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. 1967 Aug 1;126(2):223–234. doi: 10.1084/jem.126.2.223

REACTIVATION IN VITRO OF IMMUNOCOMPETENCE IN IRRADIATED MOUSE SPLEEN

Amiela Globerson 1, Robert Auerbach 1
PMCID: PMC2138317  PMID: 19867297

Abstract

The restoration of immunological competence to irradiated adult mouse spleen was studied using an in vitro assay system. Competence of the spleen to evoke a graft versus host reaction in vitro was lost upon sublethal irradiation (550 R). Regeneration of immune competence occurred when such spleens were grown for 2–3 days in the presence of thymus tissue, but not when grown in the presence of a variety of other tissues. This thymic activity was demonstrated to occur across an intervening Millipore filter barrier. When lethal doses of irradiation were used, reactivation of immune competence did not occur unless both thymus and bone marrow were present. In this experimental situation the bone marrow appeared to supply the immunocompetent cells.

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Selected References

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  1. AUERBACH R. Experimental analysis of the origin of cell types in the development of the mouse thymus. Dev Biol. 1961 Jun;3:336–354. doi: 10.1016/0012-1606(61)90051-3. [DOI] [PubMed] [Google Scholar]
  2. Auerbach R., Globerson A. In vitro induction of the graft-versus-host reaction. Exp Cell Res. 1966 Apr;42(1):31–41. doi: 10.1016/0014-4827(66)90316-8. [DOI] [PubMed] [Google Scholar]
  3. DESOMER P., DENYS P., Jr, LEYTEN R. ACTIVITY OF A NONCELLULAR CALF THYMUS EXTRACT IN NORMAL AND THYMECTOMIZED MICE. Life Sci. 1963 Nov;11:810–819. doi: 10.1016/0024-3205(63)90091-2. [DOI] [PubMed] [Google Scholar]
  4. FELDMAN M., GLOBERSON A. THE ROLE OF THE THYMUS IN RESTORING IMMUNOLOGICAL REACTIVITY AND LYMPHOID CELL DIFFERENTIATION IN X-IRRADIATED ADULT MICE. Ann N Y Acad Sci. 1964 Nov 30;120:182–190. doi: 10.1111/j.1749-6632.1964.tb34716.x. [DOI] [PubMed] [Google Scholar]
  5. GLOBERSON A., FELDMAN M. ROLE OF THE THYMUS IN RESTORATION OF IMMUNE REACTIVITY AND LYMPHOID REGENERATION IN IRRADIATED MICE. Transplantation. 1964 Mar;2:212–227. doi: 10.1097/00007890-196403000-00006. [DOI] [PubMed] [Google Scholar]
  6. GLOBERSON A., FIORE-DONATI L., FELDMAN M. On the role of the thymus in recovery of immunological reactivity following x-irradiation. Exp Cell Res. 1962 Nov;28:455–457. doi: 10.1016/0014-4827(62)90305-1. [DOI] [PubMed] [Google Scholar]
  7. GREGOIRE C., DUCHATEAU G. A study on lympho-epithelial symbiosis in thymus; reactions on the lymphatic tissue to extracts and to implants of epithelial components of thymus. Arch Biol (Liege) 1956;67(2):269–296. [PubMed] [Google Scholar]
  8. GROBSTEIN C. Some transmission characteristics of the tubule-inducing influence on mouse metanephrogenic mesenchyme. Exp Cell Res. 1957 Dec;13(3):575–587. doi: 10.1016/0014-4827(57)90087-3. [DOI] [PubMed] [Google Scholar]
  9. GROBSTEIN C. Trans-filter induction of tubules in mouse metanephrogenic mesenchyme. Exp Cell Res. 1956 Apr;10(2):424–440. doi: 10.1016/0014-4827(56)90016-7. [DOI] [PubMed] [Google Scholar]
  10. Globerson A., Auerbach R. Primary antibody response in organ cultures. J Exp Med. 1966 Nov 1;124(5):1001–1016. doi: 10.1084/jem.124.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Globerson A., Auerbach R. Primary immune reactions in organ cultures. Science. 1965 Aug 27;149(3687):991–993. doi: 10.1126/science.149.3687.991. [DOI] [PubMed] [Google Scholar]
  12. Globerson A. In vitro studies on radiation lymphoid recovery of mouse spleen. J Exp Med. 1966 Jan 1;123(1):25–32. doi: 10.1084/jem.123.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldstein A. L., Slater F. D., White A. Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (thymosin). Proc Natl Acad Sci U S A. 1966 Sep;56(3):1010–1017. doi: 10.1073/pnas.56.3.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HARRIS J. E., FORD C. E., BARNES D. W., EVANS E. P. EVIDENCE FROM PARABIOSIS FOR AN AFFERENT STREAM OF CELLS. Nature. 1964 Feb 29;201:886–887. doi: 10.1038/201886a0. [DOI] [PubMed] [Google Scholar]
  15. Hand T., Caster P., Luckey T. D. Isolation of a thymus hormone, LSH. Biochem Biophys Res Commun. 1967 Jan 10;26(1):18–23. doi: 10.1016/0006-291x(67)90245-8. [DOI] [PubMed] [Google Scholar]
  16. LEVEY R. H., TRAININ N., LAW L. W. EVIDENCE FOR FUNCTION OF THYMIC TISSUE IN DIFFUSION CHAMBERS IMPLANTED IN NEONATALLY THYMECTOMIZED MICE. PRELIMINARY REPORT. J Natl Cancer Inst. 1963 Aug;31:199–217. [PubMed] [Google Scholar]
  17. LEVEY R. H., TRAININ N., LAW L. W. EVIDENCE FOR FUNCTION OF THYMIC TISSUE IN DIFFUSION CHAMBERS IMPLANTED IN NEONATALLY THYMECTOMIZED MICE. PRELIMINARY REPORT. J Natl Cancer Inst. 1963 Aug;31:199–217. [PubMed] [Google Scholar]
  18. Law L. W. Studies of thymic function with emphasis on the role of the thymus in oncogenesis. Cancer Res. 1966 Apr;26(4):551–574. [PubMed] [Google Scholar]
  19. MILLER J. F., LEUCHARS E., CROSS A. M., DUKOR P. IMMUNOLOGIC ROLE OF THE THYMUS IN RADIATION CHIMERAS. Ann N Y Acad Sci. 1964 Nov 30;120:205–217. doi: 10.1111/j.1749-6632.1964.tb34718.x. [DOI] [PubMed] [Google Scholar]
  20. Miller J. F., Osoba D., Dukor P. A humoral thymus mechanism responsible for immunologic maturation. Ann N Y Acad Sci. 1965 Jun 30;124(1):95–104. doi: 10.1111/j.1749-6632.1965.tb18945.x. [DOI] [PubMed] [Google Scholar]
  21. OSOBA D., MILLER J. F. EVIDENCE FOR A HUMORAL THYMUS FACTOR RESPONSIBLE FOR THE MATURATION OF IMMUNOLOGICAL FACULTY. Nature. 1963 Aug 17;199:653–654. doi: 10.1038/199653a0. [DOI] [PubMed] [Google Scholar]
  22. PARROTT D. M., EAST J. THE THYMUS AND IMMUNITY. THE IMMUNOLOGICAL STATUS OF THYMECTOMIZED ANIMALS. A SURVEY. Proc R Soc Med. 1964 Feb;57:147–151. doi: 10.1177/003591576405700229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SIMONSEN M. Graft versus host reactions. Their natural history, and applicability as tools of research. Prog Allergy. 1962;6:349–467. [PubMed] [Google Scholar]

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