Abstract
A study has been conducted on the influence of nucleosides and nucleotides to induce the formation of pinocytic vesicles in cultured mouse macrophages. Extremely high levels of cytoplasmic vesicles were found after exposure of macrophages to adenosine 5'-phosphate, ADP, and ATP. A limited vesicle response was obtained with adenosine 2'-. and 3'-phosphate, 3',5'-adenosine-phosphate, and deoxyadenosine 5'-phosphate. The di- and triphosphates of guanosine, inosine, cytidine, and uridine were stimulatory but much less active than the adenosine derivatives. Adenosine administration resulted in high levels of pinocytic activity whereas other nucleosides, including inosine, guanosine, cytidine, and uridine, yielded little or no stimulatory effect. Adenosine and its 5'-phosphates produced morphological effects on macrophages characterized by increased spreading, a thin, peripheral cytoplasmic veil with denser cores of oriented mitochondria and contraction of the centre-sphere region. Large numbers of pinosomes were seen in association with mitochondria-containing portions of the cytoplasm. The stimulatory effects of adenosine and ATP were rapid and involved the majority of cells in the culture. Adenosine was unable to reverse the pinocytosis inhibition produced by 2,4-DNP, whereas ATP raised vesicle counts to high levels. Possible mechanisms for these effects are discussed.
Full Text
The Full Text of this article is available as a PDF (866.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERRY M. N. THE EFFECTS OF ADENINE NUCLEOTIDES ON PYRUVATE METABOLISM IN RAT LIVER. Biochem J. 1965 Jun;95:587–596. doi: 10.1042/bj0950587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohn Z. A., Parks E. The regulation of pinocytosis in mouse macrophages. II. Factors inducing vesicle formation. J Exp Med. 1967 Feb 1;125(2):213–232. doi: 10.1084/jem.125.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohn Z. A. The regulation of pinocytosis in mouse macrophages. I. Metabolic requirements as defined by the use of inhibitors. J Exp Med. 1966 Oct 1;124(4):557–571. doi: 10.1084/jem.124.4.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARARY I., SLATER E. C. STUDIES IN VITRO ON SINGLE BEATING HEART CELLS. 8. THE EFFECT OF OLIGOMYCIN, DINITROPHENOL, AND OUABAIN ON THE BEATING RATE. Biochim Biophys Acta. 1965 May 18;99:227–233. doi: 10.1016/s0926-6593(65)80119-9. [DOI] [PubMed] [Google Scholar]
- WU R., RACKER E. Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells. J Biol Chem. 1959 May;234(5):1029–1035. [PubMed] [Google Scholar]
- ZIMMERMAN A. M., LANDAU J. V., MARSLAND D. The effects of adenosine triphosphate and dinitro-o-cresol upon the form and movement of Amoeba proteus; a pressure-temperature study. Exp Cell Res. 1958 Dec;15(3):484–495. doi: 10.1016/0014-4827(58)90096-x. [DOI] [PubMed] [Google Scholar]