Abstract
Infectious laryngotracheitis can be produced in chickens as an experimental model of severe nonfatal rhinitis and sinusitis. Inoculated intranasally into unanesthetized baby chicks it remains limited to the nasal fossa, produces acute desquamation of all nasal epithelia, results in functional recovery of the respiratory epithelium, but leaves important residual abnormalities. From the earliest recognizable lesions through 4½ months' convalescence, the principal changes are as follows: 1. Initial lesions, or small syncytia of intranuclear "inclusions", first identifiable in the mucociliated cells of the shallowest portion of the epithelium at about 21 hr postinoculum (the inner surface of the maxillary conchal scroll). 2. Acute sloughing, (about 3 to 7 days), marked by: (a) spread of lesions from cell to cell via multinucleated "giant cells" which progressively slough and desquamate respiratory, olfactory, and sinus epithelia, epithelial neural elements and blood vessels; (b) appearance of numbers of eosinophilic leukocytes along the basement membrane at the sites of lesions just previous to sloughing; intensive infiltration of the submucosa with small lymphocytes after sloughing begins; (c) histochemical change in the intracellular mucus of the cells which comprise the syncytia: this mucus stains with Alcian blue alone when stained with AB-PAS; and (d) all cartilages of the maxillary conchae become flaccid, and the cell nuclei and matrix lose both basophilic and Alcian blue staining properties, effects which recede by about the 8th day. 3. Repair (about 8 to 21 days), marked by rapid initial spread of a sheet of epithelial cells over the infiltrated subrmucosa, appearance of numbers of plasma cells circulating in the tissues, formation of encapsulated secondary nodules, and mucosal adhesions. 4. Convalescence (about 1 to 4½ months when experiments terminated), marked by functional restoration of the mucociliary lining of the nasal fossa. However, at 4½ months eight specimens all show complete metaplasia of the olfactory organ (end nerves, supporting cells, and glands of Bowman) to mucociliated epithelium, all show abnormal formation and alignment of mucous acini, and about 50% have severe persistent sinusitis.
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Selected References
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- BANG B. G., BANG F. B. A comparative study of the vertebrate nasal chamber in relation to upper respiratory infections. Bull Johns Hopkins Hosp. 1959 Mar;104(3):107–149. [PubMed] [Google Scholar]
- BANG B. G. THE MUCOUS GLANDS OF THE DEVELOPING HUMAN NOSE. Acta Anat (Basel) 1964;59:297–314. doi: 10.1159/000142625. [DOI] [PubMed] [Google Scholar]
- BANG B. G. The surface pattern of the nasal mucosa and its relation to mucous flow--a study of chicken and herring gull nasal mucosae. J Morphol. 1961 Jul;109:57–71. doi: 10.1002/jmor.1051090105. [DOI] [PubMed] [Google Scholar]
- BANG F. B., FOARD M. INTERACTION OF RESPIRATORY EPITHELIUM OF THE CHICK AND NEWCASTLE DISEASE VIRUS. Am J Hyg. 1964 May;79:260–278. doi: 10.1093/oxfordjournals.aje.a120382. [DOI] [PubMed] [Google Scholar]
- BOJSEN-MOLLER F. GLANDULAE NASALES ANTERIORES IN THE HUMAN NOSE. Ann Otol Rhinol Laryngol. 1965 Jun;74:363–375. doi: 10.1177/000348946507400207. [DOI] [PubMed] [Google Scholar]
- BURNSTEIN T., BANG F. B. Infection of the upper respiratory tract of the chick with a mild (vaccine) strain of Newcastle disease virus: I. Initiation and spread of the infection. Bull Johns Hopkins Hosp. 1958 Mar;102(3):127–134. [PubMed] [Google Scholar]
- Beach J. R. A BACTERIOLOGICAL STUDY OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS. J Exp Med. 1931 Nov 30;54(6):801–808. doi: 10.1084/jem.54.6.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COATES H. V., CHANOCK R. M. Experimental infection with respiratory syncytial virus in several species of animals. Am J Hyg. 1962 Nov;76:302–312. doi: 10.1093/oxfordjournals.aje.a120285. [DOI] [PubMed] [Google Scholar]
- FELL H. B., THOMAS L. Comparison of the effects of papain and vitamin A on cartilage. II. The effects on organ cultures of embryonic skeletal tissue. J Exp Med. 1960 May 1;111:719–744. doi: 10.1084/jem.111.5.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Francis T., Stuart-Harris C. H. STUDIES ON THE NASAL HISTOLOGY OF EPIDEMIC INFLUENZA VIRUS INFECTION IN THE FERRET : I. THE DEVELOPMENT AND REPAIR OF THE NASAL LESION. J Exp Med. 1938 Oct 31;68(6):789–802. doi: 10.1084/jem.68.6.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GROBSTEIN C. CYTODIFFERENTIATION AND ITS CONTROLS. Science. 1964 Feb 14;143(3607):643–650. doi: 10.1126/science.143.3607.643. [DOI] [PubMed] [Google Scholar]
- Goodpasture E. W. The Axis-Cylinders of Peripheral Nerves as Portals of Entry to the Central Nervous System for the Virus of Herpes Simplex in Experimentally Infected Rabbits. Am J Pathol. 1925 Jan;1(1):11–28.5. [PMC free article] [PubMed] [Google Scholar]
- HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
- HOTZ G., BANG F. B. Electron microscope studies of ferret respiratory cells infected with influenza. Bull Johns Hopkins Hosp. 1957 Oct;101(4):175–208. [PubMed] [Google Scholar]
- SPICER S. S. Histochemical differentiation of mammalian macopolysaccharides. Ann N Y Acad Sci. 1963 Mar 30;106:379–388. doi: 10.1111/j.1749-6632.1963.tb16652.x. [DOI] [PubMed] [Google Scholar]
- Seifried O. HISTOPATHOLOGY OF INFECTIOUS LARYNGOTRACHEITIS IN CHICKENS. J Exp Med. 1931 Nov 30;54(6):817–826. doi: 10.1084/jem.54.6.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THOMAS L., McCLUSKEY R. T., POTTER J. L., WEISSMANN G. Comparison of the effects of papain n vitamin A on cartilage. I. The effects in rabbits. J Exp Med. 1960 May 1;111:705–718. doi: 10.1084/jem.111.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]