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. 1966 Sep 1;124(3):417–429. doi: 10.1084/jem.124.3.417

STUDIES ON THE INDUCTION OF IMMUNOLOGICAL UNRESPONSIVENESS TO PNEUMOCOCCAL POLYSACCHARIDE IN MICE

Gregory W Siskind 1, James G Howard 1
PMCID: PMC2138240  PMID: 4380733

Abstract

1. Comparison of dose-response curves indicated that preimmunized animals were slightly more susceptible to the induction of immunological paralysis with pneumococcal polysaccharide than were normal mice. The results also indicated that the paralysis threshold was unaltered by preimmunization. 2. Transient desensitization of immunized mice could be achieved by an amount of polysaccharide far less than that required to induce paralysis. 3. A transient phase of weak immunity was detected prior to the onset of paralysis when induced by relatively low paralyzing doses of polysaccharide. 4. No "low dose" zone of paralysis (analogous to that obtainable with certain protein antigens) could be elicited with pneumococcal polysaccharide. 5. Massive proliferation of lymphoreticular tissues induced by Corynebacterium parvum failed to raise the threshold for paralysis induction, but amplified the immune response over the entire dose-response curve. Similarly, C. parvum failed to abrogate an established state of paralysis. The results suggest that the induction of polysaccharide paralysis is related to the concentration of antigen in the animal and is not modified by the number of immunologically competent cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENT L., GOWLAND G. Induction of tolerance of skin homografts in immunologically competent mice. Nature. 1962 Dec 29;196:1298–1301. doi: 10.1038/1961298a0. [DOI] [PubMed] [Google Scholar]
  2. Brooke M. S. Conversion of immunological paralysis to immunity by endotoxin. Nature. 1965 May 8;206(984):635–636. doi: 10.1038/206635a0. [DOI] [PubMed] [Google Scholar]
  3. DIETRICH F. M., WEIGLE W. O. IMMUNOLOGIC UNRESPONSIVENESS TO HETEROLOGOUS SERUM PROTEINS INDUCED IN ADULT MICE AND TRANSFER OF THE UNRESPONSIVE STATE. J Immunol. 1964 Feb;92:167–172. [PubMed] [Google Scholar]
  4. DORNER M. M., UHR J. W. IMMUNOLOGIC TOLERANCE AFTER SPECIFIC IMMUNIZATION. J Exp Med. 1964 Sep 1;120:435–447. doi: 10.1084/jem.120.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DORNER M. M., UHR J. W. THE INDUCTION OF IMMUNOLOGICAL TOLERANCE AFTER SPECIFIC ANTIBODY FORMATION. Ann N Y Acad Sci. 1964 Nov 30;120:424–429. doi: 10.1111/j.1749-6632.1964.tb34741.x. [DOI] [PubMed] [Google Scholar]
  6. DRESSER D. W. Specific inhibition of antibody production. I. Protein-over loading paralysis. Immunology. 1962 Jan;5:161–168. [PMC free article] [PubMed] [Google Scholar]
  7. DRESSER D. W. Specific inhibition of antibody production. II. Paralysis induced in adult mice by small quantities of protein antigen. Immunology. 1962 May;5:378–388. [PMC free article] [PubMed] [Google Scholar]
  8. DUTTON R. W. THE EFFECT OF ANTIGEN ON THE PROLIFERATION OF SPLEEN CELL SUSPENSIONS FROM TOLERANT RABBITS. J Immunol. 1964 Nov;93:814–815. [PubMed] [Google Scholar]
  9. FELTON L. D., PRESCOTT B., KAUFFMANN G., OTTINGER B. Pneumococcal antigenic polysaccharide substances from animal tissues. J Immunol. 1955 Mar;74(3):205–213. [PubMed] [Google Scholar]
  10. FELTON L. D. The significance of antigen in animal tissues. J Immunol. 1949 Jan;61(1):107–117. [PubMed] [Google Scholar]
  11. FREI P. C., BENACERRAF B., THORBECKE G. J. PHAGOCYTOSIS OF THE ANTIGEN, A CRUCIAL STEP IN THE INDUCTION OF THE PRIMARY RESPONSE. Proc Natl Acad Sci U S A. 1965 Jan;53:20–23. doi: 10.1073/pnas.53.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FREY J. R., GELEICK H., DEWECK A. IMMUNOLOGICAL TOLERANCE INDUCED IN ANIMALS PREVIOUSLY SENSITIZED TO SIMPLE CHEMICAL COMPOUNDS. Science. 1964 May 15;144(3620):853–854. doi: 10.1126/science.144.3620.853. [DOI] [PubMed] [Google Scholar]
  13. FRIEDMAN H. ADOPTIVE TOLERANCE TO SHIGELLA ANTIGEN IN IRRADIATED MICE RECEIVING SPLEEN CELL TRANSPLANTS FROM UNRESPONSIVE DONORS. J Immunol. 1965 Mar;94:352–357. [PubMed] [Google Scholar]
  14. FRIEDMAN H. Transfer of antibody formation by spleen cells from immunologically unresponsive mice. J Immunol. 1962 Aug;89:257–263. [PubMed] [Google Scholar]
  15. GITLIN D., MONCKEBERG F., JANEWAY C. A. Effects of pneumococcus capsular polysaccarides on the degradation of rabbit antibodies in mice. J Immunol. 1961 Jun;86:627–634. [PubMed] [Google Scholar]
  16. Heidelberger M., Macleod C. M., Kaiser S. J., Robinson B. ANTIBODY FORMATION IN VOLUNTEERS FOLLOWING INJECTION OF PNEUMOCOCCI OR THEIR TYPE-SPECIFIC POLYSACCHARIDES. J Exp Med. 1946 Mar 31;83(4):303–320. [PMC free article] [PubMed] [Google Scholar]
  17. KAPLAN M. E., COONS A. H., DEANE H. W. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse. J Exp Med. 1950 Jan 1;91(1):15-30, 4 pl. doi: 10.1084/jem.91.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MAKELA O., NOSSAL G. J. Accelerated breakdown of immunological tolerance following whole body irradiation. J Immunol. 1962 May;88:613–620. [PubMed] [Google Scholar]
  19. MICHIE D., HOWARD J. G. Transplantation tolerance and immunological immaturity. Ann N Y Acad Sci. 1962 Oct 24;99:670–679. doi: 10.1111/j.1749-6632.1962.tb45350.x. [DOI] [PubMed] [Google Scholar]
  20. Makinodan T., Hoppe I., Sado T., Capalbo E. E., Leonard M. R. The suppressive effect of supraoptimum doses of antigen on the secondary antibody-forming response of spleen cells cultured in cell-impermeable diffusion chambers. J Immunol. 1965 Sep;95(3):466–479. [PubMed] [Google Scholar]
  21. Mäkelä O., Mitchison N. A. The effect of antigen dosage on the response of adoptively transferred cells. Immunology. 1965 Jun;8(6):549–556. [PMC free article] [PubMed] [Google Scholar]
  22. NEEPER C. A., SEASTONE C. V. MECHANISMS OF IMMUNOLOGIC PARALYSIS BY PNEUMOCOCCAL POLYSACCHARIDE. I. STUDIES OF ADOPTIVELY ACQUIRED IMMUNITY TO PNEUMOCOCCAL INFECTION IN IMMUNOLOGICALLY PARALYZED AND NORMAL MICE. J Immunol. 1963 Sep;91:374–377. [PubMed] [Google Scholar]
  23. NEEPER C. A., SEASTONE C. V. MECHANISMS OF IMMUNOLOGIC PARALYSIS BY PNEUMOCOCCAL POLYSACCHARIDE. II. THE INFLUENCE OF NONSPECIFIC FACTORS ON THE IMMUNITY OF PARALYZED MICE TO PNEUMOCOCCAL INFECTION. J Immunol. 1963 Sep;91:378–383. [PubMed] [Google Scholar]
  24. NOSSAL G. J., ADA G. L. RECOGNITION OF FOREIGNNESS IN IMMUNE AND TOLERANT ANIMALS. Nature. 1964 Feb 8;201:580–582. doi: 10.1038/201580a0. [DOI] [PubMed] [Google Scholar]
  25. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. ROWLEY D. A., FITCH F. W. THE MECHANISM OF TOLERANCE PRODUCED IN RATS TO SHEEP ERYTHROCYTES. I. PLAQUE-FORMING CELL AND ANTIBODY RESPONSE TO SINGLE AND MULTIPLE INJECTIONS OF ANTIGEN. J Exp Med. 1965 May 1;121:671–681. doi: 10.1084/jem.121.5.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. ROWLEY D. A., FITCH F. W. THE MECHANISM OF TOLERANCE PRODUCED IN RATS TO SHEEP ERYTHROCYTES. II. THE PLAQUE-FORMING CELL AND ANTIBODY RESPONSE TO MULTIPLE INJECTIONS OF ANTIGEN BEGUN AT BIRTH. J Exp Med. 1965 May 1;121:683–695. doi: 10.1084/jem.121.5.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SERCARZ E. E., COONS A. H. THE ABSENCE OF ANTIBODY-PRODUCING CELLS DURING UNRESPONSIVENESS TO BSA IN THE MOUSE. J Immunol. 1963 Mar;90:478–491. [PubMed] [Google Scholar]
  29. SERCARZ E., COONS A. H. Specific inhibition of antibody formation during immunological paralysis and unresponsiveness. Nature. 1959 Oct 3;184(Suppl 14):1080–1082. doi: 10.1038/1841080a0. [DOI] [PubMed] [Google Scholar]
  30. SISKIND G. W., PATERSON P. Y. A BIOASSAY FOR ESTIMATION OF PNEUMOCOCCAL POLYSACCHARIDE IN UNRESPONSIVE (PARALYZED) MICE. J Immunol. 1964 Sep;93:489–494. [PubMed] [Google Scholar]
  31. SISKIND G. W., PATERSON P. Y., THOMAS L. INDUCTION OF UNRESPONSIVENESS AND IMMUNITY IN NEWBORN AND ADULT MICE WITH PNEUMOCOCCAL POLYSACCHARIDE. J Immunol. 1963 Jun;90:929–934. [PubMed] [Google Scholar]
  32. STASTNY P. PERSISTENCE OF ACQUIRED TOLERANCE IN CELLS TRANSFERRED TO AN ANTIGEN-FREE ENVIRONMENT. J Immunol. 1964 Apr;92:626–629. [PubMed] [Google Scholar]

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