Abstract
Many sera from normal individuals as well as patients with various disease states contain agglutinating antibodies which show specificity for antigenic determinants of γ-globulin revealed by pepsin digestion at pH 4.1. Sera containing such agglutinating activity as well as sera negative for these agglutinators contain low molecular weight (3S–5S) components of slow γ-mobility which inhibit these agglutination reactions. Low molecular weight inhibitors show both auto- and isospecificity, and are antigenically related to the 5S pepsin fragment of γ-globulin. A common situation is thereby revealed in which human anti-γ-globulin antibodies showing specificity for pepsin-digested γ-globulins are present in serum along with low molecular weight γ-globulin components capable of inhibition. Autoreactivity or autospecificity of such anti-γ-globulin factors is a phenomenon shared by both normal human sera and sera from patients with various disease states.
Full Text
The Full Text of this article is available as a PDF (817.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FIREMAN P., HERSHGOLD E., CORDOBA F., SCHMID K., GITLIN D. LOW MOLECULAR WEIGHT GAMMA-GLOBULINS OF URINE AND PLASMA AND THEIR RELATION TO 7S GAMMA-2-GLOBULIN. Nature. 1964 Jul 4;203:78–80. doi: 10.1038/203078a0. [DOI] [PubMed] [Google Scholar]
- HARBOE M., RAU B., AHO K. PROPERTIES OF VARIOUS ANTI-GAMMA-GLOBULIN FACTORS IN HUMAN SERA. J Exp Med. 1965 Apr 1;121:503–519. doi: 10.1084/jem.121.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELMKAMP R. W., GOODLAND R. L., BALE W. F., SPAR I. L., MUTSCHLER L. E. High specific activity iodination of gamma-globulin with iodine-131 monochloride. Cancer Res. 1960 Nov;20:1495–1500. [PubMed] [Google Scholar]
- IKENAKA T., GITLIN D., SCHMID K. PREPARATION AND CHARACTERIZATION OF THE LOW MOLECULAR WEIGHT HUMAN PLASMA 3S GAMMA-1-GLOBULINS. J Biol Chem. 1965 Jul;240:2868–2876. [PubMed] [Google Scholar]
- KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
- MOORE B. P., LININS I., McINTYRE J. A serum with an albumin-active, auto-agglutinating property. Blood. 1959 Apr;14(4):364–368. [PubMed] [Google Scholar]
- Natvig J. B. Heterogeneity of anti-antibodies in individual human sera. Acta Pathol Microbiol Scand. 1966;66(2):188–196. doi: 10.1111/apm.1966.66.2.188. [DOI] [PubMed] [Google Scholar]
- Natvig J. B. Heterogeneity of anti-gamma-globulin factors detected by pepsin-digested human gamma G-globulin. Acta Pathol Microbiol Scand. 1966;66(3):369–382. doi: 10.1111/apm.1966.66.3.369. [DOI] [PubMed] [Google Scholar]
- Natvig J. B. Reaction of human anti-antibodies with incomplete anti-D and its sub-units. Acta Pathol Microbiol Scand. 1966;66(3):383–395. doi: 10.1111/apm.1966.66.3.383. [DOI] [PubMed] [Google Scholar]
- OSTERLAND C. K., HARBOE M., KUNKEL H. G. Anti-gamma-globulin factors in human sera revealed by enzymatic splitting of anti-Rh antibodies. Vox Sang. 1963 Mar-Apr;8:133–152. doi: 10.1111/j.1423-0410.1963.tb03290.x. [DOI] [PubMed] [Google Scholar]
- PORATH J., UI N. CHEMICAL STUDIES ON IMMUNOGLOBULINS. I. A NEW PREPARATIVE PROCEDURE FOR GAMMA-GLOBULINS EMPLOYING GLYCINE-RICH SOLVENT SYSTEMS. Biochim Biophys Acta. 1964 Aug 19;90:324–333. [PubMed] [Google Scholar]
- ROBERT B., DENES Y., CREPIN Y. MISE EN 'EVIDENCE DE PEPTIDES DIALYSABLES ACCOMPAGNANT LES GAMMA-GLOBULINES DANS LEUR PR'EPARATION. C R Hebd Seances Acad Sci. 1965 Jan 11;260:734–737. [PubMed] [Google Scholar]
- SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spiegelberg H. L., Weigle W. O. The in vivo formation and fate of antigen-antibody complexes formed by fragments and polypeptide chains of rabbit gamma-G-antibodies. J Exp Med. 1966 Jun 1;123(6):999–1012. doi: 10.1084/jem.123.6.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams R. C., Jr, Brunning R. D., Wollheim F. A. Light-chain disease. An abortive variant of multiple myeloma. Ann Intern Med. 1966 Sep;65(3):471–486. doi: 10.7326/0003-4819-65-3-471. [DOI] [PubMed] [Google Scholar]
- Williams R. C., Jr, Lawrence T. G., Jr Variations among gamma-globulins at the antigenic site revealed by pepsin digestion. J Clin Invest. 1966 May;45(5):714–723. doi: 10.1172/JCI105386. [DOI] [PMC free article] [PubMed] [Google Scholar]