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. 1967 Mar 31;125(4):629–646. doi: 10.1084/jem.125.4.629

IN VITRO TRANSFORMATION BY THE ADENOVIRUS-SV40 HYBRID VIRUSES

II. CHARACTERISTICS OF THE TRANSFORMATION OF HAMSTER CELLS BY THE ADENO 2-, ADENO 3-, AND ADENO 12-SV40 VIRUSES

Paul H Black 1, Beverly J White 1
PMCID: PMC2138372  PMID: 4289741

Abstract

Primary weanling hamster kidney cultures were transformed with the adeno 2-SV40, adeno 3-SV40, and adeno 12-SV40 hybrid viruses and with adenovirus type 12. The transformed cell lines which were established were characterized with respect to morphology, virus and antigen content, and chromosome aberrations. The adeno 2 and adeno 3-SV40 hybrid transformed cells had the morphology and T antigen content characteristic of SV40 transformations; cells transformed by the former hybrid had cytogenetic changes typical of SV40-transformed cells as well. The adeno 12-SV40 transformed cells were similar morphologically to adeno 12-transformed cells, contained both the SV40 and adeno T antigens and demonstrated the karyotypic instability of SV40-transformed cells, indicating that both viral genomes are operative in these cells. Although the results indicate that the SV40 genome in hybrids derived from the moderately or nononcogenic adenoviruses supplies the determinants for most of the characteristics investigated, and perhaps for oncogenesis, evidence was presented which suggests that a portion of a nononcogenic adenovirus genome may be integrated in adeno 2-SV40 transformed cells and directs the synthesis of adenovirus T antigens.

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Selected References

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  1. BLACK P. H., ROWE W. P. AN ANALYSIS OF SV40-INDUCED TRANSFORMATION OF HAMSTER KIDNEY TISSUE IN VITRO. I. GENERAL CHARACTERISTICS. Proc Natl Acad Sci U S A. 1963 Oct;50:606–613. doi: 10.1073/pnas.50.4.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLACK P. H., ROWE W. P., TURNER H. C., HUEBNER R. J. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Dec;50:1148–1156. doi: 10.1073/pnas.50.6.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLACK P. H., ROWE W. P. VIRAL STUDIES OF SV40 TUMORIGENESIS IN HAMSTERS. J Natl Cancer Inst. 1964 Jan;32:253–265. [PubMed] [Google Scholar]
  4. Baum S. G., Reich P. R., Hybner C. J., Rowe W. P., Weissman S. M. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509–1515. doi: 10.1073/pnas.56.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Black P. H. An analysis of SV40-induced transformation of hamster kidney tissue in vitro. 3. Persistence of SV40 viral genome in clones of transformed hamster cells. J Natl Cancer Inst. 1966 Oct;37(4):487–493. [PubMed] [Google Scholar]
  6. Black P. H., Berman L. D., Maloof R. An analysis of SV40-induced transformation of hamster kidney tissue in vitro. IV. Studies of the pathology of hamster tumors induced with SV40-transformed hamster cell clones. J Natl Cancer Inst. 1966 Oct;37(4):495–504. [PubMed] [Google Scholar]
  7. Black P. H., Todaro G. J. In vitro transformation of hamster and human cells with the adeno 7-SV 40 hybrid virus. Proc Natl Acad Sci U S A. 1965 Aug;54(2):374–381. doi: 10.1073/pnas.54.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. COOPER H. L., BLACK P. H. CYTOGENETIC STUDIES OF THREE CLONES DERIVED FROM A PERMANENT LINE OF HAMSTER CELLS TRANSFORMED BY SV40. J Cell Physiol. 1964 Oct;64:201–219. doi: 10.1002/jcp.1030640206. [DOI] [PubMed] [Google Scholar]
  9. COOPER H. L., BLACK P. H. Cytogenetic studies of hamster kidney cell cultures transformed by the simian vacuolating virus (SV40). J Natl Cancer Inst. 1963 May;30:1015–1043. [PubMed] [Google Scholar]
  10. FJELDE A., TRENTIN J. J., BRYAN E. THE CHROMOSOMES OF TUMORS INDUCED IN MICE BY HUMAN ADENOVIRUS, TYPE 12. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1102–1105. doi: 10.3181/00379727-116-29464. [DOI] [PubMed] [Google Scholar]
  11. HOGGAN M. D., ROWE W. P., BLACK P. H., HUEBNER R. J. PRODUCTION OF "TUMOR-SPECIFIC" ANTIGENS BY ONCOGENIC VIRUSES DURING ACUTE CYTOLYTIC INFECTIONS. Proc Natl Acad Sci U S A. 1965 Jan;53:12–19. doi: 10.1073/pnas.53.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  13. HUEBNER R. J., CHANOCK R. M., RUBIN B. A., CASEY M. J. INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS. Proc Natl Acad Sci U S A. 1964 Dec;52:1333–1340. doi: 10.1073/pnas.52.6.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HUEBNER R. J., ROWE W. P., TURNER H. C., LANE W. T. SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS. Proc Natl Acad Sci U S A. 1963 Aug;50:379–389. doi: 10.1073/pnas.50.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Igel H. J., Black P. H. In vitro transformation by the adenovirus-SV40 hybrid viruses. 3. Morphology of tumors induced with transformed cells. J Exp Med. 1967 Apr 1;125(4):647–656. doi: 10.1084/jem.125.4.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lewis A. M., Jr, Baum S. G., Prigge K. O., Rowe W. P. Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc Soc Exp Biol Med. 1966 May;122(1):214–218. doi: 10.3181/00379727-122-31093. [DOI] [PubMed] [Google Scholar]
  17. Lewis A. M., Jr, Prigge K. O., Rowe W. P. Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome. Proc Natl Acad Sci U S A. 1966 Mar;55(3):526–531. doi: 10.1073/pnas.55.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MOORHEAD P. S., SAKSELA E. NON-RANDOM CHROMOSOMAL ABERRATIONS IN SV 40-TRANSFORMED HUMAN CELLS. J Cell Physiol. 1963 Aug;62:57–83. doi: 10.1002/jcp.1030620109. [DOI] [PubMed] [Google Scholar]
  19. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. RABSON A. S., O'CONOR G. T., BEREZESKY I. K., PAUL F. J. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40. Proc Soc Exp Biol Med. 1964 May;116:187–190. doi: 10.3181/00379727-116-29197. [DOI] [PubMed] [Google Scholar]
  22. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rapp F., Butel J. S., Melnick J. L. SV40-adenovirus "hybrid" populations: transfer of SV40 determinants from one type of adenovirus to another. Proc Natl Acad Sci U S A. 1965 Sep;54(3):717–724. doi: 10.1073/pnas.54.3.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proc Natl Acad Sci U S A. 1966 Feb;55(2):336–341. doi: 10.1073/pnas.55.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rowe W. P., Baum S. G., Pugh E., Hoggan M. D. Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J Exp Med. 1965 Nov 1;122(5):943–954. doi: 10.1084/jem.122.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rowe W. P., Pugh W. E. Studies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials. Proc Natl Acad Sci U S A. 1966 May;55(5):1126–1132. doi: 10.1073/pnas.55.5.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rowe W. P. Studies of adenovirus-SV40 hybrid viruses. 3. Transfer of SV40 gene between adenovirus types. Proc Natl Acad Sci U S A. 1965 Sep;54(3):711–717. doi: 10.1073/pnas.54.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stich H. F., Yohn D. S. Viruses and mammalian chromosomes. V. Chromosome aberrations in tumors of Syrian hamsters induced by adenovirus type 12. J Natl Cancer Inst. 1965 Oct;35(4):603–615. [PubMed] [Google Scholar]
  30. Takemoto K. K., Habel K. Hamster ascitic fluids containing complement-fixing antibody against virus-induced tumor antigens. Proc Soc Exp Biol Med. 1965 Oct;120(1):124–127. doi: 10.3181/00379727-120-30464. [DOI] [PubMed] [Google Scholar]
  31. Utsumi K. R., Kitamura I., Trentin J. J. Karyologic studies of normal cells and of adenovirus-type-12-induced tumor cells of the Syrian hamster. J Natl Cancer Inst. 1965 Nov;35(5):759–769. [PubMed] [Google Scholar]

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