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. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423

IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICE

Robert I Mishell 1, Richard W Dutton 1
PMCID: PMC2138377  PMID: 6034749

Abstract

A culture system for cell suspensions from mouse spleens has been described. The system provides adequate conditions for in vitro immunization on initial exposure to heterologous erythrocytes. The in vitro response closely parallels that observed in vivo with respect to size, early kinetics, antigen dose, and the inhibitory effect of passive antibody. The response of cultured cells differs in two respects from that seen in vivo. There is an increase in the ability to discriminate between different varieties of homologous erythrocytes and the in vitro response does not appear to be limited by whatever mechanisms regulate the in vivo response.

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Selected References

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  1. ASKONAS B. A., RHODES J. M. IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES. Nature. 1965 Jan 30;205:470–474. doi: 10.1038/205470a0. [DOI] [PubMed] [Google Scholar]
  2. Adler F. L., Fishman M., Dray S. Antibody formation initiated in vitro. 3. Antibody formation and allotypic specificity directed by ribonucleic acid from peritoneal exudate cells. J Immunol. 1966 Oct;97(4):554–558. [PubMed] [Google Scholar]
  3. BROSEMER R. W., RUTTER W. J. The effect of oxygen tension on the growth and metabolism of a mammalian cell. Exp Cell Res. 1961 Oct;25:101–113. doi: 10.1016/0014-4827(61)90311-1. [DOI] [PubMed] [Google Scholar]
  4. Bussard A. E. Antibody formation in nonimmune mouse peritoneal cells after incubation in gum containing antigen. Science. 1966 Aug 19;153(3738):887–888. doi: 10.1126/science.153.3738.887. [DOI] [PubMed] [Google Scholar]
  5. Cahn R. D. Detergents in membrane filters. Science. 1967 Jan 13;155(3759):195–196. doi: 10.1126/science.155.3759.195. [DOI] [PubMed] [Google Scholar]
  6. Dixon F. J., Jacot-Guillarmod H., McConahey P. J. The effect of passively administered antibody on antibody synthesis. J Exp Med. 1967 Jun 1;125(6):1119–1135. doi: 10.1084/jem.125.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dutton R. W., Mishell R. I. Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique. J Exp Med. 1967 Sep 1;126(3):443–454. doi: 10.1084/jem.126.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FISHMAN M., ADLER F. L. Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen. J Exp Med. 1963 Apr 1;117:595–602. doi: 10.1084/jem.117.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FISHMAN M. Antibody formation in tissue culture. Nature. 1959 Apr 25;183(4669):1200–1201. doi: 10.1038/1831200a0. [DOI] [PubMed] [Google Scholar]
  10. Friedman H. P., Stavitsky A. B., Solomon J. M. Induction in vitro of antibodies to phage T2: antigens in the RNA extract employed. Science. 1965 Sep 3;149(3688):1106–1107. doi: 10.1126/science.149.3688.1106. [DOI] [PubMed] [Google Scholar]
  11. Globerson A., Auerbach R. Primary antibody response in organ cultures. J Exp Med. 1966 Nov 1;124(5):1001–1016. doi: 10.1084/jem.124.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MAKINODAN T., KASTENBAUM M. A., PETERSON W. J. Radiosensitivity of spleen cells from normal and preimmunized mice and its significance to intact animals. J Immunol. 1962 Jan;88:31–37. [PubMed] [Google Scholar]
  13. Mishell R. I., Dutton R. W. Immunization of normal mouse spleen cell suspensions in vitro. Science. 1966 Aug 26;153(3739):1004–1006. doi: 10.1126/science.153.3739.1004. [DOI] [PubMed] [Google Scholar]
  14. RASMUSEN B. A. Blood groups in sheep. Ann N Y Acad Sci. 1962 May 3;97:306–319. doi: 10.1111/j.1749-6632.1962.tb34645.x. [DOI] [PubMed] [Google Scholar]
  15. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SAHIAR K., SCHWARTZ R. S. INHIBITION OF 19S ANTIBODY SYNTHESIS BY 7S ANTIBODY. Science. 1964 Jul 24;145(3630):395–397. doi: 10.1126/science.145.3630.395. [DOI] [PubMed] [Google Scholar]
  17. SCHMID D. O., BUSCHMANN H. The detection of blood group factors and serum transferrins in fetal blood of cattle. Ann N Y Acad Sci. 1962 May 3;97:281–284. doi: 10.1111/j.1749-6632.1962.tb34642.x. [DOI] [PubMed] [Google Scholar]
  18. Saunders G. C., King D. W. Antibody synthesis initiated in vitro by paired explants of spleen and thymus. Science. 1966 Mar 18;151(3716):1390–1391. doi: 10.1126/science.151.3716.1390. [DOI] [PubMed] [Google Scholar]
  19. Tao T. W., Uhr J. W. Primary-type antibody response in vitro. Science. 1966 Mar 4;151(3714):1096–1098. doi: 10.1126/science.151.3714.1096. [DOI] [PubMed] [Google Scholar]
  20. UHR J. W., BAUMANN J. B. Antibody formation. I. The suppression of antibody formation by passively administered antibody. J Exp Med. 1961 May 1;113:935–957. doi: 10.1084/jem.113.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wigzell H. Antibody synthesis at the cellular level. Antibody-induced suppression of 7S antibody synthesis. J Exp Med. 1966 Nov 1;124(5):953–969. doi: 10.1084/jem.124.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]

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