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. 1967 Jan 1;125(1):149–162. doi: 10.1084/jem.125.1.149

EXPERIMENTAL ALLERGIC GLOMERULONEPHRITIS INDUCED IN THE RABBIT WITH HETEROLOGOUS RENAL ANTIGENS

Emil R Unanue 1, Frank J Dixon 1
PMCID: PMC2138345  PMID: 5334542

Abstract

Rabbits immunized to different heterologous renal antigens developed antibodies some of which are fixed to their own glomeruli (autoantibodies). These autoantibodies, reacting with the host's kidneys, were directed to those antigenic determinants which were present in identical or cross-reactive form in both the immunizing antigen and the kidneys of the host. Glomerulonephritis developed in some of the rabbits immunized to heterologous mammalian kidneys, but in none of those immunized to nonmammalian kidneys. The development of glomerulonephritis in the immunized rabbits appears to depend upon two factors: (a) the amount of autoantikidney antibody made to the common renal antigens; and (b) the quantity of the common antigens available in the host's kidneys. An amount of common antigen sufficient to fix a nephritogenic amount of antibody is essential for the development of disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHERSON G. L., DUMONDE D. C. Autoantibody production in rabbits. II. Organ-specific autontibody in rabbits injected with rat tissues. Immunology. 1963 Jan;6:19–29. [PMC free article] [PubMed] [Google Scholar]
  2. BLOZIS G. G., SPARGO B., ROWLEY D. A. Glomerular basement membrane changes with the nephrotic syndrome produced in the rat by homologous kidney and hemophilus pertusis vaccine. Am J Pathol. 1962 Feb;40:153–165. [PMC free article] [PubMed] [Google Scholar]
  3. Furth J., Kabat E. A., Assistance of Jean Brundage and Mary C. Boon IMMUNOLOGICAL SPECIFICITY OF MATERIAL SEDIMENTABLE AT HIGH SPEED PRESENT IN NORMAL AND TUMOR TISSUES. J Exp Med. 1941 Aug 31;74(3):247–256. doi: 10.1084/jem.74.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GERY I., YAGI Y., PRESSMAN D. REACTIONS OF LOCALIZING ANTI-KIDNEY ANTIBODIES WITH TISSUES OF VARIOUS SPECIES. J Immunol. 1965 Jun;94:950–958. [PubMed] [Google Scholar]
  5. HEYMANN W., HACKEL D. B., HARWOOD S., WILSON S. G., HUNTER J. L. Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med. 1959 Apr;100(4):660–664. doi: 10.3181/00379727-100-24736. [DOI] [PubMed] [Google Scholar]
  6. HUMPHREY J. H. The pathogenesis of glomerulonephritis; a reinvestigation of the auto-immunization hypothesis. J Pathol Bacteriol. 1948 Apr;60(2):211–218. doi: 10.1002/path.1700600208. [DOI] [PubMed] [Google Scholar]
  7. Hess E. V., Ashworth C. T., Ziff M. Nephrosis in the rat induced by rat kidney extracts. Ann N Y Acad Sci. 1965 Jun 30;124(1):323–328. doi: 10.1111/j.1749-6632.1965.tb18967.x. [DOI] [PubMed] [Google Scholar]
  8. KEKWICK R. A., MACKAY M. E., NANCE M. H., RECORD B. R. The purification of human fibrinogen. Biochem J. 1955 Aug;60(4):671–683. doi: 10.1042/bj0600671b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KLEIN P., BURKHOLDER P. [A method for fluorescence-optic demonstration of complement fixation and its use in the histo-immunological demonstration of experimental kidney anaphylaxis]. Dtsch Med Wochenschr. 1959 Nov 6;84:2001–2004. doi: 10.1055/s-0028-1114563. [DOI] [PubMed] [Google Scholar]
  10. KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
  11. Lachmann P. J., Liske R. The preparation and properties of alexinated intermediates that react with conglutinin. II. Equine, rabbit and human complement. Immunology. 1966 Sep;11(3):255–262. [PMC free article] [PubMed] [Google Scholar]
  12. Lerner R. A., Dixon F. J. Transfer of ovine experimental allergic glomerulonephritis (EAG) with serum. J Exp Med. 1966 Sep 1;124(3):431–442. doi: 10.1084/jem.124.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MARDINEY M. R., Jr, MUELLER-EBERHARD H. J. MOUSE BETA-1C-GLOBULIN: PRODUCTION OF ANTISERUM AND CHARACTERIZATION IN THE COMPLEMENT REACTION. J Immunol. 1965 Jun;94:877–882. [PubMed] [Google Scholar]
  14. MARKOWITZ A. S. Interactions of anti-glomerular basement membrane antisera. Immunology. 1960 Apr;3:117–125. [PMC free article] [PubMed] [Google Scholar]
  15. MIDDLETON E., Jr, MIDDLETON E. B., SEEGAL B. C. Effect of injecting rats with homologous renal tissue mixed with adjuvants or streptococci. AMA Arch Pathol. 1953 Aug;56(2):125–134. [PubMed] [Google Scholar]
  16. MILGROM F., CENTENO E., SHULMAN S., WITEBSKY E. AUTOANTIBODIES RESULTING FROM IMMUNIZATION WITH KIDNEY. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1009–1013. doi: 10.3181/00379727-116-29434. [DOI] [PubMed] [Google Scholar]
  17. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  18. SIMONSEN M. Studies on the pathogenesis of experimental glomerulonephritis. Acta Pathol Microbiol Scand. 1953;32(1):85–108. doi: 10.1111/j.1699-0463.1953.tb00231.x. [DOI] [PubMed] [Google Scholar]
  19. STEBLAY R. W. Glomerulonephritis induced in monkeys by injections of heterologous glomerular basement membrane and Freund's adjuvant. Nature. 1963 Mar 23;197:1173–1176. doi: 10.1038/1971173a0. [DOI] [PubMed] [Google Scholar]
  20. STEBLAY R. W. Glomerulonephritis induced in sheep by injections of heterologous glomerular basement membrane and Freund's complete adjuvant. J Exp Med. 1962 Aug 1;116:253–272. doi: 10.1084/jem.116.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schwentker F. F., Comploier F. C. THE PRODUCTION OF KIDNEY ANTIBODIES BY INJECTION OF HOMOLOGOUS KIDNEY PLUS BACTERIAL TOXINS. J Exp Med. 1939 Aug 31;70(3):223–230. doi: 10.1084/jem.70.3.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. V. STUDIES ON THE INTERACTION OF NEPHROTOXIC ANTIBODIES WITH TISSUE OF THE RAT. J Exp Med. 1965 May 1;121:697–714. doi: 10.1084/jem.121.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. VI. THE AUTOLOGOUS PHASE OF NEPHROTOXIC SERUM NEPHRITIS. J Exp Med. 1965 May 1;121:715–725. doi: 10.1084/jem.121.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Unanue E. R., Dixon F. J., Feldman J. D. Experimental allergic glomerulonephritis induced in the rabbit with homologous renal antigens. J Exp Med. 1967 Jan 1;125(1):163–176. doi: 10.1084/jem.125.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]

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