Abstract
Leukemias can be induced in W/Fu inbred rats by neonatal inoculation of normal thymus cells of C58 mice. These leukemias are not transplantable to C58 mice or to adult W/Fu rats, but they can be kept in passage in W/Fu rats aged 0 to 7 days. Adult W/Fu rats inoculated repeatedly with these isogenic leukemias produce cytotoxic and precipitating antibodies. These antisera are of particular value in the analysis of the antigens of leukemia cells and of leukemia viruses because their mode of preparation precludes the formation of antibody against any normal constituents of the cell. Analysis based on the cytotoxic test indicates the presence of 2 distinct cell surface antigens in leukemias induced by Passage A Gross virus or occurring spontaneously in mice of high-incidence strains. All leukemias and other tissues known to contain G (Gross) leukemia antigen have both determinants, but certain leukemias of low-incidence strains have only 1 of them and so were previously classified G-. Immunoprecipitation with these antisera reveals the presence of a cellular antigen common to G+ cells and absent from G- cells; the same antigen can be demonstrated in ether-treated Gross virus, but not in intact virus. This antigen is present also in ether-treated preparations of the Friend, Moloney, and Rauscher leukemia viruses, but not in Bittner (mammary tumor) virus. Thus it may be regarded as a group-specific antigen of murine leukemia viruses, in contrast to the type-specific cellular antigens demonstrable by the cytotoxic test. Four additional antigens associated with leukemias induced by wild-type Gross virus have been demonstrated by immunoprecipitation, but their relation to viral and cellular antigens has not been determined.
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Selected References
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- BOYSE E. A., OLD L. J., STOCKERT E. Some further data on cytotoxic isoantibodies in the mouse. Ann N Y Acad Sci. 1962 Oct 24;99:574–587. doi: 10.1111/j.1749-6632.1962.tb45339.x. [DOI] [PubMed] [Google Scholar]
- FRIEND C. Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med. 1957 Apr 1;105(4):307–318. doi: 10.1084/jem.105.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROSS L. Development and serial cellfree passage of a highly potent strain of mouse leukemia virus. Proc Soc Exp Biol Med. 1957 Apr;94(4):767–771. doi: 10.3181/00379727-94-23080. [DOI] [PubMed] [Google Scholar]
- Huggins C. B., Sugiyama T. Induction of leukemia in rat by pulse doses of 7,12-dimethylbenz(a)anthracene. Proc Natl Acad Sci U S A. 1966 Jan;55(1):74–81. doi: 10.1073/pnas.55.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIM U., CLIFTON K. H., FURTH J. A highly inbred line of Wistar rats yielding spontaneous mammo-somatotropic pituitary and other tumors. J Natl Cancer Inst. 1960 May;24:1031–1055. [PubMed] [Google Scholar]
- KIRSTEN W. H., PLATZ C. E. EARLY AND LATE LEUKEMIAS IN RATS AFTER TRANSPLANTATION OF LEUKEMIC CELLS FROM AKR MICE. Cancer Res. 1964 Jul;24:1056–1062. [PubMed] [Google Scholar]
- MOLONEY J. B. Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations. J Natl Cancer Inst. 1960 Apr;24:933–951. [PubMed] [Google Scholar]
- OLD L. J., BOYSE E. A., STOCKERT E. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS. Nature. 1964 Feb 22;201:777–779. doi: 10.1038/201777a0. [DOI] [PubMed] [Google Scholar]
- Old L. J., Boyse E. A. Antigens of tumors and leukemias induced by viruses. Fed Proc. 1965 Sep-Oct;24(5):1009–1017. [PubMed] [Google Scholar]
- RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
- SLETTENMARK B., KLEIN E. Cytotoxic and neutralization tests with serum and lymph node cells of isologous mice with induced resistance against gross lymphomas. Cancer Res. 1962 Sep;22:947–954. [PubMed] [Google Scholar]
- STUECK B., OLD L. J., BOYSE E. A. OCCURRENCE OF SOLUBLE ANTIGEN IN THE PLASMA OF MICE WITH VIRUS-INDUCED LEUKEMIA. Proc Natl Acad Sci U S A. 1964 Oct;52:950–958. doi: 10.1073/pnas.52.4.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAHREN B. COMPARISON OF TUMOUR ANTIGENS IN AKR AND GROSS VIRUS (PASSAGE A)-INDUCED LEUKAEMIAS. Nature. 1965 Jan 23;205:409–410. doi: 10.1038/205409a0. [DOI] [PubMed] [Google Scholar]