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. 1967 Jan 1;125(1):33–44. doi: 10.1084/jem.125.1.33

GAMMA GLOBULIN AND ANTIBODY FORMATION IN VITRO

VI. EFFECT OF X-IRRADIATION ON THE SECONDARY ANTIBODY RESPONSE IN VITRO

Vera J Stecher 1, G Jeanette Thorbecke 1
PMCID: PMC2138340  PMID: 4163360

Abstract

The present studies have shown that the influence of X-irradiation on the secondary antibody response in vitro is remarkably similar to its effect on the primary response in vivo. When sensitized tissue was first irradiated and then reexposed to antigen, the duration of the interval between irradiation and antigen addition determined the degree of inhibition of the secondary response obtained. A delay of 12 hr resulted in stronger inhibition than a delay of 6 hr, and an interval of 24 hr before reexposure to antigen caused complete suppression of antibody production to diphtheria toxoid and almost complete suppression when sheep RBC were used as the antigen. Induction of the secondary response in rabbit lymph node tissue in vitro followed by exposure to X-irradiation, revealed that immediate exposure to irradiation after antigen produced stronger inhibition of the subsequent response than irradiation on days 2–3. Irradiation on day 6 had no detectable effect. The effectiveness of the early radiation is probably due to prevention of the proliferation of the antibody-forming cells. BUDR was found to be effective at similar time periods as X-irradiation, whereas colchicine could still stop antibody formation when added late during the secondary response in vitro. It was noted that lymph nodes from some BSA-sensitized rabbits as late as 18 months after sensitization gave a response indistinguishable from a typical secondary response, even when not reexposed to antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBROSE C. T., COONS A. H. Studies on antibody production. VIII. The inhibitory effect of chloramphenicol on the synthesis of antibody in tissue culture. J Exp Med. 1963 Jun 1;117:1075–1088. doi: 10.1084/jem.117.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berenbaum M. C. Radiosensitivity of immunologically activated cells. Nature. 1966 Mar 26;209(5030):1313–1315. doi: 10.1038/2091313a0. [DOI] [PubMed] [Google Scholar]
  3. CROSLAND-TAYLOR P. J. The effect of x-rays on the secondary antibody response. Br J Exp Pathol. 1955 Oct;36(5):530–533. [PMC free article] [PubMed] [Google Scholar]
  4. DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
  5. DIXON F. J., McCONAHEY P. J. Enhancement of antibody formation by whole body x-radiation. J Exp Med. 1963 May 1;117:833–848. doi: 10.1084/jem.117.5.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DIXON F. J., TALMAGE D. W., MAURER P. H. Radiosensitive and radioresistant phases in the antibody response. J Immunol. 1952 Jun;68(6):693–700. [PubMed] [Google Scholar]
  7. DRAPER L. R., SUSSDORF D. H. The primary hemolysin response in rabbits following shielding from x rays of x irradiation of the spleen, appendix, liver or hind legs. J Infect Dis. 1956 Sep-Oct;99(2):129–142. doi: 10.1093/infdis/99.2.129. [DOI] [PubMed] [Google Scholar]
  8. DUTTON R. W., PEARCE J. D. A survey of the effect of metabolic antagonists on the synthesis of antibody in an in vitro system. Immunology. 1962 May;5:414–423. [PMC free article] [PubMed] [Google Scholar]
  9. FISHMAN M. Antibody formation in vitro. J Exp Med. 1961 Dec 1;114:837–856. doi: 10.1084/jem.114.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GOWANS J. L. The fate of parental strain small lymphocytes in F1 hybrid rats. Ann N Y Acad Sci. 1962 Oct 24;99:432–455. doi: 10.1111/j.1749-6632.1962.tb45326.x. [DOI] [PubMed] [Google Scholar]
  11. Garvey J. S., Campbell D. H. Autoradiographic investigation of tissues after primary and multiple antigenic stimulation of rabbits. Nature. 1966 Mar 19;209(5029):1201–1202. doi: 10.1038/2091201a0. [DOI] [PubMed] [Google Scholar]
  12. Hochwald G. M., Thorbecke G. J., Asofsky R. SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'(3): I. A NEW TECHNIQUE FOR THE DEMONSTRATION OF THE SYNTHESIS OF INDIVIDUAL SERUM PROTEINS BY TISSUES IN VITRO. J Exp Med. 1961 Sep 30;114(4):459–470. doi: 10.1084/jem.114.4.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KEUNING F. J., van der MEER, NIEUWENHUIS P., OUDENDIJK P. The histophysiology of the antibody response. II. Antibody responses and splenic plasma cell reaction in sublethally x-irradiated rabbits. Lab Invest. 1963 Feb;12:156–170. [PubMed] [Google Scholar]
  14. MAKINODAN T., KASTENBAUM M. A., PETERSON W. J. Radiosensitivity of spleen cells from normal and preimmunized mice and its significance to intact animals. J Immunol. 1962 Jan;88:31–37. [PubMed] [Google Scholar]
  15. MICHAELIDES M. C., COONS A. H. Studies on antibody production. V. The secondary response in vitro. J Exp Med. 1963 Jun 1;117:1035–1051. doi: 10.1084/jem.117.6.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MOLLER G., WIGZELL H. ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL. ANTIBODY-INDUCED SUPPRESSION OF 19S AND 7S ANTIBODY RESPONSE. J Exp Med. 1965 Jun 1;121:969–989. doi: 10.1084/jem.121.6.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Brien T. F., Coons A. H. STUDIES ON ANTIBODY PRODUCTION : VII. THE EFFECT OF 5-BROMODEOXYURIDINE ON THE IN VITRO ANAMNESTIC ANTIBODY RESPONSE. J Exp Med. 1963 Jun 1;117(6):1063–1074. doi: 10.1084/jem.117.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PORTER R. J. Prolonged suppression by x-ray of adaptation for the secondary antibody response. Proc Soc Exp Biol Med. 1962 Dec;111:583–584. doi: 10.3181/00379727-111-27861. [DOI] [PubMed] [Google Scholar]
  19. PORTER R. J. Studies on antibody formation. Effects of x-irradiation on adaptation for the secondary response of rabbits to bovine gamma-globulin. J Immunol. 1960 May;84:485–490. [PubMed] [Google Scholar]
  20. SCHREK R., STEFANI S. RESISTANCE OF PHYTOHAEMAGGLUTININ-TREATED LYMPHOCYTES TO X-RAYS AND NITROGEN MUSTARD. Nature. 1963 Nov 2;200:482–483. doi: 10.1038/200482a0. [DOI] [PubMed] [Google Scholar]
  21. SILVERMAN M. S., CHIN P. H. Quantitative serological determination of antibody formation in x-irradiated rabbits. J Immunol. 1954 Aug;73(2):120–124. [PubMed] [Google Scholar]
  22. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  23. STRAUCH D., STENDER H. S., WINTER H. Effects of a nonspecific stimulation on tissue radiosensitivity during the course of antibody production. J Immunol. 1959 Apr;82(4):298–303. [PubMed] [Google Scholar]
  24. TALIAFERRO W. H., TALIAFERRO L. O. Further studies on the radiosensitive stages in hemolysin formation. J Infect Dis. 1954 Sep-Oct;95(2):134–141. doi: 10.1093/infdis/95.2.134. [DOI] [PubMed] [Google Scholar]
  25. THORBECKE G. J., JACOBSON E. B., ASOFSKY R. GAMMA. J Immunol. 1964 May;92:734–746. [PubMed] [Google Scholar]
  26. TROWELL O. A. The sensitivity of lymphocytes to ionising radiation. J Pathol Bacteriol. 1952 Oct;64(4):687–704. doi: 10.1002/path.1700640403. [DOI] [PubMed] [Google Scholar]

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