Abstract
Genetic nonresponder guinea pigs incapable of an immune response to DNP-PLL alone were immunized with DNP-PLL complexed to ovalbumin or bovine serum albumin. Under these circumstances the animals produce both anti-DNP-PLL antibodies and antibodies directed against the conveyor albumin. Thus immune response to DNP-PLL complexed to conveyor albumin molecules can serve as a simple model of hapten-carrier relationships. To explore these relationships the question whether these two types of antibody are synthesized by the same or by different plasma cells was investigated by a combination of a double immunofluorescent technique and radioautographic localization of radioactive antigen. It was shown that the anti-DNP-PLL antibodies and the antibodies against the carrier albumin molecule were produced in separate cells. No cell-producing antibodies with both specificities were detected out of 526 cells studied.
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Selected References
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- ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. I. SINGLE CELL RESPONSES TO SEVERAL ANTIGENS. J Immunol. 1964 Mar;92:335–345. [PubMed] [Google Scholar]
- ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. II. FURTHER OBSERVATIONS ON THE BEHAVIOR OF SINGLE ANTIBODY-PRODUCING CELLS WITH RESPECT TO THEIR SYNTHETIC CAPACITY AND MORPHOLOGY. J Immunol. 1964 Mar;92:346–355. [PubMed] [Google Scholar]
- ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS.III. THE CONTROLS FOR MICRODROP AND MICROPIPET EXPERIMENTS. J Immunol. 1964 Mar;92:356–371. [PubMed] [Google Scholar]
- BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COONS A. H., LEDUC E. H., CONNOLLY J. M. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955 Jul 1;102(1):49–60. doi: 10.1084/jem.102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GELL P. G., SILVERSTEIN A. M. Delayed hypersensitivity to hapten-protein conjugates. I. The effect of carrier protein and site of attachment to hapten. J Exp Med. 1962 May 1;115:1037–1051. doi: 10.1084/jem.115.5.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green I., Paul W. E., Benacerraf B. The behavior of hapten-poly-L-lysine conjugates as complete antigens in genetic responder and as haptens in nonresponder guinea pigs. J Exp Med. 1966 May 1;123(5):859–879. doi: 10.1084/jem.123.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green I., Vassalli P., Nussenzweig V., Benacerraf B. Specificity of the antibodies produced by single cells following immunization with antigens bearing two types of antigenic determinants. J Exp Med. 1967 Mar 1;125(3):511–526. doi: 10.1084/jem.125.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
- LEVINE B. B., BENACERRAF B. GENETIC CONTROL IN GUINEA PIGS OF IMMUNE RESPONSE TO CONJUGATES OF HAPTENS AND POLY-L-LYSINE. Science. 1965 Jan 29;147(3657):517–518. doi: 10.1126/science.147.3657.517. [DOI] [PubMed] [Google Scholar]
- LEVINE B. B., OJEDA A., BENACERRAF B. STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS. J Exp Med. 1963 Dec 1;118:953–957. doi: 10.1084/jem.118.6.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OVARY Z., BENACERRAF B. IMMUNOLOGICAL SPECIFICITY OF THE SECONDARY RESPONSE WITH DINITROPHENYLATED PROTEINS. Proc Soc Exp Biol Med. 1963 Oct;114:72–76. doi: 10.3181/00379727-114-28589. [DOI] [PubMed] [Google Scholar]
- Paul W. E., Siskind G. W., Benacerraf B. Studies on the effect of the carrier molecule on antihapten antibody synthesis. II. Carrier specificity of anti-2,4-dinitrophenyl-poly-l-lysine antibodies. J Exp Med. 1966 Apr 1;123(4):689–705. doi: 10.1084/jem.123.4.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. The specificity of allergic reactions. I. Delayed versus Arthus hypersensitivity. J Exp Med. 1960 Apr 1;111:465–483. doi: 10.1084/jem.111.4.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlossman S. F., Ben-Efraim S., Yaron A., Sober H. A. Immunochemical studies on the antigenic determinants required to elicit delayed and immediate hypersensitivity reactions. J Exp Med. 1966 Jun 1;123(6):1083–1095. doi: 10.1084/jem.123.6.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlossman S. F., Yaron A., Ben-Efraim S., Sober H. A. Immunogenicity of a series of alpha,N-DNP-L-lysines. Biochemistry. 1965 Aug;4(8):1638–1645. doi: 10.1021/bi00884a028. [DOI] [PubMed] [Google Scholar]