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. 1967 Oct 31;126(5):833–849. doi: 10.1084/jem.126.5.833

FACTORS INFLUENCING HEMATOPOIETIC SPLEEN COLONY FORMATION IN IRRADIATED MICE

I. THE NORMAL PATTERN OF ENDOGENOUS COLONY FORMATION

John C Marsh 1, Dane R Boggs 1, Carter R Bishop 1, Paul A Chervenick 1, George E Cartwright 1, Maxwell M Wintrobe 1
PMCID: PMC2138407  PMID: 6062002

Abstract

Data pertaining to the endogenous mouse spleen colony system, 10 days postirradiation, are presented. The Do for visible colonies is 78 R, while that for 6 hr iron uptake over a range of 600–800 R is 50 R. The Do for spleen weight is 196 R and that for IUdR uptake is 193 R. These measurements increase with the age of the mouse. Hypertransfusion decreases spleen iron uptake and colony number. DF-32P and sodium sulfate-35S are not useful indicators of splenic hematopoiesis in this system. Visible hematopoietic colonies in the spleen are not produced by vinblastine, nitrogen mustard, methotrexate, or cyclophosphamide.

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Selected References

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