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. 1966 Oct 31;124(5):1001–1016. doi: 10.1084/jem.124.5.1001

PRIMARY ANTIBODY RESPONSE IN ORGAN CULTURES

Amiela Globerson 1, Robert Auerbach 1
PMCID: PMC2138273  PMID: 5926296

Abstract

Specific antibody formation has been elicited in vitro following antigenic stimulation by either sheep (in a total of 472 of 875 cultures) or chick erythrocytes (in 65 of 135 cultures tested). The response was manifested by mouse spleen and lymph node explants whereas thymus cultures were inactive. The reaction has been characterized as a primary immune response in view of its kinetics as compared to defined primary and secondary responses, the effect of 2-mercaptoethanol on the antibodies formed, its subject to puromycin inhibition and its sensitivity to X-irradiation. Histological studies revealed preservation of the lymphoid cell populations throughout the entire experimental period.

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Selected References

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  1. ASKONAS B. A., RHODES J. M. IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES. Nature. 1965 Jan 30;205:470–474. doi: 10.1038/205470a0. [DOI] [PubMed] [Google Scholar]
  2. ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. IV. THE DETAILED METHODS FOR MEASURING ANTIBODY SYNTHESIS BY INDIVIDUAL CELLS, THE KINETICS OF ANTIBODY FORMATION BY RABBITS AND THE PROPERTIES OF CELL SUSPENSIONS. J Immunol. 1964 Mar;92:372–390. [PubMed] [Google Scholar]
  3. AUERBACH R. Morphogenetic interactions in the development of the mouse thymus gland. Dev Biol. 1960 Jun;2:271–284. doi: 10.1016/0012-1606(60)90009-9. [DOI] [PubMed] [Google Scholar]
  4. Bauer D. C., Stavitsky A. B. ON THE DIFFERENT MOLECULAR FORMS OF ANTIBODY SYNTHESIZED BY RABBITS DURING THE EARLY RESPONSE TO A SINGLE INJECTION OF PROTEIN AND CELLULAR ANTIGENS. Proc Natl Acad Sci U S A. 1961 Oct;47(10):1667–1680. doi: 10.1073/pnas.47.10.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carrel A., Ingebrigtsen R. THE PRODUCTION OF ANTIBODIES BY TISSUES LIVING OUTSIDE OF THE ORGANISM. J Exp Med. 1912 Mar 1;15(3):287–291. doi: 10.1084/jem.15.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEUTSCH H. F., MORTON J. I. Human serum macroglobulins and dissociation units. I. Physicochemical properties. J Biol Chem. 1958 Apr;231(2):1107–1118. [PubMed] [Google Scholar]
  7. FISHMAN M., ADLER F. L. Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen. J Exp Med. 1963 Apr 1;117:595–602. doi: 10.1084/jem.117.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FISHMAN M. Antibody formation in tissue culture. Nature. 1959 Apr 25;183(4669):1200–1201. doi: 10.1038/1831200a0. [DOI] [PubMed] [Google Scholar]
  9. FISHMAN M., HAMMERSTROM R. A., BOND V. P. In vitro transfer of macrophage RNA to lymph node cells. Nature. 1963 May 11;198:549–551. doi: 10.1038/198549a0. [DOI] [PubMed] [Google Scholar]
  10. FRIEDMAN H. ANTIBODY PLAQUE FORMATION BY NORMAL MOUSE SPLEEN CELL CULTURES EXPOSED IN VITRO TO RNA FROM IMMUNE MICE. Science. 1964 Nov 13;146(3646):934–936. doi: 10.1126/science.146.3646.934. [DOI] [PubMed] [Google Scholar]
  11. Friedman H. P., Stavitsky A. B., Solomon J. M. Induction in vitro of antibodies to phage T2: antigens in the RNA extract employed. Science. 1965 Sep 3;149(3688):1106–1107. doi: 10.1126/science.149.3688.1106. [DOI] [PubMed] [Google Scholar]
  12. Globerson A., Auerbach R. Primary immune reactions in organ cultures. Science. 1965 Aug 27;149(3687):991–993. doi: 10.1126/science.149.3687.991. [DOI] [PubMed] [Google Scholar]
  13. Globerson A. In vitro studies on radiation lymphoid recovery of mouse spleen. J Exp Med. 1966 Jan 1;123(1):25–32. doi: 10.1084/jem.123.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nakano M., Braun W. Fluctuation tests with antibody-forming spleen cell populations. Science. 1966 Jan 21;151(3708):338–340. doi: 10.1126/science.151.3708.338. [DOI] [PubMed] [Google Scholar]
  16. Parker R. C. STUDIES ON THE PRODUCTION OF ANTIBODIES IN VITRO. Science. 1937 Mar 19;85(2203):292–294. doi: 10.1126/science.85.2203.292-a. [DOI] [PubMed] [Google Scholar]
  17. SCHOENBERG M. D., MUMAW V. R., MOORE R. D., WEISBERGER A. S. CYTOPLASMIC INTERACTION BETWEEN MACROPHAGES AND LYMPHOCYTIC CELLS IN ANTIBODY SYNTHESIS. Science. 1964 Feb 28;143(3609):964–965. doi: 10.1126/science.143.3609.964. [DOI] [PubMed] [Google Scholar]
  18. Saunders G. C., King D. W. Antibody synthesis initiated in vitro by paired explants of spleen and thymus. Science. 1966 Mar 18;151(3716):1390–1391. doi: 10.1126/science.151.3716.1390. [DOI] [PubMed] [Google Scholar]
  19. TALIAFERRO W. H., TALIAFERRO L. G., JANSSEN E. F. The localization of x-ray injury to the initial phases of antibody response. J Infect Dis. 1952 Sep-Oct;91(2):105–124. doi: 10.1093/infdis/91.2.105. [DOI] [PubMed] [Google Scholar]
  20. Tao T. W., Uhr J. W. Primary-type antibody response in vitro. Science. 1966 Mar 4;151(3714):1096–1098. doi: 10.1126/science.151.3714.1096. [DOI] [PubMed] [Google Scholar]
  21. WHEELOCK E. F. The role of protein synthesis in the eclipse period of newcastle disease virus multiplication in HeLa cells as studied with puromycin. Proc Natl Acad Sci U S A. 1962 Aug;48:1358–1366. doi: 10.1073/pnas.48.8.1358. [DOI] [PMC free article] [PubMed] [Google Scholar]

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