA SYNTHESIS IN CULTURES OF NORMAL HUMAN PERIPHERAL BLOOD. Blood. 1964 Jul;24:69–75. [PubMed] [Google Scholar]
  10. FORBES I. J. SPECIFIC AND NON-SPECIFIC STIMULATION OF ANTIBODY SYNTHESIS BY HUMAN LEUCOCYTES IN VITRO. Lancet. 1965 Jan 23;1(7378):198–199. doi: 10.1016/s0140-6736(65)90979-7. [DOI] [PubMed] [Google Scholar]
  11. FUDENBERG H. H., HIRSCHHORN K. AGAMMAGLOBULINEMIA: THE FUNDAMENTAL DEFECT. Science. 1964 Aug 7;145(3632):611–612. doi: 10.1126/science.145.3632.611. [DOI] [PubMed] [Google Scholar]
  12. FUDENBERG H., GERMAN J. L., 3rd, KUNKEL H. G. The occurrence of rheumatoid factor and other abnormalities in families of patients with agammaglobulinemia. Arthritis Rheum. 1962 Dec;5:565–588. doi: 10.1002/art.1780050605. [DOI] [PubMed] [Google Scholar]
  13. Forbes I. J., Milazzo S. C. A metabolic defect of the lymphocytes in a case of hypogammaglobulinaemia. Australas Ann Med. 1965 Nov;14(4):311–316. doi: 10.1111/imj.1965.14.4.311. [DOI] [PubMed] [Google Scholar]
  14. Fudenberg H. H., Hirschhorn K. Agammaglobulinemia: some current concepts. Med Clin North Am. 1965 Nov;49(6):1533–1552. doi: 10.1016/s0025-7125(16)33245-x. [DOI] [PubMed] [Google Scholar]
  15. HIRSCHHORN K., BACH F., KOLODNY R. L., FIRSCHEIN I. L., HASHEM N. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO. Science. 1963 Nov 29;142(3596):1185–1187. doi: 10.1126/science.142.3596.1185. [DOI] [PubMed] [Google Scholar]
  16. HIRSCHHORN K., SCHREIBMAN R. R., VERBO S., GRUSKIN R. H. THE ACTION OF STREPTOLYSIN S ON PERIPHERAL LYMPHOCYTES OF NORMAL SUBJECTS AND PATIENTS WITH ACUTE RHEUMATIC FEVER. Proc Natl Acad Sci U S A. 1964 Nov;52:1151–1157. doi: 10.1073/pnas.52.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HITZIG W. H., KAY H. E., COTTIER H. FAMILIAL LYMPHOPENIA WITH AGAMMAGLOBULINEMIA: AN ATTEMPT AT TREATMENT BY IMPLANTATION OF FETAL THYMUS. Lancet. 1965 Jul 24;2(7404):151–154. doi: 10.1016/s0140-6736(65)90231-x. [DOI] [PubMed] [Google Scholar]
  18. HITZIG W. H., WILLI H. [Hereditary lymphoplasmocytic dysgenesis ("alymphocytosis with agammaglobulinemia")]. Schweiz Med Wochenschr. 1961 Dec 30;91:1625–1633. [PubMed] [Google Scholar]
  19. LING N. R., SPICER E., JAMES K., WILLIAMSON N. THE ACTIVATION OF HUMAN PERIPHERAL LYMPHOCYTES BY PRODUCTS OF STAPHYLOCOCCI. Br J Haematol. 1965 Jul;11:421–431. doi: 10.1111/j.1365-2141.1965.tb06604.x. [DOI] [PubMed] [Google Scholar]
  20. MACKINNEY A. A., Jr, STOHLMAN F., Jr, BRECHER G. The kinetics of cell proliferation in cultures of human peripheral blood. Blood. 1962 Mar;19:349–358. [PubMed] [Google Scholar]
  21. Mueller G. C., Mahieu M. L. Induction of ribonucleic acid synthesis in human leucocytes by phytohemagglutinin. Biochim Biophys Acta. 1966 Jan 18;114(1):100–107. doi: 10.1016/0005-2787(66)90257-7. [DOI] [PubMed] [Google Scholar]
  22. NOWELL P. C. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960 May;20:462–466. [PubMed] [Google Scholar]
  23. PETERSON R. D., COOPER M. D., GOOD R. A. THE PATHOGENESIS OF IMMUNOLOGIC DEFICIENCY DISEASES. Am J Med. 1965 Apr;38:579–604. doi: 10.1016/0002-9343(65)90135-x. [DOI] [PubMed] [Google Scholar]
  24. PRASAD A. S., ABOUD M. A., SALAWI A., SCHULERT A. R. HYPOGAMMAGLOBULINEMIA; IODINATED GAMMA GLOBULIN STUDIES. Ann Intern Med. 1964 Aug;61:319–325. doi: 10.7326/0003-4819-61-2-319. [DOI] [PubMed] [Google Scholar]
  25. RUECKERT R. R., MUELLER G. C. Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency. Cancer Res. 1960 Dec;20:1584–1591. [PubMed] [Google Scholar]
  26. Rosen F. S., Gotoff S. P., Craig J. M., Ritchie J., Janeway C. A. Further observations on the Swiss type of agammaglobulinemia (alymphocytosis). The effect of syngeneic bone-marrow cells. N Engl J Med. 1966 Jan 6;274(1):18–21. doi: 10.1056/NEJM196601062740104. [DOI] [PubMed] [Google Scholar]
  27. TAO T. W. PHYTOHEMAGGLUTININ ELICITATION OF SPECIFIC ANAMNESTIC IMMUNE RESPONSE IN VITRO. Science. 1964 Oct 9;146(3641):247–248. doi: 10.1126/science.146.3641.247. [DOI] [PubMed] [Google Scholar]
  28. Tormey D. C., Fudenberg H. H., Kamin R. M. Effect of prednisolone on synthesis of DNA and RNA by human lymphocytes in vitro. Nature. 1967 Jan 21;213(5073):281–282. doi: 10.1038/213281a0. [DOI] [PubMed] [Google Scholar]
  29. Tormey D. C., Mueller G. C. An assay for the mitogenic activity of phytohemagglutinin preparations. Blood. 1965 Nov;26(5):569–578. [PubMed] [Google Scholar]
  30. WALDMANN T. A., SCHWAB P. J. IGG (7 S GAMMA GLOBULIN) METABOLISM IN HYPOGAMMAGLOBULINEMIA: STUDIES IN PATIENTS WITH DEFECTIVE GAMMA GLOBULIN SYNTHESIS, GASTROINTESTINAL PROTEIN LOSS, OR BOTH. J Clin Invest. 1965 Sep;44:1523–1533. doi: 10.1172/JCI105259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WEST C. D., HONG R., HOLLAND N. H. Immunoglobulin levels from the newborn period to adulthood and in immunoglobulin deficiency states. J Clin Invest. 1962 Nov;41:2054–2064. doi: 10.1172/JCI104663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. WOLLHEIM F. A., BELFRAGE S., COESTER C., LINDHOLM H. PRIMARY "ACQUIRED" HYPOGAMMAGLOBULINEMIA; CLINICAL AND GENETIC ASPECTS OF NINE CASES. Acta Med Scand. 1964 Jul;176:1–16. [PubMed] [Google Scholar]
  33. YOFFEY J. M. THE LYMPHOCYTE. Annu Rev Med. 1964;15:125–148. doi: 10.1146/annurev.me.15.020164.001013. [DOI] [PubMed] [Google Scholar]

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