Abstract
Sheep antisera specific for the three major immunoglobulin groups of the rabbit, i.e. IgG (γ-chain), IgA (α-chain), and IgM (µ-chain), are each able to induce blast transformation of the peripheral lymphocytes of the rabbit when added to in vitro cultures. The per cent of lymphocytes transformed with each antiserum indicate that one-fourth of the peripheral lymphocytes carry or have the capacity to synthesize molecules of all three of the major immunoglobulin groups, and that the remaining three-fourths carry or have the capacity to synthesize only two (IgG and IgM). The data do not permit direct conclusions concerning the ability of a single cell to produce molecules belonging to more than one immunoglobulin group at the same time, or the ability of a given cell to make a transition from the synthesis of molecules of one immunoglobulin group to those of another group.
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- ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. IV. THE DETAILED METHODS FOR MEASURING ANTIBODY SYNTHESIS BY INDIVIDUAL CELLS, THE KINETICS OF ANTIBODY FORMATION BY RABBITS AND THE PROPERTIES OF CELL SUSPENSIONS. J Immunol. 1964 Mar;92:372–390. [PubMed] [Google Scholar]
- CURTAIN C. C., BAUMGARTEN A. IMMUNOCYTOCHEMICAL LOCALIZATION OF A 19S GAMMA-1 MACROGLOBULIN COLD HAEMAGGLUTININ. Aust J Exp Biol Med Sci. 1965 Apr;43:157–162. doi: 10.1038/icb.1965.12. [DOI] [PubMed] [Google Scholar]
- Cebra J. J., Colberg J. E., Dray S. Rabbit lymphoid cells differentiated with respect to alpha-, gamma-, and mu- heavy polypeptide chains and to allotypic markers Aa1 and Aa2. J Exp Med. 1966 Mar 1;123(3):547–558. doi: 10.1084/jem.123.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hiramoto R. N., Hamlin M. Detection of two antibodies in single plasma cells by the paired fluorescence technique. J Immunol. 1965 Aug;95(2):214–224. [PubMed] [Google Scholar]
- MULLER-EBERHARD H. J. A new supporting medium for preparative electrophoresis. Scand J Clin Lab Invest. 1960;12:33–37. [PubMed] [Google Scholar]
- NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERNIS B., CHIAPPINO G. IDENTIFICATION IN HUMAN LYMPHOID TISSUES OF CELLS THAT PRODUCE GROUP 1 OR GROUP 2 GAMMA-GLOBULINS. Immunology. 1964 Sep;7:500–506. [PMC free article] [PubMed] [Google Scholar]
- Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SOLOMON A., FAHEY J. L., MALMGREN R. A. Immunohistologic localization of gamma-1-macroglobulins, beta-2A-myeloma proteins, 6.6 S gamma-myeloma proteins and Bence Jones proteins. Blood. 1963 Apr;21:403–423. [PubMed] [Google Scholar]
- Sell S., Gell P. G. Studies on rabbit lymphocytes in vitro. IV. Blast transformation of the lymphocytes from newborn rabbits induced by antiallotype serum to a paternal IgG allotype not present in the serum of the lymphocyte donors. J Exp Med. 1965 Nov 1;122(5):923–928. doi: 10.1084/jem.122.5.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sell S., Rowe D. S., Gell P. G. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum. J Exp Med. 1965 Oct 1;122(4):823–839. doi: 10.1084/jem.122.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sell S. Studies on rabbit lymphocytes in vitro. V. The induction of blast transformation with sheep antisera to rabbit IgG subunits. J Exp Med. 1967 Feb 1;125(2):289–301. doi: 10.1084/jem.125.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Furth R., Schuit H. R., Hijmans W. The formation of immunoglobulins by human tissues in vitro. IV. Circulating lymphocytes in normal and pathological conditions. Immunology. 1966 Jul;11(1):29–40. [PMC free article] [PubMed] [Google Scholar]