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. 1966 Oct 31;124(5):833–849. doi: 10.1084/jem.124.5.833

MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES

VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT

Winnifred Seegers 1, Aaron Janoff 1
PMCID: PMC2138271  PMID: 5926298

Abstract

The mast cell-rupturing component present in the lysosomes of rabbit exudate PMN neutrophil leukocytes has been identified and some of its physical and chemical properties have been described. The active agent is a low molecular weight (1200 to 2400) polypeptide containing a relatively large proportion of the basic amino acid, arginine. It is thermostable and dialyzable, and does not cause contraction of the isolated guinea pig ileum. The mast cell-rupturing activity of the agent is destroyed by trypsin. A second permeability factor with a larger molecular weight is present in crude extracts of PMN granules. Although this substance does not lyse mast cells, it is capable of evoking delayed permeability responses in rabbit skin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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