Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1966 Oct 31;124(5):787–803. doi: 10.1084/jem.124.5.787

PRESENCE OF TWO TYPES OF L POLYPEPTIDE CHAINS IN GUINEA PIG 7S IMMUNOGLOBULINS

Victor Nussenzweig 1, Michael E Lamm 1, Baruj Benacerraf 1
PMCID: PMC2138270  PMID: 4959010

Abstract

Guinea pig 7S immunoglobulins (γ1 and γ2) consist of two groups of molecules (K and L) bearing different types of L chains (κ and λ). Approximately one-third of the molecules in normal guinea pig γ2-globulin bear the λ- type of L chains, and all or most of the others bear κ-chains. κ- and λ-chains have similar molecular weights.

Full Text

The Full Text of this article is available as a PDF (921.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernier G. M., Cebra J. J. Frequency distribution of alpha, gamma, kappa and lambda polypeptide chains in human lymphoid tissues. J Immunol. 1965 Aug;95(2):246–253. [PubMed] [Google Scholar]
  3. COHEN S., PORTER R. B. STRUCTURE AND BIOLOGICAL ACTIVITY OF IMMUNOGLOBULINS. Adv Immunol. 1964;27:287–349. doi: 10.1016/s0065-2776(08)60710-5. [DOI] [PubMed] [Google Scholar]
  4. EDELMAN G. M., BENACERRAF B., OVARY Z., POULIK M. D. Structural differences among antibodies of different specificities. Proc Natl Acad Sci U S A. 1961 Nov 15;47:1751–1758. doi: 10.1073/pnas.47.11.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EDELMAN G. M., OLINS D. E., GALLY J. A., ZINDER N. D. RECONSTITUTION OF IMMUNOLOGIC ACTIVITY BY INTERACTION OF POLYPEPTIDE CHAINS OF ANTIBODIES. Proc Natl Acad Sci U S A. 1963 Oct;50:753–761. doi: 10.1073/pnas.50.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  7. FAHEY J. L. TWO TYPES OF 6.6 S GAMMA-GLOBULINS, BETA-2A-GLOBULINS AND 18 S GAMMA 1-MACROGLOBULINS IN NORMAL SERUM AND GAMMA-MICROGLOBULINS IN NORMAL URINE. J Immunol. 1963 Oct;91:438–447. [PubMed] [Google Scholar]
  8. FEINSTEIN A. ELECTROPHORETIC BEHAVIOUR OF RABBIT GAMMA-GLOBULIN FRAGMENTS AND CHAINS. Nature. 1964 Jun 27;202:1298–1301. doi: 10.1038/2021298a0. [DOI] [PubMed] [Google Scholar]
  9. Fudenberg H. H. The immune globulins. Annu Rev Microbiol. 1965;19:301–338. doi: 10.1146/annurev.mi.19.100165.001505. [DOI] [PubMed] [Google Scholar]
  10. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hilschmann N., Craig L. C. Amino acid sequence studies with Bence-Jones proteins. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1403–1409. doi: 10.1073/pnas.53.6.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hong R., Nisonoff A. Heterogeneity in the complementation of polypeptide subunits of a purified antibody isolated from an individual rabbit. J Immunol. 1966 Apr;96(4):622–628. [PubMed] [Google Scholar]
  13. Lamm M. E., Nussenzweig V., Benacerraf B. Isolation of purified H and L polypeptide chains from guinea-pig gamma-2-immunoglobulin after mild reduction. Immunology. 1966 Apr;10(4):309–319. [PMC free article] [PubMed] [Google Scholar]
  14. MULLER-EBERHARD H. J., KUNKEL H. G. The carbohydrate of gamma-globulin and myeloma proteins. J Exp Med. 1956 Aug 1;104(2):253–269. doi: 10.1084/jem.104.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mannik M., Kunkel H. G. TWO MAJOR TYPES OF NORMAL 7S gamma-GLOBULIN. J Exp Med. 1963 Jan 31;117(2):213–230. doi: 10.1084/jem.117.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. NUSSENZWEIG V., BENACERRAF B. DIFFERENCES IN THE ELECTROPHORETIC MOBILITIES OF GUINEA PIG 7S ANTIBODIES OF DIFFERENT SPECIFICITIES. J Exp Med. 1964 Mar 1;119:409–423. doi: 10.1084/jem.119.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nussenzweig V., Benacerraf B. Presence of identical antigenic determinants in the Fd fragments of gamma-1- and gamma-2-guinea pig immunoglobulins. J Immunol. 1966 Aug;97(2):171–176. [PubMed] [Google Scholar]
  18. Nussenzweig V., Benacerraf B. Quantitative variations in L chain types in guinea pig antihapten antibodies. J Exp Med. 1966 Nov 1;124(5):805–818. doi: 10.1084/jem.124.5.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. POTTER M., DREYER W. J., KUFF E. L., MCINTIRE K. R. HERITABLE VARIATION IN BENCE JONES PROTEIN STRUCTURE IN AN INBRED STRAIN OF MICE. J Mol Biol. 1964 Jun;8:814–822. doi: 10.1016/s0022-2836(64)80162-5. [DOI] [PubMed] [Google Scholar]
  20. ROHOLT O., ONOUE K., PRESSMAN D. SPECIFIC COMBINATION OF H AND L CHAINS OF RABBIT GAMMA-GLOBULINS. Proc Natl Acad Sci U S A. 1964 Feb;51:173–178. doi: 10.1073/pnas.51.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SCHWARTZ J. H., EDELMAN G. M. Comparisons of Bence-Jones proteins and L polypeptide chains of myeloma globulins after hydrolysis with trypsin. J Exp Med. 1963 Jul;118:41–53. doi: 10.1084/jem.118.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. THORBECKE G. J., BENACERRAF B., OVARY Z. ANTIGENIC RELATIONSHIP BETWEEN TWO TYPES OF 7 S GUINEA PIG GAMMA-GLOBULINS. J Immunol. 1963 Nov;91:670–676. [PubMed] [Google Scholar]
  23. Titani K., Whitley E., Jr, Avogardo L., Putnam F. W. Immunoglobulin structure: partial amino acid sequence of a Bence Jones protein. Science. 1965 Sep 3;149(3688):1090–1092. doi: 10.1126/science.149.3688.1090. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES