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. 1966 Oct 1;124(4):773–785. doi: 10.1084/jem.124.4.773

DEFICIENCY OF THE SIXTH COMPONENT OF COMPLEMENT IN RABBITS WITH AN INHERITED COMPLEMENT DEFECT

Klaus Rother 1, Ursula Rother 1, Hans J Müller-Eberhard 1, Ulf R Nilsson 1
PMCID: PMC2138255  PMID: 5922290

Abstract

A strain of rabbits with an inherited complement deficiency was shown to lack the sixth component of the hemolytic complement system. A method was elaborated for the partial purification of this component from normal rabbit serum. Upon injection of partially purified rabbit C'6 into C'6-deficient animals, an antibody was obtained which specifically inhibited the hemolytic activity of C'6. The data suggest that C'6-deficient serum either lacks the C'6 molecule or contains it in a chemically modified and inactive form.

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Selected References

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  1. CINADER B., DUBISKI S., WARDLAW A. C. DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT. J Exp Med. 1964 Nov 1;120:897–924. doi: 10.1084/jem.120.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. MARDINEY M. R., Jr, MUELLER-EBERHARD H. J. MOUSE BETA-1C-GLOBULIN: PRODUCTION OF ANTISERUM AND CHARACTERIZATION IN THE COMPLEMENT REACTION. J Immunol. 1965 Jun;94:877–882. [PubMed] [Google Scholar]
  3. MULLER-EBERHARD H. J. A new supporting medium for preparative electrophoresis. Scand J Clin Lab Invest. 1960;12:33–37. [PubMed] [Google Scholar]
  4. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  5. NILSSON U. R., MUELLER-EBERHARD H. J. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 Aug 1;122:277–298. doi: 10.1084/jem.122.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. NISHIOKA K., LINSCOTT W. D. COMPONENTS OF GUINEA PIG COMPLEMENT. I. SEPARATION OF A SERUM FRACTION ESSENTIAL FOR IMMUNE HEMOLYSIS AND IMMUNE ADHERENCE. J Exp Med. 1963 Nov 1;118:767–793. doi: 10.1084/jem.118.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
  8. POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
  9. ROSENBERG L. T., TACHIBANA D. K. Activity of mouse complement. J Immunol. 1962 Dec;89:861–867. [PubMed] [Google Scholar]
  10. ROTHER K., ROTHER U., SCHINDERA F. PASSIVE ARTHUS-REAKTION BEI KOMPLEMENT-DEFEKTEN KANINCHEN. Z Immunitats Allergieforsch. 1964 Jun;126:473–488. [PubMed] [Google Scholar]
  11. Rother K., Rother U. Studies on complement defective rabbits. IV. Blood clearance of intravenously injected S. typhi by the reticuloendothelial system. Proc Soc Exp Biol Med. 1965 Aug-Sep;119(4):1055–1059. doi: 10.3181/00379727-119-30375. [DOI] [PubMed] [Google Scholar]
  12. SMITHIES O. Zone electrophoresis in starch gels and its application to studies of serum proteins. Adv Protein Chem. 1959;14:65–113. doi: 10.1016/s0065-3233(08)60609-9. [DOI] [PubMed] [Google Scholar]
  13. UNANUE E., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. IV. PARTICIPATION OF COMPLEMENT IN NEPHROTOXIC NEPHRITIS. J Exp Med. 1964 Jan 1;119:965–982. doi: 10.1084/jem.119.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. VOLK H., MAUERSBERGER D., ROTHER K., ROTHER U. PROLONGED SURVIVAL OF SKIN HOMOGRAFTS IN RABBITS DEFECTIVE IN THE THIRD COMPONENT OF COMPLEMENT. Ann N Y Acad Sci. 1964 Nov 30;120:26–27. doi: 10.1111/j.1749-6632.1964.tb34703.x. [DOI] [PubMed] [Google Scholar]
  15. WARD P. A., COCHRANE C. G., MUELLER-EBERHARD H. J. THE ROLE OF SERUM COMPLEMENT IN CHEMOTAXIS OF LEUKOCYTES IN VITRO. J Exp Med. 1965 Aug 1;122:327–346. doi: 10.1084/jem.122.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ward P. A., Cochrane C. G., Muller-Eberhard H. J. Further studies on the chemotactic factor of complement and its formation in vivo. Immunology. 1966 Aug;11(2):141–153. [PMC free article] [PubMed] [Google Scholar]

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