Abstract
To determine whether the factor(s) responsible for the conservation of epidermal specificities in adult guinea pigs and hamsters resides in the germinal layer of the epidermis or in the dermis, thin grafts of skin, possessing qualitatively distinct regional characteristics, were separated into their superficial epidermal and dermal components with the aid of trypsin. Dermis of one type was combined with epidermis of another to produce "recombinant" grafts which were then transplanted to small, full thickness cutaneous sites on the thorax of geneticaily compatible hosts. A variant of this procedure involved transplanting sheets of superficial epidermis of various types to shallow split thickness recipient areas in the skin of the thorax. All grafts were maintained for 100 days before they were excised and examined histologically. The results indicate that, whereas the dermis determines the kind of epidermis produced in recombinant grafts involving the ear, the sole of the foot, and the trunk, this is not the case in recombinants which include tongue, esophageal, or cheek pouch epithelia. The one exception to this occurred when tongue or esophagus epithelia were transplanted to split thickness beds in trunk skin. Here they appeared to produce an epidermis characteristic of their new location. It is believed that this exception is probably due to the fact that the native follicular epidermis present in trunk dermis made such a substantial contribution to the new superficial epidermis that it behaved overtly as body skin epidermis. Taken together, these results suggest that basal layer cells of the superficial epidermis of sole of foot skin, ear skin, and the hair-bearing skin of the general integument behave as if they are equipotential, and that in adult life maintenance of these particular epidermal specificities is the outcome of persistent specific inductive stimuli from the underlying dermis. The results of subsidiary experiments are reported which indicate that the epithelial component of mammary gland tissue is also pluripotential, being capable of producing, under appropriate conditions, a normal-looking, fully stratified superficial epidermis.
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Selected References
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- Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures. J Cell Biol. 1966 Mar;28(3):473–487. doi: 10.1083/jcb.28.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BILLINGHAM R. E., SILVERS W. K. The melanocytes of mammals. Q Rev Biol. 1960 Mar;35:1–40. doi: 10.1086/402951. [DOI] [PubMed] [Google Scholar]
- BILLINGHAM R. E., SILVERS W. K. The origin and conservation of epidermal specificities. N Engl J Med. 1963 Mar 7;268:539–concl. doi: 10.1056/NEJM196303072681008. [DOI] [PubMed] [Google Scholar]
- BREEDIS C. Regeneration of hair follicles and sebaceous glands from the epithelium of scars in the rabbit. Cancer Res. 1954 Sep;14(8):575–579. [PubMed] [Google Scholar]
- Billingham R. E., Medawar P. B. A note on the specificity of the corneal epithelium. J Anat. 1950 Jan;84(Pt 1):50–56. [PMC free article] [PubMed] [Google Scholar]
- LEBLOND C. P., WALKER B. E. Renewal of cell populations. Physiol Rev. 1956 Apr;36(2):255–276. doi: 10.1152/physrev.1956.36.2.255. [DOI] [PubMed] [Google Scholar]
- Oliver R. F. Whisker growth after removal of the dermal papilla and lengths of follicle in the hooded rat. J Embryol Exp Morphol. 1966 Jun;15(3):331–347. [PubMed] [Google Scholar]
- Ramseier H., Billingham R. E. Studies on delayed cutaneous inflammatory reactions elicited by inoculation of homologous cells into hamsters' skins. J Exp Med. 1966 Apr 1;123(4):629–656. doi: 10.1084/jem.123.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silvers W. K., Billingham R. E. Further studies on the induction of tolerance of skin homografts in rats. J Exp Zool. 1966 Apr;161(3):413–423. doi: 10.1002/jez.1401610308. [DOI] [PubMed] [Google Scholar]
- VAN SCOTT E. J., REINERTSON R. P. The modulating influence of stromal environment on epithelial cells studied in human autotransplants. J Invest Dermatol. 1961 Feb;36:109–131. [PubMed] [Google Scholar]
- WESSELLS N. K. Tissue interactions during skin histodifferentiation. Dev Biol. 1962 Feb;4:87–107. doi: 10.1016/0012-1606(62)90034-9. [DOI] [PubMed] [Google Scholar]